The expression of protein kinase B in gastric cancer cell apoptosis induced 12-O-tetradecanoylphorbol-1,3-acetate

• Autorzy: Zhang B , Xia C.
• Języki publikacji: EN

Abstrakty

EN

Protein kinase B (PKB/Akt) is a serine-threonine kinase functioning downstream of phosphatidylinositol 3-kinase (PI-3 kinase) in response to mitogen or growth factor stimulation. In several cell types, it plays an important anti-apoptotic role. TPA is a potent regulator of the growth of many different cell types. Here, we detected that TPA could induce cell apoptosis in the gastric cancer cell line, BGC-823. We also found that TPA inhibited the expression of PKB/Akt in a TPA concentration- and time-dependent manner. Furthermore, TPA inhibited the phosphorylation of PKB at Ser473, but did not affect the phosphorylation of Thr308. It only attenuated the expression of PKB/Akt and the phosphorylation of Ser473 in the cell nucleus, whereas it did not change the PKB/Akt distribution in BGC-823 cells. These results suggest that PKB/Akt inhibition by TPA may be the important factor in the mechanism of effect of TPA on gastric cell lines.

Słowa kluczowe

EN

protein expression; protein kinase B; gastric cancer; cancer cell; apoptosis; 12-0-tetradecanoylphorbol-1,3-acetate; cell apoptosis

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2009
• Tom: 14
• Numer: 3
• Opis fizyczny: p.466-480,fig.,ref.

Twórcy

autor

Zhang B

• Xiamen University, Xiamen 361005, Fujian Province, P.R.China

autor

Xia C.

Bibliografia

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