Identification of Colletotrichum acutatum from yellow and Andean lupin seeds using PCR assay

• Autorzy: Kulik T. , Pszczolkowska A. , Olszewski J. , Fordonski G. , Plodzien K. , Sawicka-Sienkiewicz E.
• Języki publikacji: EN

Abstrakty

EN

PCR assays were used to identify Colletotrichum acutatum and C. gloeosporioides out of 14 fungal cultures obtained from seed samples of selected yellow and Andean lupin cultivars. The results allowed for a classification of all fungal cultures to C. acutatum. No C. gloeosporioides culture was identified. In addition, the comparison of rDNA sequence fragment from one of the cultures tested with sequences available in the NCBI/GeneBank database confirmed that the species identified was C. acutatum. The BIO-PCR technique was also used to detect potential inoculum of both species in two lupin seed samples. The results of BIO-PCR assays showed that two lupin seed samples were infected with C. acutatum while no isolate of C. gloeosporioides was detected.

Słowa kluczowe

EN

yellow lupin; lupin cultivar; agriculture; Colletotrichum gloeosporioides; Colletotrichum acutatum; Andean lupin; polymerase chain reaction; anthracnose; identification; seed

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Agronomy
• Rocznik: 2005
• Tom: 08
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/volume8/issue1/art-02.html

Twórcy

autor

Kulik T.

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 8, 10-719 Olsztyn, Poland

autor

Pszczolkowska A.

autor

Olszewski J.

autor

Fordonski G.

autor

Plodzien K.

autor

Sawicka-Sienkiewicz E.

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