New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures

• Autorzy: El T. A. , Boisbrun M. , Ptak A. , Dupire F. , Chretien F. , Henry M. , Chapleur Y. , Laurain-Mattar D.
• Języki publikacji: EN

Abstrakty

EN

 Biotransformation of deuterated-4'-O-methylnorbelladine into alkaloids galanthamine and lycorine in tissue cultures of Leucojum aestivum was demonstrated using HPLC coupled to mass spectrometry. GC-MS screening was also carried to investigate other native and deuterated alkaloids. A total of six labeled alkaloids were identified indicating that 4'-O-methyl-d3-norbelladine is incorporated into three different groups of Amaryllidaceae alkaloids that are biosynthesized by three modes of intramolecular oxidative phenol coupling.

Słowa kluczowe

EN

Amaryllidaceae; alkaloid; biosynthesis; biotransformation; deuterium-labeled precursor; tissue culture; Leucojum aestivum; pharmacological property

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 1
• Opis fizyczny: p.75-82,fig.,ref.

Twórcy

autor

El T. A.

• UMR 7565 CNRS-Nancy-Universite, Nancy-Vandoeuvre, France

autor

Boisbrun M.

autor

Ptak A.

autor

Dupire F.

autor

Chretien F.

autor

Henry M.

autor

Chapleur Y.

autor

Laurain-Mattar D.

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