TEM and SEM observations on the extracellular matrix of the developing murine centra

• Autorzy: Meyer W , Ramspott M. , Sauerbier I. , Jackowiak H. , Godynicki S.
• Języki publikacji: EN

Abstrakty

EN

TEM and SEM application demonstrated that the shift from chondrification to ossification in the developing murine centra from day 15 to day 18 of gestational age is marked by typical structural variations of the extracellular matrix (ECM). During day 15 GA, typical matrix vesicles with crystalline contents appeared, as followed by single and fusing pleomorphic aggregates of a more regular crystalline structure. During days 17/18 GA, these structures disappeared, and the ECM now exhibited a network of collagen fibrils that had been less conspicuous before. During the time period studied, the ECM switched from a more acid (proteoglycans) to a rather neutral (glycoproteins) milieu.

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2001
• Tom: 60
• Numer: 3
• Opis fizyczny: p.181-189,fig.,ref.

Twórcy

autor

Meyer W

• School of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany

autor

Ramspott M.

autor

Sauerbier I.

autor

Jackowiak H.

autor

Godynicki S.

Bibliografia

• 1. Ali SY (1976) Analysis of matrix vesicles and their role in the calcification of epiphyseal cartilage. Fed Proc, 35: 135–142.
• 2. Anderson HC (1989) Biology of disease: Mechanism of mineral formation in bone. Lab Invest, 60: 320–330.
• 3. Ben Hur H, Ornoy A (1984) Ultrastructural studies of initial stages of mineralization of long bones and vertebrae in human fetuses. Acta Anat, 119: 33–39.
• 4. Boskey A (1999) Mineralization, structure, and function of bone. In: Seibel MJ, Robins SP, Bilezikian JP (eds.). Dynamics of Bone and Cartilage Metabolism. Academic Press, San Diego – Toronto, pp. 153–164.
• 5. Chandraraj S, Briggs CA (1988) Role of cartilage canals in osteogenesis and growth of the vertebral centra. J Anat, 158: 121–136.
• 6. Christ B, Wilting J (1992) From somites to vertebral column. Ann Anat, 174: 23–32.
• 7. Crissman RS, Low FN (1974) A study of fine structural changes in the cartilage-to-bone transition within developing chick vertebra. Am J Anat, 140: 451–470.
• 8. Furumoto TA, Miura N, Akasaka T, Mizutani-Kosecki Y, Sudo H, Fukuda K, Maekawa M, Yuasa S, Fu Y, Moriya H, Taniguchi M, Imai K, Dahl E, Balling R, Pavlova M, Gossler A, Kosecki H (1999) Notochord-dependent expression of MFH1 and PAX1 cooperates to maintain the proliferation of sclerotome cells during vertebral column development. Dev Biol, 210: 15–29.
• 9. Glaser JH, Conrad HE (1981) Formation of matrix vesicles by cultured chick embryo chondrocytes. J Biol Chem, 256: 12607–12611.
• 10. Hale JE, Wuthier RE (1987) The mechanism of matrix vesicle formation. J Biol Chem, 262: 1916–1925.
• 11. Hosokawa R, Uchida Y, Fujiwara S, Noguchi T (1988) Lactate dehydrogenase isoenzymes are present in matrix vesicles. J Biol Chem, 263: 10045–10047.
• 12. Kanabe S, Hsu HHT, Cecil RNA, Anderson HC (1983) Electron microscopic localization of adenosine triphosphatase (ATP)-hydrolysing activity in isolated matrix vesicles and reconstituted vesicles from calf cartilage. J Histochem Cytochem, 31: 462–470.
• 13. Karnovsky MJ (1971) Use of ferrocyanide reduced osmium tetroxide in electron microscopy. Am Soc Cell Biol, 11th Meet, pp. 146.
• 14. LeDouarin NM, Dupin E (1993) Cell lineage analysis in neural crest ontogeny. J Neurobiol, 24: 146–161.
• 15. Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol, 9: 409–414.
• 16. Monsoro-Burq AH, LeDouarin NM (2000) Duality of molecular signaling involved in vertebral chondrogenesis. Curr Top Dev Biol, 48: 43–75.
• 17. Mowry RW (1963) The special value of methods that colour with acidic and vicinal hydroxyl groups in the histochemical study of mucus. With revised directions for the colloidal iron stain, the use of Alcian blue 8 GX and their combination with the periodic acid-Schiff reaction. Ann NY Acad Sci, 106: 402–423.
• 18. Mwale F, Billinghurst C, Wu W, Alini M, Webber C, Reiner A, Ionescu M, Poole J, Poole AR (2000) Selective assembly and remodelling of collagens II and IX associated with expression of the chondrocyte hypertrophic phenotype. Dev Biol 218: 648–662.
• 19. Peress NS, Anderson HC, Sajdera SW (1974) The lipids of matrix vesicles from bovine fetal epiphyseal cartilage. Calcif Tiss Res, 14: 275–281.
• 20. Reinholt FP, Engfeldt B, Hjerpe A, Jansson K (1982) Stereological studies on the epiphyseal growth plate with special reference to the distribution of matrix vesicles. J Ultrastruct Res, 80: 270–279.
• 21. Rugh R (1990) The Mouse. Its Reproduction and Development. Oxford University Press, Oxford.
• 22. Siegel SA, Hummel CF, Carty RP (1983) The role of nucleoside triphosphate pyrophosphohydrolase in in vitro nucleoside triphosphate-dependent matrix vesicle calcification. J Biol Chem, 258: 1128–1135.
• 23. Töndury G, Theiler K (1990) Entwicklungsgeschichte und Fehlbildungen der Wirbelsäule. Hippokrates, Stuttgart.