Cytokine mRNA expression and IFN-gamma production of immunised nematode resistant and susceptible lambs against natural poly-generic challenge

• Autorzy: Pernthaner A , Cabaj W , Stankiewicz M , Davies J , Maass D
• Języki publikacji: EN

Abstrakty

EN

IL-2, IL-4 and IFN-γ mRNA expression, and production of IFN-γ was examined in mesenteric lymph node cells (MLNC) and CD4⁺ enriched T cell populations of nematode resistant (R) and susceptible (S) line lambs by use of RT-PCR and ELISA. Five R and S line lambs that were immunised by repeated oxfendazole-abbreviated infections and 5 non-immunised R and S line lambs were used. All lambs grazed nematode infected pasture for 107 days. Immunisation enhanced the resistant status in both R and S lambs. MLNC obtained from slaughtered animals were stimulated with Con A or T. colubriformis specific antigen. Non-stimulated MLNC of immunised lambs expressed higher levels of IL-4 mRNA and lower levels of IL-2 mRNA than non-immunised lambs. MLNC of immunised R and S line lambs stimulated with antigen for 24 h expressed detectable amounts of IL-4 mRNA that was not seen in non-immunised controls. CD4⁺ T cell enriched cell populations of immunised R and S lambs and non-immunised R lambs expressed moderate to high levels of IL-4 mRNA. Con A stimulated MLNC of immunised R and S lambs expressed high levels IFN-γ mRNA and produced high amounts of IFN-γ. Lower levels were present in non-immunised controls. The results indicate that R line lambs and immunised S line lambs respond to natural nematode challenge with a predominating IL-4 cytokine response when compared to non-immunised S lambs.

Słowa kluczowe

EN

infection; purification; mesenteric lymph node cell; mRNA expression; cell culture; parasite; antigen; susceptible lamb; RNA extraction; cytokine; nematode; lamb; cDNA synthesis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1997
• Tom: 42
• Numer: 3
• Opis fizyczny: p.180-186

Twórcy

autor

Pernthaner A

• Wallaceville Animal Research Centre, P.O.Box 40063, Upper Hutt, New Zealand
• II. Med. Clinic, Veterinary University Vienna, Josef-Baumann-Gasse 1, A-1210 Vienna, Austria

autor

Cabaj W

• W. Stefański Institute of Parasitology of Polish Academy of Sciences, Twarda 51/55, 00-818 Warszawa, Poland

autor

Stankiewicz M

• Wallaceville Animal Research Centre, P.O.Box 40063, Upper Hutt, New Zealand

autor

Davies J

• Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 3168, Australia

autor

Maass D

• Wallaceville Animal Research Centre, P.O.Box 40063, Upper Hutt, New Zealand

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