DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver

• Autorzy: Ferraro A. , Eufemi M. , Cervoni L. , Altieri F. , Turano C.
• Języki publikacji: EN

Abstrakty

EN

To detect the interactions of DNA with the nuclear matrix proteins, DNA-protein cross-linkages were induced in intact nuclei from chicken liver by the use of ds-diammine dichloroplatinum. Methods have been devised for fast purification both of the proteins and of the DNA fragments involved in the cross-linked complexes. By Southern-Western blotting a number of matrix proteins isolated from the complexes have been shown to recognize specifically DNA sequences present in the cross-linked DNA fragments. This experimental approach not only allows to identify the nuclear matrix-DNA interactions existing in the nucleus before its disruption, but also provides a preparation of matrix proteins enriched in those species which are involved in such interactions and which can therefore be detected with high sensitivity.

Słowa kluczowe

EN

avian liver; cis-diamminedichloroplatinum; cross-linking reaction; DNA binding; protein; nuclear matrix; protein purification; DNA purification

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.145-151,fig.

Twórcy

autor

Ferraro A.

• University la Sapienza, Rome, Italy

autor

Eufemi M.

autor

Cervoni L.

autor

Altieri F.

autor

Turano C.

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