Experimental models of cyclosporine A nephrotoxicity

• Autorzy: Korolczuk A , Maciejewski M. , Czechowska G. , Celinski K. , Korobowicz E.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to compare the most commonly-used experimental models and to assess the microscopic renal changes in different models of cyclosporine A (CsA) nephrotoxicity. Wistar male rats were divided into five groups, eight animals in each. CsA was given in doses of 15 mg/kg, 25 mg/kg, and 100 mg/kg, respectively. The blood was collected for creatinine, urea, and uric acid levels analysis in the serum and the kidneys were sampled for microscopic examination on the 11th and 29th d of the experiment. CsA induced nephrotoxicity was characterised by increased serum levels of creatinine, urea, and uric acid. Microscopic features of CsA nephrotoxicity in all CsA experimental groups were observed. We would recommend the use of low doses of CsA for approximately 28 d as the most relevant experimental procedure for achieving the features of chronic CsA nephrotoxicity.

Słowa kluczowe

EN

rat; cyclosporin A; nephrotoxicity; experimental model; Wistar rat; male

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 1
• Opis fizyczny: p.59-62,fig.,ref.

Twórcy

autor

Korolczuk A

• Medical University, 20-950 Lublin, Poland

autor

Maciejewski M.

autor

Czechowska G.

autor

Celinski K.

autor

Korobowicz E.

Bibliografia

• 1. Bobadilla N.A., Gamba G., Tapia E.: Role of NO in cyclosporine nephrotoxicity: effects on chronic NO inhibition and NO synthases gene expression. Am J Physiol 1998, 274, 791-798.
• 2. Bobadilla N., Gamba G.: New insights into the pathophysiology of cyclosporine nephrotoxicity: a role of aldosterone. Am J Renal Physiol 2007, 293, 2-9.
• 3. Burdmann E., Andoh T., Rosen S., Lindsley J., Munar M., Elxinga W., Bennet W.: Experimental nephrotoxicity, hepatotoxicity and pharmacokinetics of cyclosporin G versus cyclosporin A. Kidney Int 1994, 45, 684-691.
• 4. Capasso G., Di Gennaro C., Ragione F., Manna K., Ciarcia R., Florio S., Perna A., Pollastro R., Damiano S., Mazzoni O., Galletti P., Zappia V.: In vivo effect of the natural antioxidant hydroxytyrosol on cyclosporine nephrotoxicity in rats. Nephrol Dial Transplant 2008, 23, 1186-1195.
• 5. Feria I., Pichardo I., Juarez P., Ramirez V., Gonzalez M.A., Uribe N., Garcia-Torres R., Lopez-Casillas F., Gambra G., Bobadilla N.: Terapeutic benefits of spironolactone in experimental chronic cyclosporine nephrotoxicity. Kidney Int 2003, 63, 43-52.
• 6. Jackson N.M., Hsu C.-H., Vissher G.E.: Alterations in renal structure and function in a rat model of cyclosporine nephrotoxicity. J Pharmacol Exp Ther 1987, 242, 749-756.
• 7. McNally P.G., Feehally J.: Pathophysiology of cyclosporin A nephrotoxicity: experimental and clinical observations. Nephrol Dial Transplant 1992, 7, 791-804.
• 8. Mihatch M.J., Ryffel B., Hermle M.: Morphology of cyclosporine nephrotoxicity in the rat. Clin Nephrol 1986, 25, 2-8.
• 9. Pallet N., Rabant M., Xu-Dubois Y., LeCorre D., Mucchielli M-H., Imbeaud S., Agier N., Hertig A., Thervet E., Legendre C., Baune P., Anglicheau D.: Response of human renal tubular cells to cyclosporine and sirolimus: a toxicogenomic study. Toxicol Appl Pharmacol 2008, 229, 184-196.
• 10. Thliveris J., Yatscoff R., Lukowski M., Copeland K.: Cyclosporine nephrotoxicity-experimental models. Clin Biochem 1991, 24, 93-95.
• 11. Wongmekiat O., Leelarugrayub N., Thamprasert K.: Beneficial effect of shallot (Allium ascalonicum L.) extract on cyclosporine nephrotoxicity in rats. Food Chem Toxicol 2008, 46, 1844-1850.
• 12. Wissmann C., Frey F., Ferrari P., Uehlinger D.: Acute cyclosporine-induced nephrotoxicity in renal transplant recipients: the role of the transplanted kidney. J Am Soc Nephrol 1996, 7, 2677-2681.
• 13. Uz E., Bayrak O., Uz E., Kaya E., Bayrak A., Uz B., Turgut F.H., Bavbek N., Kanbay M., Akcay A.: Nigella sativa oil for prevention of chronic cycloxporine nephrotoxicity: an experimental model. Am J Nephrol 2008, 28, 517-522.
• 14. Young B.A., Burdmann E.A., Johnson R.J., Andoh T., Bennett W.M., Couser W.G., Alpers C.E.: Cyclosporine A induced arteriolopathy in a rat model of chronic cyclosporine nephropathy. Kidney Int 1995, 45, 431-438.