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Czasopismo

2005 | 64 | 4 |

Tytuł artykułu

An electron microscopic study of the phosphatases in the ciliate Balantidium coli

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The localisation and activity of D glucose-6-phosphatase (G-6-Pase) and alkaline phosphatase (AlP) in the trophozoites of Balantidium coli isolated from pig intestine content were investigated using ultrastructural and cytochemical methods. The activity of G-6-Pase was demonstrated on the membranes of the endoplasmic reticulum, particularly in the cortical part of the trophozoites. In addition, the product of the reaction to G-6-Pase was concentrated in the vesicular structures, which were distributed along the reticular membranes. These structures were described as vesicles similar to glycosomes, containing enzymes of glycogenolysis. It is very likely that hydrolases in B. coli are formed on the rough reticular membranes without the involvement of cisterns of the Golgi complex. The ultrastructural deposits of the reaction to G-6-Pase and AlP in the trophozoites of B. coli described here indicate that some membranes of the rough endoplasmic reticulum and small vacuoles with a strong reaction to these enzymes can play a similar role to the Golgi complex.

Wydawca

-

Czasopismo

Rocznik

Tom

64

Numer

4

Opis fizyczny

p.282-286,fig.,ref.

Twórcy

autor
  • Szczecin University, Al.Piastow 40B, 71-215 Szczecin, Poland

Bibliografia

  • 1. De Jong A, Van Dujin P, Daems W (1976) Cytochemical model system for microsomal rat liver glucose-6-phosphatase. J Histochem Cytochem, 24: 643–651.
  • 2. Grim JM, Clements KD, Byfield T (2002) New Species of Balantidium and Paracichlidotherus (Ciliophora) inhabiting the intestines of four surgeonfish species from the Tuvalu Islands, Pacific Ocean. J Eukaryot Microbioll, 2: 146–153.
  • 3. Hryniewiecka-Szyfter Z, Kuźna-Grygiel W (1970) Histochemical localization of acid and alkaline phosphatases in throphozoites and cysts of amoeba Hartmanella castellanii Neff Bull Soc Sc Lettr (Poznań), 11: 31–38.
  • 4. Humiczewska M, Skotarczak B (1984) The influence of Balantidium coli (Malmsten) on liver’s morphology and metabolism of Mesocricetus auratus (Waterhouse). Wiad Parazytol, 5–6: 575–583.
  • 5. Kierek-Jaszczuk D (1981) Heterogeneity of mammalian alkaline phosphatase (in Polish). Post Biochem, 27: 217–264.
  • 6. Klumpp S, Schultz JE (1990) Alkaline phosphatase from Paramecium cilia and cell bodies: purification and characterization. Biochim Biophysis Acta, 1037: 233–239.
  • 7. Krygier-Stojałowska A, Godlewski H (1982) Topochemiczne metody badań komórek i tkanek. Państwowe Wydawnictwo Naukowe, Warszawa, pp. 628–648.
  • 8. Kuźna-Grygiel W (1994) Ultrastructural localization of enzymes in Entamoeba histolytica. I. Acid phosphatase, alkaline phosphatase and thiamine pyrophosphatae. Acta Protozool, 33: 233–238.
  • 9. Lazarov PB, Fujiki Y (1989) Biogenesis of peroxisomes. Ann Rev Cell Biol, 1: 489–530.
  • 10. Lumpert CJ, Kersken H, Gras U, Plattner H (1987) Screening of enzymatic mechanisms possibly involved in membrane fusion during exocytosis in Paramecium cells. Cell Biol Int Rep, 11: 405–414.
  • 11. Opperdoes FR (1988) Glycosomes may provide clues to the import of peroxisomal proteins. TIBS, 13: 255–260.
  • 12. Pearse AGE (1972) Histochemistry: theoretical and applied, vol. II, Churchill Livingstone, Edinburgh, pp. 808–826.
  • 13. Skotarczak B (1980) Activity of phosphatases in Balantidium coli. I. Folia Biol (Kraków), 28: 63–67.
  • 14. Skotarczak B (1986) Cyto- and histochemical studies on the enzymes activity in Balantidium coli (M) and Mesocricetus auratus (W) in experimental balantidiosis. I. Phosphatases. Acta Parasitol Pol, 29: 275–288.
  • 15. Skotarczak B, Leonaldi R (1986) Localizzazione ultrastrutturale della fosfatasi acida in Balantidium coli (Malmsten). Esperienze di Medicina e Chirurgia, 2: 109–118.
  • 16. Skotarczak B (1997) Bacterial flora in acute and symptom-free balantidiosis. Acta Parasitol, 42: 230–233.
  • 17. Skotarczak B (1997) Ultrastructural and cytochemical identification of peroxisomes in Balantidium coli, Ciliophora. Folia Biol (Kraków), 45: 117–120.
  • 18. Skotarczak B (1997) Ultrastructure of the nucleus system of Balantidium coli. J Protozool Res, 7: 127–132.
  • 19. Skotarczak B (1999) Cytochemical identification of mucocysts in Balantidium coli trophozoites. Folia Biol (Kraków), 47: 61–65.
  • 20. Skotarczak B (1999) The formation of primary and secondary lysosomes in Balantidium coli, Ciliata. Folia Histochem Cytobiol, 37: 261–265.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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