An ultrastructural demonstration of NADPH-diaphorase-nitric oxide synthase activity in the rat striatal astroglia

• Autorzy: Calka J , Wolf G.
• Języki publikacji: EN

Abstrakty

EN

In the present study we have used electron microscopical NADPH-diaphorase (NADPH-d) histochemistry as a visualization procedure for nitric oxide synthase (NOS) to examine patterns of activity in the subcellular distribution of NADPH-d in the rat striatal astroglia. Electron microscopical examination revealed deposition of the NADPH-d reaction product in a nuclear envelope, fragments of endoplasmic reticulum and mitochondria. Predominantly mitochondria of astrocytic “end feet” were labelled. Our ultrastructural observations promote the possibility that astroglial NADPH-d/NOS is involved in adaptation of the local blood flow in the striatal microenvironment.

Słowa kluczowe

EN

ultrastructure; NADPH-diaphorase; nitric oxide synthase; rat; striatal astroglia; astroglia

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2003
• Tom: 62
• Numer: 3
• Opis fizyczny: p.251-253,fig.,ref.

Twórcy

autor

Calka J

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

autor

Wolf G.

Bibliografia

• 1. Całka J, Schmidt W, Wolf G (1996) Induction of cytosolic NADPH-diaphorase/nitric oxide synthase in reactive microglia/macrophages after quinolinic acid lesions in the rat striatum: an electron and light microscopical study. Histochem Cell Biol, 105: 81–89.
• 2. Iadecola C (1993) Regulation of the cerebral microcirculation during neural activity: is nitric oxide the missing link? TINS, 16: 206–216.
• 3. Kobari M, Fukuuchi Y, Tomita M, Tanahashi N, Takeda H (1994) Role of nitric oxide in regulation of cerebral microvascular tone and autoregulation of cerebral blood flow in cats. Brain Res, 667: 255–262.
• 4. Lee SC, Dickson DW, Liu W, Brosnan CF (1993) Induction of nitric oxide synthase activity in human astrocytes by interleukin-1ß and interferon. J Neuroimunol, 46: 19–24.
• 5. Tracey WR, Nakane M, Pollock JS, Forstermann U (1993) Nitric oxide synthase in neuronal cells, macrophages and endothelium are NADPH diaphorases, but represent only a fraction of total cellular NADPH-diaphorase activity. Biochem Biophys Res Commun, 195: 1035–1040.
• 6. Paakkari I, Lindsberg P (1995) Nitric oxide in the central nervous system. Ann Med, 27: 369–377.