Effects of thapsigargin on stimulation frequency - dependent changes in mitochondrial calcium in rat cardiac myocytes

• Autorzy: Pytkowski B. , Lewartowski B.
• Języki publikacji: EN

Abstrakty

EN

We investigated in the single myocytes of rat heart the effect of blocking of ATP-ase of sarcoplasmic reticulum (SR) on mitochondrial Ca2+ uptake and release. Mitochondrial Ca2+ content was investigated as Mn2+ - resistant fluorescence of Indo 1 - AM loaded into cells. SR ATP-ase was blocked with 10-6 M thapsigargin (Tg). Tg blocked almost completely stimulation Œdependent mitochondrial Ca2+ uptake and slowed down its release despite that the maximal cytosolic Ca2+ concentration was not decreased. We propose that mitochondrial stimulation -dependent Ca2+ uptake is greatly enhanced by [Ca2+ ] built by SR in microdomains adjacent to these organelle.

Słowa kluczowe

EN

sarcoplasmic reticulum; cardiac myocyte; calcium; mitochondrion; ATPase; rat; thapsigargin

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2002
• Tom: 53
• Numer: 4,2
• Opis fizyczny: p.751-759,fig.

Twórcy

autor

Pytkowski B.

• Medical Center of Postgraduate Education, Marymoncka 99, 01-813 Warsaw, Poland

autor

Lewartowski B.