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2005 | 46 | 1 |

Tytuł artykułu

DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The diacylglycerol o-acyltransferase 1 gene (OGAT1) was investigated in Polish Black-and-White cattle. The frequency of the K allele was 0.60, 0.68 and 0.48 for AI sires (n = 150), young bulls (n = 139) and cows (n = 213), respectively. The method of selective genotyping for identification of the quantitative trait nucleotide was verified through identification of DGAT1 effect on milk production traits. Daughters of six heterozygous bulls were selectively genotyped based on their milk traits. The genotypic frequencies differed between high and low yield groups representing milk and fat contents. The Kruskal-Wallis test revealed a highly significant effect of DGAT1 K232A in cows with extremely low fat content and a significant effect in cows with extremely high protein content of milk. No significant effect of AI sires’ genotypes on their breeding value was found.

Wydawca

-

Rocznik

Tom

46

Numer

1

Opis fizyczny

p.85-87,ref.

Twórcy

autor
  • Department of Animal Genetics, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Department of Animal Genetics, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Department of Animal Genetics, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Department of Animal Science, Terrill Hall, University of Vermont, Burlington, USA
autor
  • Department of Animal Genetics, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn, Poland

Bibliografia

  • Bennewitz J, Reinsch N, Paul S, Looft C, Kaupe B, Weimann C, et al. 2004. The DGAT1 K232A mutation is not solely responsible for the milk production quantitative trait locus on the bovine chromosome 14. J Dairy Sci 87:431-442.
  • Cases S, Smith SJ, Zheng Y, Myers HM, Lear SR, Sande E, et al. 1998. Identification of a gene encoding an acyl CoA: diacylglycérol acyltransferase, a key enzyme in triglycerol synthesis. Proc Natl Acad Sci USA: 95: 13018-13023.
  • Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS, 1991. ‘Touch down’ PCR to circumvent spurious priming during gene amplification. Nuc Acid Res 19: 4008.
  • Fisher PJ, Spelman RJ, 2004. Verification of selective DNA pooling methodology through identification and estimation of the DGAT1 effect. Anim Genet 35: 201-205.
  • Georges M, Nielsen D, Mackinnon M, Mishra A, Okimoto R, Pasquino AT, et al. 1995. Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing. Genet 139: 907-920.
  • Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Cambisano N, et al. 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genom Res 12: 222-231.
  • Grisart B, Farnir F, Karim L, Cambisano N, Kim J, Kvasz Kasz, et al. 2004. Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition. Proc Natl Acad Sci USA: 101:2398-2403.
  • Kaupe B, Winter A, Jann OC, Ibeagha EM, Ozbeyaz C, Williams JL, et al. 2002. Screening of Bos taurus and Bos indicus cattle for DGAT1 polymorphism. Proceed ISAG, Gottingen: D162.
  • Kühn C, Thaller G, Winter A, Bininda-Emonds ORP, Kaupe B, Erhardt G, et al. 2004. Evidence for multiple alleles at the DGAT1 locus better explains a quantitative trait locus with major effect on milk fat content in cattle. Genet 167: 1873-1881.
  • Pareek CS, Pareek RS, Walawski K, 2002a. Novel linkage mapping approach using DNA pooling in human and animal genetics I. Detection of complex disease loci. J Appl Genet 43: 175-192.
  • Pareek CS, Pareek RS, Walawski K, 2002b. Novel linkage mapping approach using selective DNA pooling in human and animal genetics. II. Detection of quantitative traits loci in dairy cattle. J Appl Genet 43: 309-318.
  • Spelman RJ, Ford CA, McElhinney P, Gregory GC, Snell RG, 2002. Characterization of the DGAT1 gene in the New Zealand dairy population J Dairy Sci 85: 3514-3517.
  • Thaller G, Krämer W, Winter A, Kaupe B, Erhardt G, Fries R, 2003. Effects of DGAT1 variants on milk production traits in German cattle breeds. J Anim Sci 81: 1911-1918.
  • Weller JI, Golik M, Seroussi E, Ezra E, Ron M, 2003. Population-wide analysis of a QTL affecting milk-fat production in the Israeli Holstein population. J Dairy Sci 86: 2219-2227.
  • Winter A, Krämer W, Werner FA, Kollers S, Kata SR, Durstewitz G, et al. 2002. Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc Natl Acad Sci USA: 99: 9300-9305.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-76c6a3b5-029e-46d8-be2d-e32547130001
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