Activation of the intrinsic and extrinsic pathways in high pressure-induced apoptosis of murine erythroleukemia cells

• Autorzy: Yamaguchi T , Hashiguchi K. , Katsuki S. , Iwamoto W. , Tsuruhara S. , Terada S.
• Języki publikacji: EN

Abstrakty

EN

We previously demonstrated that caspase-3, an executioner of apoptosis, is activated in the pressure-induced apoptosis of murine erythroleukemia (MEL) cells (at 100 MPa). Here, we examined the pathway of caspase-3 activation using peptide substrates and caspase inhibitors. Using the substrates of caspases-8 and -9, it was found that both are activated in cells under high pressure. The production of nuclei with sub-G1 DNA content in 100 MPa-treated MEL cells was suppressed by inhibitors of caspases-8 and -9, and pan-caspase. In 100 MPa-treated cells, pan-caspase inhibitor partially prevented the cytochrome c release from the mitochondria and the breakdown of mitochondrial membrane potential. These results suggest that the intrinsic and extrinsic pathways are activated in apoptotic signaling during the high pressure-induced death of MEL cells.

Słowa kluczowe

EN

apoptosis; caspase; cytochrome C; flow cytometry; membrane potential; high pressure; intrinsic patchway; extrinsic patchway; murine cell; erythroleukemia cell

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 1
• Opis fizyczny: p.49-57,fig.,ref.

Twórcy

autor

Yamaguchi T

• Fukuoka University, Jonan-ku, Fukuoka, 814-0180, Japan

autor

Hashiguchi K.

autor

Katsuki S.

autor

Iwamoto W.

autor

Tsuruhara S.

autor

Terada S.

Bibliografia

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