Peroksydacja lipidow w narzadach szczurow przy ostrym zapaleniu trzustki

• Autorzy: Barham S , Truchlinski J. , Ognik K.

Warianty tytułu

EN

Peroxidation of lipids in rats' organs during acute pancreatitis

• Języki publikacji: PL

Abstrakty

EN

The experiment was carried out using 32 Wistar strain male rats of 280-320 g body weight. The animals were randomly divided into 2 groups. Control group I were healthy animals (8 rats). In group II, acute pancreatitis was induced by injecting 5% sodium taurocholate solution into the common bile-pancreas duct. Twenty-four hours before the operation, animals were food deprived and were given Dormicum (from a vial) in ad libitum drinking water. After acute pancreatitis induction, the animals of group II were additionally divided into 3 sub-groups: A, B, and C according to the time the disease had developed. Blood and internal organs from sub-group A were sampled after 3 hours, from group B after 24 hours, and from group C - after 48 hours. Organs were homogenized in 20 mM Tris buffer at pH 7.4. Products of lipid peroxidation were determined in supernatant: conjugated dienes, hydroperoxilipid dienes and malonic aldehyde. Total anti-oxidation potential was determined in blood serum along with its components: uric acid, urea and bilirubin. a-amylase in serum activity was also recorded to confirm the development of acute pancreatitis. The results of the study revealed very variable dynamics of lipid peroxidation in organs essential for survival during acute pancreatitis. This suggests that the mechanisms of anti-oxidation defense are not able to successfully protect the organism against oxidation stress during acute pancreatitis, which consequently enhances the risk of multi-organ failure syndrome. A detailed explanation of the oxidation stress role in the course of acute pancreatitis and its complications requires further integrated studies at the molecular level, cellular culturing and on animal models.

Słowa kluczowe

PL

szczury; ostre zapalenie trzustki; wydolnosc antyoksydacyjna; peroksydacja lipidow; przeciwutleniacze

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 04
• Opis fizyczny: s.440-443,tab.,bibliogr.

Twórcy

autor

Barham S

• Samodzielny Publiczny Szpital Wojewodzki im.Jana Bozego, ul.Biernackiego 9, 20-089 Lublin

autor

Truchlinski J.

autor

Ognik K.

Bibliografia

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