Interstrain diversity of 2-keto-3-deoxyoctonate content in lipopolysaccharides of Desulfovibrio desulfuricans

• Autorzy: Lodowska J , Wolny D. , Jaworska-Kik M. , Dzierzewicz Z. , Weglarz L.
• Języki publikacji: EN

Abstrakty

EN

Bacteria of Desulfovibrio desulfuricans species are Gram-negative, anaerobic rods selectively reducing sulphates and colonizing oxygen-free ecosystems. They are ubiquitous in the natural environment and have been also found to reside in the human digestive tract. They are suggested to be involved in the pathogenesis of ulcerative colitis and Crohn's disease. The D. desulfuricans wild strains were isolated from feces and bioptate of patients suffering from various digestive tract disorders. LPSs were isolated from the wild enteric strains and soil type strain La 2226 of D. desulfuricans and analyzed in terms of their 2-keto-3-deoxyoctulosonic acid (Kdo) component content. The obtained spectrophotometric data indicate that Kdo content is characteristic of each of the investigated strains and it ranges from 0.48% to 2.86% (w/w) of the total LPS mass. Statistically significant interstrain differences of Kdo quantity seem to suggest the differences in the O-antigen content. Comparative analysis of Kdo content in LPSs of D. desulfuricans strains in relation to that of the reference endotoxin from Salmonella spp. allows us to suggest that D. desulfuricans bacteria possess O-antigen polysaccharides composed of diverse number of carbohydrate units.

Słowa kluczowe

EN

man; Crohn's disease; 2-keto-3-deoxyoctulosonic acid; gastrointestinal tract; 2-keto-3-deoxyoctonate; Desulfovibrio desulfuricans; interstrain diversity; lipopolysaccharide; endotoxin

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 1
• Opis fizyczny: p.21-27,fig.,ref.

Twórcy

autor

Lodowska J

• Medical University of Silesia, Narcyzow 1, 41-200 Sosnowiec, Poland

autor

Wolny D.

autor

Jaworska-Kik M.

autor

Dzierzewicz Z.

autor

Weglarz L.

Bibliografia

• Aspinall G.O., A. McDonald, T.S. Raju, H. Pang, L.A. Kurjanczyk, J.L. Penner and A.P. Moran. 1993. Chemical structure of the core region of Campylobacter jejuni serotype O:2 lipopolysaccharide. Eur. J. Biochem. 213: 1029-1037.
• Brade H. and E.T. Rietschel. 1984. α-2->4-interlinked 3-deoxy-D-manno-octulosonic acid disaccharide. A common constituent of enterobacterial lipopolysaccharides. Eur. J. Biochem. 145: 231-236.
• Brade L., S. Schramek, U. Schade and H. Brade. 1986. Chemical, biological, and immunochemical properties of the Chlamydia psittaci lipopolysaccharide. Infect. Immun. 54: 568-574.
• Bradley G. and C.C. Gaylarde. 1988. Iron uptake by Desulfovibrio vulgaris outer membrane components in artificial vesicles. Curr. Microbiol. 17: 189-192.
• Caroff M., S. Lebbar and L. Szabo. 1987. Do endotoxins devoid of 3-deoxy-D-manno-2-octulosonic acid exist? Biochem. Biophys. Res. Commun. 143: 845-847.
• D'Haeze W., C. Leoff, G. Freshour, K.D. Noel and R.W. Carlson. 2007. Rhizobium etli CE3 bacteroid lipopolysaccharides are structurally similar but not identical to those produced by cultured CE3 bacteria. J. Biol. Chem. 282: 17101-17113.
• Dzierżewicz Z., B. Cwalina, M. Jaworska-Kik, L. Węglarz and T. Wilczok. 2001. Susceptibility to antibiotics and biochemical properties of Desulfovibrio desulfuricans strains. Acta Pol. Pharm. -Drug Res. 58: 439-444.
• Dzierżewicz Z., B. Cwalina, S. Kurkiewicz, E. Chodurek and T. Wilczok. 1996. Intraspecies variability of cellular fatty acids among soil and intestinal strains of Desufovibrio desulfuricans. Appl. Environ. Microbiol. 62: 3360-3365.
• Dzierżewicz Z., B. Gawlik, B. Cwalina, K. Gonciarz, G. Ziółkowski, Z. Gonciarz and T. Wilczok. 1994. Activity of sulphate reducing bacteria in the human digestion tract. Bull. Pol. Acad. Sci. 42: 171-176.
• Dzierżewicz Z., A. Orchel, A. Komarska-Szostak, J. Wawszczyk, L. Węglarz, J. Szczerba and T. Wilczok. 2005. Biological activity of endotoxins isolated from Desulfovibrio desulfuricans. Ann. Acad. Med. Siles. 59: 9-16.
• Dzierżewicz Z., J. Szczerba, L. Węglarz, L. Świątkowska, D. Jasińska and T. Wilczok. 2003. Intraspecies variability of Desulfovibrio desulfuricans strains determined by the genetic profiles. FEMS Microbiol. Lett. 219: 69-74.
• Florin T., G. Gibson, G. Neale and J. Cummings. 1990. A role for sulphate-reducing bacteria in ulcerative colitis? Gastroenterology 98: A170.
• Forsberg L.S. and R.W. Carlson. 1998. The structures of the lipopolysaccharides from Rhizobium etli strains CE358 and CE359. The complete structure of the core region of R. etli lipopolysaccharides. J. Biol. Chem. 273: 2747-2757.
• Fukuoka S., Y.A. Knirel, B. Lindner, H. Moll, U. Seydel and U. Zähringer. 1997. Elucidation of the structure of the core region and the complete structure of the R-type lipopolysaccharide of Erwinia carotovora FERM P-7576. Eur. J. Biochem. 250: 55-62.
• Gaylarde C.C. and LB. Beech. 1996. Short Communication: Lipopolysaccharide composition of Desulfovibrio cell wall, World J. Microb. Biot. 12: 113-114.
• Gibson G., J. Cummings and G. MacFarlane. 1991. Growth and activities of sulphate-reducing bacteria in gut contents of healthy subjects and patients with ulcerative colitis. FEMS Microbiol. Ecol. 86: 103-112.
• Haishima Y., O. Holst and H. Brade. 1992. Structural investigation on the lipopolysaccharide of Escherichia coli rough mutant F653 representing the R3 core type. Eur. J. Biochem. 203: 127-134.
• Helander I.M., B. Lindner, H. Brade, K. Altman, A.A. Lindberg, E.T. Rietschel and U. Zähringer. 1988. Chemical structure of the lipopolisaccharide of Haemophilus influrenzae strain 1-69 Rd-/b+. Eur. J. Biochem. 177: 483-492.
• Holst O., W. Broer, J.E. Thomas-Oates, U. Mamat and H. Brade. 1993. Structural analysis of two oligosaccharide bis-phosphates isolated from the lipopolysaccharide of a recombinant strain of Escherichia coli F515 (Re chemotype) expressing the genus-specific epitope of Chlamydia lipopolysaccharide. Eur. J. Biochem. 214: 703-710.
• Holst O., J.E. Thomas-Oates and H. Brade. 1994. Preparation and structural analysis of oligosaccharide monophosphates obtained from the lipopolysaccharide of recombinant strains of Salmonella Minnesota and Escherichia coli expressing the genus-specific epitope of Chlamydia lipopolysaccharide. Eur. J. Biochem. 222: 183-194.
• Jansson P.E., A.A. Lindberg, B. Lindberg and R. Wollin. 1981. Structural studies on the hexose region of the core in lipopolysaccharides from Enterobacteriaceae. Eur. J. Biochem. 115: 571-577.
• Johnson K.G. and M.B. Perry. 1976. Improved techniques for the preparation of bacterial lipopolisaccharides. Can. J. Microbiol. 22: 29-34.
• Karkhanis Y.D., J.Y. Zeltner, J.J. Jackson and D.J. Carlo. 1978. A new and improved microassay to determine 2-Keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal. Biochem. 85: 595-601.
• Katzenellenbogen E. and E. Romanowska. 1980. Structural studies on Shigella flexneri serotype 6 core region. Eur. J. Biochem. 113: 205-211.
• Kiang J., S.C. Szu, L. Wang, M. Tang and Y.C. Lee. 1997. Determination of 2-Keto-3-deoxyoctulosonic Acid (KDO) with High-Performance Anion-Exchange Chromatography (HPAEC): Survey of Stability of KDO and Optimal Hydrolytic Conditions. Anal. Biochem. 245: 97-101.
• Knirel Y.A., H. Moll H. and V. Zähringer. 1996. Structural study of a highly O-acetylated core of Legionella pneumophila serogroup 1 lipopolysaccharide. Carbohydr. Res. 293: 223-234.
• Kondo S., U. Zähringer, U. Seydel, V. Sinnwell, K. Hisatsune and E.T. Rietschel. 1991. Chemical structure of the carbohydrate backbone of Vibrio parahaemolyticus serotype 012 lipopolysaccharide. Eur. J. Biochem. 200: 689-698.
• Lee Ch. and Ch. Tsai. 1999. Quantification of Bacterial Lipopolysaccharides by the Purpald Assay: Measuring Formaldehyde Generated from 2-keto-3-deoxyoctonate and Heptose at the Inner Core by Periodate Oxidation. Anal. Biochem. 267: 161-168.
• Lodowska J., D. Wolny, M. Jaworska-Kik, L. Węglarz, Z. Dzierżewicz and T. Wilczok. 2003. Composition of the polysaccharide component of the endotoxin from the intestinal strain of Desulfovibrio desulfuricans. Ann. Pol. Chem. Soc. 2: 299-303 Preliminary Reports Presented During 46 Meeting of the Polish Chemical Society and the Association of Engineers & Technicians of Chemical Industry, Lublin 15-18.09.2003.
• Lozniewski A., P. Maurer, H. Schuhmacher, J.P. Carlier and F. Mory. 1999. First isolation of Desulfovibrio species as part of a polymicrobial infection from a brain abscess. Eur. J. Clin. Microbiol. Infect. Dis. 18: 602-603.
• Postgate J.R. 1984. The sulphate-reducing bacteria. Cambridge University Press, Cambrige
• Radziejewska-Lebrecht J., A.S. Shashkov, V. Stroobant, K. Wartenbcrg, C. Warth and H. Mayer. 1994. The inner core region of Yersinia enterocolitica Ye 75R(0:3) lipopolisaccharide. Eur. J. Biochem. 221: 343-351.
• Romanowska E., A. Gamian, C. Ługowski, A. Romanowska, J. Dąbrowski, M. Hauck, H.J. Opferkuch and C.W. von der Lieth. 1988. Structure elucidation of the core regions from Citrobacter O4 and O36 lipopolysaccharides by chemical and enzymatic methods, gas chromatography/mass spectrometry, and NMR spectroscopy at 500 MHz. Biochemistry. 27: 4153-4161.
• Rybka J. and A. Gamian. 2006. Determination of endotoxin by the measurement of the acetylated methyl glycoside derivative of Kdo with gas-liquid chromatography-mass spectrometry. J. Microbiol. Methods. 64: 171-184.
• Severn W.B., R.F. Kelly, J.C. Richards and C. Whitfield. 1996. Structure of the core oligosaccharide in the serotype O8 lipopolysaccharide from Klebsiella pneumoniae. J. Bacteriol. 178: 1731-1741.
• Sidorczyk Z., W. Kaca, H. Brade, E.T. Rietschel, V. Sinnwell and U. Zähringer. 1987. Isolation and structural characterization of an 8-O-(4-amino-4-deoxy-beta-L-arabinopyranosyl)-3-deoxy-D-munno-octulosonic acid disaccharide in the lipopolysaccharide of a Proteus mirabilis deep rough mutant. Eur. J. Biochem. 168: 269-273.
• Süsskind M., L. Brade, H. Brade and O. Holst. 1998. Identification of a novel heptoglycan of α(1->2)-linked D-glycero-D-manno-heptopyranose. Chemical and antigenic structure of lipopolysaccharides from Klebsiella pneumoniae ssp. pneumoniae rough strain R20 (O1-K20-). J. Biol. Chem. 273: 7006-7017.
• Tacken A. and H. Brade. 1986. G.L.C-M.S. of partially methylated and acetylated derivatives of 3-deoxyoctitols. Carbohydr. Res. 149: 263-277.
• Tacken A., R.T. Rietschel and H. Brade. 1986. Methylation analysis of the heptose/3-deoxy-D-manno-2-octulosonic acid region (inner core) of the lipopolysaccharide from Salmonella minnesota rough mutants. Carbohydr. Res. 149: 279-291.
• Tee W., M. Dyall-Smith, W. Woods and D. Eisen. 1996. Probable new species of Desulfovibrio isolated from a pyogenic liver abscess. J. Clin. Microbiol. 34: 1760-1764.
• Toman R. and L. Skultety. 1996. Structural study on a lipopolysaccharide from Coxiella burnetii strain Nine Mile in avirulent phase II. Carbohydr. Res. 283:175-185.
• Vinogradov E.V., B.O. Petersen, J.E. Thomas-Oates, J. Duus, H. Brade and O. Hoist. 1998. Characterization of a novel branched tetrasaccharide of 3-deoxy-D-manno-oct-2-ulopyranosonic acid. The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter haumannii strain NCTC 10303 (ATCC 17904). J. Biol. Chem. 273: 28122-28131.