Expression of orexin receptors 1 [OX1R] and 2 [OX2R] in the porcine hypothalamus during the oestrous cycle

• Autorzy: Kaminski T. , Smolinska N. , Nitkiewicz A. , Przala J.
• Języki publikacji: EN

Abstrakty

EN

Orexin A and B are hypothalamic peptides involved in the control of food intake, sleep patterns, autonomic and neuroendocrine systems. The biological actions of orexins are mediated via two G-protein coupled receptors, termed orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). The present study analyzed mRNA and protein expressions of OX1R and OX2R in porcine hypothalamic structures engaged in GnRH production and secretion, preoptic area (POA), mediobasal hypothalamus (MBH), and stalk median eminence (SME) on days 2-3, 10-12, 14-16, and 17-19 of the oestrous cycle. The highest OX1R gene expression in POA was observed on days 17-19 of the cycle. Changes in the mRNA expression in MBH and SME throughout the cycle were negligible. The expression peak of OX2R gene in POA and SME occurred on days 17-19 as well. There were no changes in the gene expression in MBH. OX1R protein content in POA and SME also was the greatest on days 17-19 and OX2R protein expression was most pronounced in MBH and SME during the same phase of the cycle. In conclusion, fluctuation of OX1R and OX2R mRNAs and proteins content in pig hypothalamus dependently on the phase of the oestrous cycle suggests that orexins, through the influence on the hypothalamic-pituitary-ovarian axis, may affect reproductive functions.

Słowa kluczowe

EN

orexin-A; orexin-B; hypothalamic peptide; food intake; automatic system; neuroendocrine system; G-protein coupled receptor; orexin receptor 1; orexin receptor 2; protein expression; mediobasal hypothalamus; hypothalamus; preoptic area; oestrous cycle; gonadotrophin releasing hormone

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 3
• Opis fizyczny: p.363-371,fig.,ref.

Twórcy

autor

Kaminski T.

• University of Warmia and Mazury in Olsztyn, 1A Oczapowski Street, 10-719 Olsztyn, Poland

autor

Smolinska N.

autor

Nitkiewicz A.

autor

Przala J.

Bibliografia

• De Lecea L, Kilduff TS, Peyron C, et al. The hypocretins: hypothalamus-specific peptides with neuroexcitatory activity. Proc Natl Acad Sci USA 1998; 95: 322-327.
• Sakurai T, Amemiya A, Ishii M, et al.. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 1998; 92: 573-585.
• Konturek PC, Konturek JW, Czesnikiewicz-Guzik M, Brzozowski T, Sito E, Konturek SJ. Neuro-hormonal control of food intake; basic mechanisms and clinical implications. J Physiol Pharmacol 2005; 56(Suppl 6): 5-25.
• Kapica M, Pusio I, Kato I, Kuwahara A, Zabielski R. Role of feed-regulating peptides on pancreatic exocrine secretion. J Physiol Pharmacol 2008; 59(Suppl 2): 145-159.
• Spinazzi R, Andreis PG, Rossi GP, Nussdorfer GG. Orexins in the regulation of the hypothalamic-pituitary-adrenal axis. Pharmacol Rev 2006; 58: 46-57.
• Porka-Heiskanen T, Kalinchuk A, Alanko L, Huhtaniemi I, Stenberg D. Orexin A and B levels in the hypothalamus of female rats: the effects of the estrous cycle and age. Eur J Endocrinol 2004; 150: 737-742.
• Barreiro ML, Pineda R, Gaytan F, et al. Pattern of orexin expression and direct biological actions of orexin-A in rat testis. Endocrinology 2005; 146: 5164-5175.
• Silveyra P, Lux-Lantos V, Libertun C. Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: effects of orexin receptor antagonists on gonadotropins and ovulation. Am J Physiol Endocrinol Metab 2007; 293: E977-E985.
• Campbell RE, Grove KL, Smith MS. Gonadotropin-releasing hormone neurons coexpress orexin 1 receptor immunoreactivity and receive direct contacts by orexin fibers. Endocrinology 2003; 144: 1542-1548.
• Su J, Lei Z, Zhang W, Ning H, Ping J. Distribution of orexin B and its relationship with GnRH in the pig hypothalamus. Res Vet Sci 2008; 85: 315-323.
• Small CJ, Goubillon M-L, Murray JF, et al. Central orexin A has site-specific effects on luteinizing hormone release in female rats. Endocrinology 2003; 144: 3225-3236.
• Sasson R, Dearh RK, White RS, Chappell PE, Mellon PL. Orexin A induces GnRH gene expression and secretion from GT1-7 hypothalamic GnRH neurons. Neuroendocrinology 2006; 84: 353-363.
• Russell SH, Small CJ, Kennedy AR, et al. Orexin A interactions in the hypothalamo-pituitary-gonadal axis. Endocrinology 2001; 142: 5294-5302.
• Pu S, Jain MR, Kalra PS, Kalra SP. Orexins, a novel family of hypothalamic neuropeptides, modulate pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Regul Pept 1998; 78: 133-136.
• Furuta M, Funabashi T, Kimura F. Suppressive action of orexin A on pulsatile luteinizing hormone secretion is potentiated be a low dose of estrogen in ovariectomized rats. Neuroendocrinology 2002; 75: 151-157.
• Barb CR, Matteri RL. Orexin-B modulates luteinizing hormone and growth hormone secretion from porcine pituitary cells in culture. Domest Anim Endocrinol 2005; 28: 331-337.
• Johren O, Bruggemann N, Dominiak P. Orexins (hypocretins) and adrenal function. Horm Metab Res 2004; 36: 370-375.
• Samson WK, Taylor MM, Follwell M, Ferguson AV. Orexin actions in hypothalamic paraventricular nucleus: physiological consequences and cellular correlates. Regul Pept 2002; 104: 97-103.
• Kukkonen JP, Holmqvist T, Ammoun S, Akerman KE. Functions of the orexigenic/hypocretinergic system. Am J Physiol Cell Physiol 2002; 283: C1567-C1591.
• Sesti LA, Britt JH. Relationship of secretion of GnRH in vitro to changes in pituitary concentration of LH and FSH and serum concentration of LH during lactation in sows. J Reprod Fertil 1993; 98:393-400.
• Livak KJ, Schmittgen T. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods 2001; 25: 402-408.
• Smolinska N, Siawrys G, Kaminski T, Przala J. Leptin gene and protein expression in the trophoblast and uterine tissues during early pregnancy and the oestrous cycle of pigs. J Physiol Pharamcol 2007; 58: 563-581.
• Silveyra P, Catalano PN, Lux-Lantos V, Libertun C. Impact of proestrous milieu on expression of orexin receptors and prepro-orexin in rat hypothalamus and hypophysis: actions of cetrorelix and nembutal. Am J Physiol Endocrinol Metab 2007; 292: E820-E828.
• Johren O, Neidert SJ, Kummer M, Dendorfer A, Dominiak P. Prepro-orexin and orexin receptor mRNAs are differentially expressed in peripheral tissues of male and female rats. Endocrinology 2001; 142: 3324-3331.
• Johren O, Neidert SJ, Kummer M, Dominiak P. Sexually dimorphic expression of prepro-orexin mRNA in the rat hypothalamus. Peptides 2002; 23: 1177-1180.
• Kanenishi K, Ueno M, Momose S, et al. Prepro-orexin mRNA expression in the rat brain is increased during pregnancy. Neurosci Lett 2004; 368: 73-77.
• Tamura T, Irahara M, Tezuka M, Kiyokawa M, Aono T. Orexins, orexigenic hypothalamic neuropeptides, suppress the pulsatile secretion of luteinizing hormone in ovariectomized female rats. Biochem Biophys Res Commun 1999; 264: 759-762.
• Irahara M, Tamura T, Matuzaki T, et al. Orexin-A suppresses the pulsatile secretion of luteinizing hormone via beta-endorphin. Biochem Biophys Res Commun 2001; 281: 232-236.
• Iwasa T, Matsuzaki T, Kiyokawa M, et al. The type 2 corticotrophin-releasing hormone receptor mediates orexin A-induced luteinising hormone suppression in ovariectomised rats. J Neuroendocrinol 2007; 19: 732-738.
• Johren O, Bruggemann N, Dendorfer A, Dominiak P. Gonadal steroids differentially regulate the messenger ribonucleic acid expression of pituitary orexin type 1 receptors and adrenal orexin type 2 receptors. Endocrinology 2003; 144: 1219-1225.
• Date Y, Mondal MS, Matsukura S, et al. Distribution of orexin/hypocretin in the rat median eminence and pituitary. Mol Brain Res 2000; 6: 1-6.
• Zhang S, Blache D, Vercoe PE, et al. Expression of orexin receptors in the brain and peripheral tissues of the male sheep. Regul Pept 2005; 124: 81-87.
• Suzuki H, Takemoto Y, Yamamoto T. Differential distribution of orexin-A-like and orexin receptor 1 (OX1R)-like immunoreactivities in the Xenopus pituitary. Tissue Cell 2007; 39: 423-430.
• Blanco M, Lopez M, Garcia-Caballero T, et al. Cellular localization of orexin receptors in human pituitary. J Clin Endocrinol Metab 2001; 86: 1616-1619.
• Kaminski T, Smolinska N, Nitkiewicz A, Przala J. Expression of orexin receptors 1 (OX1R) and 2 (OX2R) in the porcine pituitary during the oestrous cycle. Anim Reprod Sci 2010; 117: 111-118
• Seoane LM, Tovar SA, Perez D, et al. Orexin A suppresses in vivo GH secretion. Eur J Endocrinol 2004; 150: 731-736.
• Molik E, Zieba DA, Misztal T, Romanowicz K, Wszola M, Wierzchos E, Nowakowski M. The role of orexin A in the control of prolactin and growth hormone secretions in sheep - in vitro study. J Physiol Pharmacol 2008; 59(Suppl 9): 91-100.
• Samson WK, Taylor MM. Hypocretin/orexin suppresses corticotroph responsiveness in vitro. Am J Physiol Regul Integr Comp Physiol 2001; 281: R1140-R1145.
• Rak A, Gregoraszczuk EL. Modulatory effect of ghrelin in prepubertal porcine ovarian follicles. J Physiol Pharmacol 2008; 59(Suppl 4): 781-793.