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2004 | 53 | 2 |

Tytuł artykułu

Influence of DnaK and DnaJ chaperones on Escherichia coli membrane lipid composition

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The content of fatty acids extracted from the membranes of E. coli MC1061 harboring the wild-type dnaKdnaJ alleles and its AdnaJ and AdnaKdnaJ derivatives was compared. It was demonstrated that dodecanoic acid was missing in NPLs fraction extracted from both mutants grown at 42°C. Phospholipids extracted from mutant strains were deprived of hexadecanoic acid methyl ester and octadecanol, the latter being correlated with the presence of octadecanoic acid. The amount of LPS extracted from AdnaKdanJ mutant was significantly lower when compared with wild-type strain and AdnaJ mutant.

Wydawca

-

Rocznik

Tom

53

Numer

2

Opis fizyczny

p.121-123,ref.

Twórcy

autor
  • University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
autor
autor

Bibliografia

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  • Hartl F.U. 1996. Molecular chaperones in cellular protein folding. Nature 381: 571-580.
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  • Hitchock P.J. and T.M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154: 269-277.
  • Karpiński P., A.M. Grudniak and K.I. Wolska. 2002. Effect of mutations in dnaK and dnaJ genes on cysteine operon expression in Escherichia coli. Folia Microbiol. 47: 371-374.
  • Knox K., J. Cullen and E. Work. 1967. An extracellular lipopolysaccharide-phospholipid-protein complex produced by Escherichia coli grown under lysine-limited conditions. Biochem. J. 103: 192-201.
  • Liberek K., C. Georgopoulos and M. Żylicz. 1988. Role of the Escherichia coli DnaK and DnaJ heat shock proteins in initiation of bacteriophage lambda DNA replication. Proc. Natl. Acad. Sci. USA 85: 6632-6636.
  • Mayhew M. and F.U. Hartl. 1996. Molecular chaperone proteins, pp. 922-937. In: Neidhardt N.C., R. Curtiss III, J.L. Ingraham, E.C.C. Lin, K.B. Low, B. Magasanik, W.S. Resnikoff, M. Riley, M. Schaechter and H.E. Umbarger (eds). Escherichia coli and Salmonella, Cellular and Molecular Biology, 2nd ed., Vol. 1, Amer. Soc. Microbiol., Washington D.C.
  • McCarty J. and G.C. Walker. 1994. DnaK mutants defective in ATPase activity are defective in negative regulation of heat shock response: expression of mutant DnaK proteins results in filamentation. J. Bacteriol. 176: 764-780.
  • Ohki R., T. Kawamata, Y. Katoh, F. Hosoda and M. Ohki. 1992. Escherichia coli dnaJ mutation results in loss of stability of a positive regulator, CRP. J. Biol. Chem. 267: 13180-13184.
  • Paciorek J., K. Kardyś, B. Łobacz and K.I. Wolska. 1997. Escherichia coli defects caused by null mutations in dnaK and dnaJ genes. Acta Microbiol. Pol. 46: 7-17.
  • Sakakibara Y. 1988. The dnaK gene of Escherichia coli functions in initiation of chromosome replication. J. Bacteriol. 170: 972-979.
  • Tsuhido T., R.A. van Bogelen and F.C. Neidhardt. 1986. Heat shock response in Escherichia coli influences cell division. Proc. Natl. Acad. Sci. USA 83: 6959-6963.
  • Tsvetkdva N.M., I. Horvath, Z. Török, W.F. Wolkers, Z. Balogi, N. Shigapova, L.M. Crove, F. Tablin, E. Verling, J.H. Crove and L. Vigh. 2002. Small heat-shock proteins regulate membrane lipid polymorphism. Proc. Natl. Acad. Sci. USA 99: 13504-13509.
  • Wesphal O. and K. Jann. 1965. Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem. 5: 83-91.
  • Wild J., E. Altman, T. Yura and C.A. Gross. 1992. DnaK and DnaJ heat shock proteins participate in protein export in Escherichia coli. Genes Dev. 6: 1165-1172.
  • Wild J., P. Rossmeissl, W.A. Walter and C.A. Gross. 1996. Involvement of the DnaK-DnaJ-GrpE chaperone team in protein secretion in Escherichia coli. J. Bacteriol. 178: 3608-3613.
  • Wolska K.I., E. Bugajska, D. Jurkiewicz, M. Kuć and A. Jóźwik. 2000a. Antibiotic susceptibility of Escherichia coli dnaK and dnaJ mutants. Microb. Drug. Res. 6: 119-126.
  • Wolska K.I., B. Łobacz, D. Jurkiewicz, E. Bugajska, M. Kuć and A. Jóźwik. 2000b. Biosynthesis and secretion of several enzymes in Escherichia coli dnaK and dnaJ mutants. Microbios 101: 157-168.
  • Wolska K.I., J. Paciorek and K. Kardyś. 1999. Physiological consequences of mutations in Escherichia coli heat shock dnaK and dnaJ genes. Microbios 97: 55-67.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-752fbc05-bcea-451d-a244-23d9f29c93bd
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