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1999 | 45 | 2 |

Tytuł artykułu

Kryptosporydioza u osob zakazonych HIV

Treść / Zawartość

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Cryptosporidium parvum is a coccidian parasite that affects millions of people worldwide. Clinical outcome of human cryptosporidiosis differs between immunocompetent and immunodeficient individuals. C. parvum is responsible for causing protracted and life-threatening diarrhea, biliary, and pulmonary infections in immunocompromised persons, especially in patients with AIDS. Though no effective treatment has been found so far, early diagnosis may be useful in controlling the infection. Thirty-eight stool specimens obtained from 35 HIV-positive patients admitted to the Clinic of Infectious Diseases in Poznań, Poland, were examined for the detection of oocysts, coproantigen and DNA of Cryptosporidium using standard microscopic, immunologic and molecular diagnostic methods. The presence of Cryptosporidium was detected in 10 HIV-positive patients. Oocysts, coproantigen and DNA of this parasite were identified solely in one specimen while Cryptosporidium DNA was detected in 8 specimens. Cryptosporidium coproantigen was found only in one sample. Although, the PCR was the most useful technique in the detection of Cryptosporidium in HIV-positive patients it should be noted that PCR has many pitfalls and needs to be carefully controlled to avoid both false positive and false negative results.

Wydawca

-

Rocznik

Tom

45

Numer

2

Opis fizyczny

s.125-128,tab.,bibliogr.

Twórcy

autor
  • Akademia Medyczna,ul.Fredry 10, 61-701 Poznan
autor
autor
autor

Bibliografia

  • ANONIM. 1991. Basic laboratory methods in medical parasitology. WHO, Geneva, 18.
  • DUPONT C., BOUGNOUX M. E., TURNER L., ROUVEIX E., DORRA. 1996. Microbiological Findings about Pulmonary Cryptosporidiosis in Two AIDS Patients. J. Clin. Microbiol. 34: 227-229.
  • GARCIA L. S., BRUCKNER D. A 1997. In: Diagnostic Medical Parasitology. ASM Press, Washington, D. C., 3rd ed. 638-641.
  • MAJEWSKA A C., KASPRZAK W., WERNER A 1997. Prevalence of Cryptosporidium in mammals housed in Poznań Zoological Garden, Poland Acta Parasitologica 42: 195-198.
  • MEYNARD J.-L., MEYOHAS M.-C., BINET D., CHAUD C., FROTTIER J. 1996. Pulmonary Cryptosporidiosis in the Acquired Immunodeficiency Syndrome. Infection 24: 328-331.
  • MILAČEK P., VITOVEC J. 1985. Differential staining of cryptosporidia by aniline-carbol-methyl violet and tartrazine in smears from faeces and scrapings of intestinal mucosa. Folia Parasitologica 82: 50.
  • O'DONOGHUE P. J. 1995. Cryptosporidium and Cryptosporidiosis in Man and Animals. Int. J. Parasitol. 25: 139-195.
  • SULIMA P. 1998. DNA isolation from Cryptosporidium oocysts directly from stool samples for diagnostic goals using PCR. Wiad. Parozytol. 44: 301.
  • VAKIL N. B., SCHWARTZ S. M., BUGGY B. P., BRUMMIT C. F., KHERELLAH M., LETZER D. M., GILSON I. H., JONES P. G. 1996. Biliary cryptosporidiosis in HIV-infected people after the waterborne outbreak of cryptosporidiosis in Milwaukee. N. Engl. J. Med. 334: 19-23.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-74052ccc-ce40-4b26-9c5f-6c3cb6845f56
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