Carbon removal by activated sludge under fully aerobic conditions at different COD-N ratio

• Autorzy: Dobrzynska A , Wojnowska-Baryla I. , Bernat K.
• Języki publikacji: EN

Abstrakty

EN

The aim of the experiment was to analyze the particular processes (biomass synthesis, oxidation, denitrification and accumulation) during carbon removal from wastewater. The experiment was carried out in a sequencing batch reactor (SBR) in two series with different organic carbon compounds to nitrogen compounds ratio (COD/N). Organic carbon loading of activated sludge was 0.07 g COD/g·d. Organic carbon removal efficiency was higher at COD/N ratio 13 (98%), at COD/N ratio 3 (85%). Depending on COD/N ratio in waste, the processes of biomass synthesis, denitrification and intracellular accumulation changed. At low COD/N ratio biomass synthesis decreased; however, there was an increase in carbon used for denitrification and accumulation. I was observed that under fully aerobic conditions the activated sludge was able to store reserved materials expressed as poly(-β-hydroxybutyrate) PHB. When the lack of carbon source in waste was observed, the activated sludge used endogenous carbon resources as poly(-β-hydroxybutyrate) as electrons donor. Depending on activated sludge nitrogen loading, different times of endogenous denitrification were observed. In the first series the denitrification with endogenous carbon source was from the 8th hour in the second series during only 2 initial hours of the experiment. Biomass cellular respiration was on the level of 16% in both series.

Słowa kluczowe

EN

oxidation; waste water; denitrification; biomass synthesis; municipal waste water; biodegradable organic compound; activated sludge; organic carbon; carbon removal; poly[-beta-hydroxybutyrate]; accumulation; organic compound

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2004
• Tom: 13
• Numer: 1
• Opis fizyczny: p.33-40,fig.,ref.

Twórcy

autor

Dobrzynska A

• University of Warmia and Mazury in Olsztyn, Prawochenskiego 1, 10-957 Olsztyn, Poland

autor

Wojnowska-Baryla I.

autor

Bernat K.

Bibliografia

• 1. DIONISI D., MAJONE M., RAMADORI R., BECCARI M. The storage of acetate under anoxic conditions. Wat.Res., 35, 2661, 2001.
• 2. BARAK Y., VAN RIJN. Atypical polyphosphate accumulation by the denitrifying bacterium Paracoccus denitrificans. Appl. Environ. Microbiol., 66, (3), 1209, 2000.
• 3. NAKAMURA S., DOI Y. Microbial synthesis and characterization of poly (3-hydroksybutyrate-co-4-hydroxybutyrate). Macromolecules., 25, 4237, 1992.
• 4. JAMES B.W., MAUCHLINE W.S., DENNIS P.J., KEEWIL C.W., WAIT R. Poly-3 hydroxybutyrate in Legionella pneumophila, an energy source for survival in low nutrient environments. Appl. Environ. Microbiol., 65, (2), 822, 1999.
• 5. SUN J., PENG X., IMPE VAN J., VANDERLEYDEN J. The nitrB and nitrC genes are involved in the regulation of poly-3-hydroxybutyrate biosynthesis by amonia in Azospirillum brasilense Sp7. Appl. Environ.Microbiol., 66, 113, 2000.
• 6. ANSEJO J.A., SCHMIDT A.S., ANDREWS B.A. Effects of single nutrient limitation on poly-β-hydroksybutyrate molecular weight disrtibution in Alcaligenes eutrophus. Biotechnol. Bioeng., 46, 497, 1995.
• 7. KANSIZ M., BILLMAN-JACOBE H., MCNAUGHTON D. Quantitative determination of the biodegradable polymer poly(β-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics. Appl. Environ. Microbiol., 66, (8), 3415, 2000.
• 8. CHOI J., LEE S.Y. Efficient and economical recovery of poly(β-hydroxybutyrate) from recombinant Escherichia coli by simple digestion with chemicals. Biotechnol. Bioeng., 62, (5), 546, 1999.
• 9. CARTA F., BEUN J.J., VAN LOOSDRECHT M.C.M. Simultaneous storage and degradation of PHB and glycogen in activated sludge cultures. Appl. Environ. Microbiol., 62, (5), 856, 2000.
• 10. BEUN J.J., VERHOEF E.V., VAN LOOSDRECHT M.C.M. Stechiometry and kinetics of poly – β- hydroxibutirate metabolism under denitryfying conditions in activated sludge cultures. Biotechnol. Bioeng., 68, 496, 2000.
• 11. BARNARD J.L., OLESZKIEWICZ J.A. Wastewater characteristic and the examples of its impact on the volume of activated sludge chamber. Designing philosophy and wastewater treatment plants operation. Training seminary materials. Lem Project s. c., Kraków, Poland, 239 248, 2000.
• 12. JENKINS D., RICHARD M.G., DAIGGER G. Manual on the causes and control of activated sludge bulking and foaming. Wat. Res. Commission Pretoria, 30, 1986.
• 13. NOVAK T. J. The effect of the ammonium ion on activatedsludge settling properties. Wat. Environ. Res., 73, 409, 2001.
• 14. RENNER G., HAAGE G., BRAUNEGG G. Production of short-side-chain polyhydroxyalkanoates by various bacteria from the rRNA superfamily III. Appl. Microbiol. Biotechnol., 46, 268, 1996.
• 15. MAJONE M., DIRCKS K., BEUN J. Aerobic storage under dynamic conditions in the activated sludge processes. The state of the art. Water Sci. Technol., 39, 61, 1999.
• 16. YAMANE T., FUKUNAGA M., LEE Y.W. Increased PHB productivity by high-cell-density fed-batch culture of Alcaligenes latus, a growth-associated PHB producer. Biotechnol. Bioeng., 50, 197, 1996.
• 17. MAJONE M., MASSANISSO P., RAMADORI R. Comparision of carbon storage under aerobic and anoxic conditions. Wat. Sci. Technol., 38, 77, 1998.