The impact of UV mediated hydrogen peroxide on culturable bacteria in the surface microlayer of eutrophic lake

• Autorzy: Walczak M , Swiontek Brzezinska M.
• Języki publikacji: EN

Abstrakty

EN

Hydrogen peroxide (H₂O₂) formation in surface waters is initiated by the absorption of sunlight by dissolved organic matter (DOM). The fraction of the DOM pool that interacts with sunlight, referred to as chromophoric dissolved organic matter, impacts the optical properties of surface waters. Second source of H₂O₂ is wet and dry deposition of photogenerated substance in the atmosphere and biological production. The study examined the concentration of hydrogen peroxide in water from the surface microlayer (SM) (<100 m) and subsurface water (SSW) (25 cm) in the typical eutrophic (TOC 5–15 mg dm⁻³; chlorophyll 5–26 g dm⁻³, water transparency 0.6–1.0 m) lake as well as the impact of this compound on occurrence and survivorship of catalase-positive and catalase-negative bacteria isolated and cultured on the TSA medium (Difco). The experimental H₂O₂ concentrations ranged between 500–5000 nM. The concentration of H₂O₂ in analyzed water samples clearly increased in day-time hours and was different in May, July and October. The highest natural concentration of H₂O₂ (700 nM) was observed in SM water in summer in afternoon hours. During that period, 100% of bacterial populations found in SM water produced catalase. The experiments confirmed that environmental concentrations of H₂O₂ caused no considerable decrease in survivorship of culturable bacteria, while concentrations exceeding 1000 nM were lethal for the majority of catalasenegative bacteria, but not for catalase-positive bacteria.

Słowa kluczowe

EN

surface microlayer; hydrogen peroxide; bacteria; catalase; surface water; absorption; optical property; dissolved organic matter; eutrophic lake

• Wydawca: -
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2009
• Tom: 57
• Numer: 3
• Opis fizyczny: p.547-554,fig.,ref.

Twórcy

autor

Walczak M

• Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland

autor

Swiontek Brzezinska M.

Bibliografia

• Bartosz G. 2004 – Druga twarz tlenu [Second face of oxygen] – PWN, Warszawa. (in Polish).
• Cantoni O.P., Sestili A., Guidarelli P., Giacomoni U., Cattabeni F. 1992 – Effects of L-histidine on hydrogen peroxide – induced DNA damage and cytotoxicity in cultured mammalian cells – Mol. Pharmacol. 41: 969 – 974.
• Cooper W.J., Zika R.G., Petasne R.G., Plane J.M. 1988 – Photochemical formation of H₂O₂ in natural waters exposed to sunlight – Environ. Sci. Technol. 22: 1156–1160.
• Cooper W.J., Lean D.R.S. 1989 – Hydrogen peroxide concetration in a northern lake: photochemical formation and diel variability – Environ. Sci. Technol. 23: 1425–1428.
• Corin N., Backlund P., Wiklund T. 1998 – Bacterial growth in humic waters exposed to UV radiation and simulated sunlight – Chemosphere, 36: 1947–1958.
• Donderski W., Walczak M., Mudryk Z., Kobyliński M. 1999 – Neustonic bacteria number, biomass and taxonomy – Pol. J. Environ. Stud. 8: 137–141
• Elkins J.G., Hassett D.J., Stewart P.S., Schweizer H.P., McDermott T.R. 1999 – Protective role of catalase in Pseudomonas aeruginosa biofilm resistance to hydrogen peroxide – App. Environ. Microbiol. 65: 4594–4600.
• Garrett W.P. 1965 – Collection of slick-forming materials from the sea surface – Limnol. Oceanogr. 10: 602 – 605.
• Herut B., Shonam-Frider E., Kress N., Fiedler U., Angel D.L. 1998 – Hydrogen peroxide production rates in clean and polluted coastal marine waters of the Mediterranean, Red and Baltic seas – Mar. Poll. Bull. 36: 994–1003.
• Hillbricht-Ilkowska A., Kostrzewska-Szlakowska I. 2004 – Surface microlayer in lakes of different trophic status: nutrients concentration and accumulation – Pol. J. Ecol. 52: 461–478.
• Kostrzewska-Szlakowska I. 2005 – Surface microlayer in lakes of different trophic status: dissolved organic matter and microbial community – Pol. J. Ecol. 53: 341–351.
• Maki J.S. 1993 – The air-water interface as an extreme environment (In: Aquatic Microbiology: An ecological approach, Ed. T.F. Edgcumbe) – Blackwell Scientific Publications, Boston, pp. 409–440.
• Maki J.S., Remsen C.C. 1989 – Examination of a freshwater surface microlayer for diel changes in the bacterioneuston – Hydrobiologia, 182: 25 – 34.
• O’Sullivan D.W., Neale P.J., Coffin R.B., Boyd T.J., Osburn C.L. 2005 – Photochemical production of hydrogen peroxide and methylhydroperoxide in coastal waters – Mar. Chem. 97: 14–33.
• Petasne R.G., Zika R.G. 1997 – Hydrogen peroxide lifetimes in south Florida coastal and offshore waters – Mar. Chem. 56: 215–225.
• Price D., Mantoura R.F., Worsfold P.J. 1998 – Shipboard determination of hydrogen peroxide in the western Mediterranean sea using flow injection with chemiluminescence detection – Anal. Chem. Acta, 371: 205–215.
• Scully N.M., Cooper W.J., Tranvik L.J. 2003 – Photochemical effects on microbial activity in natural waters: the interaction of reactive oxygen species and dissolved organic matter – FEMS Microbiol. Ecol. 46: 353–357.
• Scully N.M., Lean D.R.S., McQueen D.J., Cooper W.J. 1996 – Hydrogen peroxide formation: The interaction of ultraviolet radiation and dissolved organic carbon in lake waters along a 43 75N gradient – Limnol. Oceanogr. 41: 540–548.
• Xenopoulos M.A., Bird F.B. 1997 – Effect of acute exposure to hydrogen peroxide on the production of phytoplankton and bacterioplankton in mesohumic lake – Photochem. Photobiol. 66: 471–478.