Ras oncogene product p21 expression in adenocarcinoma of human endometrium

• Autorzy: Miturski R. , Semczuk A. , Jakowicki J.A.
• Języki publikacji: EN

Abstrakty

EN

ras-encoded p21 protein expression was investigated immunohistochemically in 10 specimens of human endometrial adenocarcinoma. The control group consisted of 5 proliferative endometrium slides. The analysis was performed by using the biotin-streptavidin-peroxidase AEC detection system. An immunohistochemical histologic H- score according to Nyholm et al. [1] was calculated in a semiquantitative fashion incorporating intensity and distribution of staining. Adenocarcinoma glandular cells stained positively in 6 out of 10 specimens (n=6; H-score 0.71; range 0.3-1.15), whereas adenocarcinoma stromal cells exhibited a reddish-brown color in all specimens analyzed (n=10; H-score 0.83; range 0.3-1.5). Only one out of 5 proliferative endometrium slides stained positively for ras p21 (H-score 0.3). Our observations suggest that in human endometrial adenocarcinomas not only glandular but also stromal cells reacted strongly with monoclonal antibody NCC-RAS-001 raised against ras p21 protein. Enhanced ras p21 expression adenocarcinoma stromal cells in comparison with adenocarcinoma glandular cells may suggest that stromal cells are involved strongly in the neoplasm formation.

Słowa kluczowe

EN

oncogenesis; human endometrium; man; adenocarcinoma; oncogene product

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1996
• Tom: 01
• Numer: 2
• Opis fizyczny: p.145-149,fig.

Twórcy

autor

Miturski R.

• University School of Medicine, 8 Jaczewski str., 20-095 Lublin, Poland

autor

Semczuk A.

autor

Jakowicki J.A.

Bibliografia

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