In vitro cholesterol uptake by Lactobacillus acidophilus isolates

• Autorzy: Ziarno M.

Warianty tytułu

PL

Usuwanie cholesterolu w warunkach in vitro przez izolaty Lactobacillus acidophilus

• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate cholesterol uptake by Lb. acidophilus isolates in artificial gastric and duodenal fluids. Studied Lb. acidophilus isolates showed various abilities to uptake cholesterol from MRS broth and artificial GIT fluids. From artificial duodenal fluid Lb. acidophilus isolates removed morę cholesterol (from 0.6% to 2.9%) than from gastric fluid (from 3.8% to 13.5%). Even if bacteria had no possibility to grow in artificial GIT fluids, the cholesterol uptake by bacteria cells took place. Lb. acidophilus isolates survived worse in simulated gastric or duodenal fluids than in MRS broth. The initial number of bacteria ranged from 6.2 log CFU/mL to 7.1 log CFU/mL, and viability in artificial gastric fluid was 60.6-81.9% of log of the initial number of bacteria. Viability in artificial duodenal fluid was 35.1-87.3%.

PL

Celem badań było określenie zdolności izolatów Lb. acidophilus do usuwania cholesterolu w warunkach modelowego soku żołądkowego i modelowego soku trzustkowego. Badane izolaty Lb. acidophilus wykazały się różną zdolnością do usuwania cholesterolu z bulionu MRS i modelowych soków trawiennych. Z modelowego soku trzustkowego izolaty Lb. acidophilus usunęły więcej cholesterolu (od 0,6% do 2,9%) niż z modelowego soku żołądkowego (od 3,8% do 13,5%). Ubytek cholesterolu był obserwowany, nawet jeśli komórki bakterii nie miały możliwości rozwoju w modelowych sokach trawiennych. Izolaty Lb. acidophilus przeżywały gorzej w modelowym soku żołądkowym lub modelowym soku trzustkowym niż w bulionie MRS. Początkowa liczba bakterii była w zakresie 6,2-7,1 log jtk/cm3, a przeżywalność w modelowym soku żołądkowym wyniosła 60,6-81,9% logarytmu liczby początkowej bakterii. Przeżywalność w modelowym soku trzustkowym osiągnęła 35,1-87,3% logarytmu liczby początkowej bakterii.

Słowa kluczowe

EN

cholesterol; Lactobacillus acidophilus; cholesterol uptake; simulated intestinal juice; simulated duodenal juice; in vitro; bacterial cell; gastric fluid

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Technologia Alimentaria
• Rocznik: 2008
• Tom: 07
• Numer: 3
• Opis fizyczny: p.65-74,ref.

Twórcy

autor

Ziarno M.

• Warsaw University of Life Sciences, Nowoursynowska 159 C, 02-787 Warsaw, Poland

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