Bile acid transport in hypercholesterolemic resistant rabbits

• Autorzy: Izzat N.N. , Overturf M. , Weisbrodt N.W. , Loose D.S.
• Języki publikacji: EN

Abstrakty

EN

We examined bile acid transport and expression of the apical sodium-dependent bile acid transporter (ASBT) in ileal preparations to determine if alterations in bile acid excretion contributed to a hypercholesterolemia-resistant phenotype in rabbits (CRT/mlo). Taurocholate transport was not different between normal (NR) and CRT/mlo rabbits fed regular diet. However, feeding cholesterol-enriched diet reduced taurocholate transport significantly in CRT/mlo rabbits (0.53±0.06 pmol/µg protein) compared to regular diet (0.95±0.14 pmol/µg protein), but had no effect in NR rabbits. Cholesterol-enriched diet increased ASBT mRNA in CRT/mlo (2.6 ± 0.7 to 5.4 ± 0.1); no significant changes occurred in NR. Some CRT/mlo rabbits carry a polymorphism in ASBT at amino acid 333 (P333L). In transfected HEK293 cells, TC transport of P333L allele was significantly lower (0.08 ± 0.01 vs 0.13 ± 0.01 pmol/µg protein/15 sec, P< 0.05). This allele was not found in NR rabbits. The data suggest that the phenotype of the CRT/mlo rabbit is due to changes in bile acid transport as well as bile acid metabolism.

Słowa kluczowe

EN

bile acid transport; hypercholesterolemia; hypocholesterolemia; rabbit; plasma cholesterol; cholesterol; steroid hormone; homeostasis

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 3
• Opis fizyczny: p.79-84,fig.,ref.

Twórcy

autor

Izzat N.N.

• University of Texas Health Science, Houston, TX 77030, USA

autor

Overturf M.

autor

Weisbrodt N.W.

autor

Loose D.S.