Nucleosomes and regulation of gene expression. Structure of the HIV-1 5'LTR

• Autorzy: Widlak P. , Garrard W.T.
• Języki publikacji: EN

Abstrakty

EN

Packaging of DNA into chromatin adds complexity to the problem of regulation of gene expression. Nucleosomes affect the accessibility of transcription factors to occupy their binding sites in chromatin of eukaryotic cells. The disruption of nucleosome structure within the enhancer/promoter region of the integrated HIV-1 proviral genome is an instructive example of a chromatin remodeling process during transcriptional activation. To investigate the mechanism responsible for generating nuclease hypersensitive sites that exist in vivo in the promoter/enhancer region of the 5'LTR (long terminal repeat) of integrated HIV-1 we have utilized an in vitro chromatin assembly system with Xenopus oocyte extracts. Chromatin assembly in the presence of Sp1 and NFκB transcription factors induces DNase I hypersensitive sites on either side of their binding sites and positions the adjacent nucleosomes. This structure can also be formed in a factor-induced, ATP-dependent chromatin remodeling process and closely resembles the in vivo chromatin structure. The DNase I hypersensitive sites that form within the HIV LTR are probably histone-free and remain after removal of transcription factors.

Słowa kluczowe

EN

chromatin structure; HIV-1; eukaryotic cell; long terminal repeat; gene expression; HIV-1 structure; DNA; micrococcal nuclease; nucleosome remodelling factor

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 1
• Opis fizyczny: p.209-219,fig.

Twórcy

autor

Widlak P.

• Institute of Oncology, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland

autor

Garrard W.T.

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