The modulation of transferrin receptors level on mouse macrophages and fibroblasts by Toxoplasma gondii

• Autorzy: Dziadek B , Dytnerska-Dzitko K. , Dlugonska H.
• Języki publikacji: EN

Abstrakty

EN

Macrophage-mediated early nonspecific immunological response is an important part of the immunity against intracellular parasite Toxoplasma gondii. The immunological functions of macrophages are closely connected with iron metabolism and acquiring of iron mainly from transferrin by the receptor-mediated endocytosis. The level of specific transferrin receptors can be modulated by different soluble exogenous and endogenous factors and also by microbial pathogens. The goal of our study was to determine the influence of T. gondii infection and toxoplasma lysate antigen (TLA) on the expression level of transferrin receptors (TfRs) on mouse macrophages and fibroblasts which can serve as host cells for the parasite replication. The level of TfRs was measured using CELISA assay. Strong down-regulation of the receptors level, started about 18 hours after infection of macrophages with a high number of freshly harvested tachyzoites T. gondii. Stimulation of the mouse cells with TLA antigen did not cause any changes in TfRs expression. In our studies we did not observe any differences in the TfRs level on mouse fibroblasts even after incubation with high concentrations of TLA antigen or inoculation with a high number of tachyzoites.

Słowa kluczowe

EN

mouse; mice; macrophage; Toxoplasma gondii; fibroblast; transferrin receptor; intracellular parasite

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: Suppl.
• Opis fizyczny: p.75-80,fig.,ref.

Twórcy

autor

Dziadek B

• University of Lodz, Banacha 12-16 Str., 90-237 Lodz, Poland

autor

Dytnerska-Dzitko K.

autor

Dlugonska H.

Bibliografia

• Aisen P., M. Wessling-Resnick and E.A. Leibold. 1999. Iron metabolism. Curr. Opin. Chem. Biol. 3: 200-206.
• Aisen P., C. Enns and M. Wessling-Resnick. 2001. Chemistry and biology of eukaryotic iron metabolism. Int. J. Biochem. Cell Biol. 33: 940-959.
• Boothroyd J.C., A. Hehl, L.J. Konoll and I.D. Manger. 1998. The surface of Toxoplasma: more and less. Int. J. Parasitol. 28: 3-9.
• Britigan B.E., R.W. Vorhies and M.E. Wilson. 1994. Acquisition of iron from transferrin and lactoferrin by the protozoan Leishmania chagasi. Infect. Immun. 62: 3262-3269.
• Byrd T.F. and M.A. Horwitz. 2000. Aberrantly low transferrin receptor expression on human monocytes is associated with nonpermissiveness for Legionella pneumophila growth. J. Infect. Dis. 181: 1394-1400.
• Denkers E.Y. and R.T. Gazzinelli. 1998. Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection. Clin. Microbiol. Rev. 11: 569-588.
• Denkers E.Y, B.A. Butcher, L. Del Rio and S. Bennouna. 2004. Neutrophils, dendritic cells and Toxoplasma. Int. J. Parasitol. 34: 411-421.
• Dimier I.H. and D.T. Bout. 1993. Rat intestinal epithelial cell line IEC-6 is activated by recombinant interferon-y to inhibit replication of the coccidian Toxoplasma gondii. Eur. J. Immunol. 23: 981-983.
• Dimier I.H. and D.T. Bout. 1998. Interferon-y-activated primary enetrocytes inhibit Toxoplasma gondii replication: a role for intracellular iron. Immunology 94: 488-495.
• Furtado G.C., M. Słowik, H.K. Kleinman and K.A. Joiner. 1992. Laminin enhances binding of Toxoplasma gondii tachyzoites to J774 murine macrophage cells. Infect. Immun. 60: 2337-2342.
• Gail M., U. Gross and W. Bohne. 2001. Transcriptional profile of Toxoplasma gondii-infected human fibroblasts as revealed by gene-array hybridization. Mol. Genet. Genomics 265: 905-912.
• Galbraith R.M. and G.M. Galbraith. 1983. Expression of transferrin receptors on mitogen-stimulated human peripheral blood lymphocytes: relation to cellular activation and related metabolic events. Immunobiology 133: 703-710.
• Gazzinelli R.T., D. Amichay, T. Scharton-Kersten, E. Grunvald, J.M. Farber and A. Sher. 1996. Role of macrophage-derived cytokines in the induction and regulation of cell-mediated immunity to Toxoplasma gondii. Curr. Top. Microbiol. Immunol. 219: 127-140.
• Grimwood J. and J.E. Smith. 1996. Toxoplasma gondii: the role of parasite surface and secreted proteins in host cell invasion. Int. J. Parasitol. 26: 169-173.
• Hauser W.E. and V. Tsai. 1986. Acute toxoplasma infection of mice induces spleen NK cells that are cytotoxic for T. gondii in vitro. J. Immunol. 136: 313-319.
• Jacquet A., L. Coulon, J. De Neve, V. Daminet, M. Haumont, L. Garcia, A. Bollen, M. Jurado and R. Biemans. 2001. The surface antigen SAG3 mediates the attachment of Toxoplasma gondii to cell-surface proteoglycans. Mol. Biochem. Parasitol. 116: 35-44.
• Lima M.F. and F. Villalla. 1990. Trypanosoma cruzi receptors for human transferrin and their role. Mol. Biochem. Parasitol. 38: 245-252.
• Manger I.D., A.B. Hehl and J.C. Boothroyd. 1998. The surface of Toxoplasma tachyzoites is dominated by a family of glycosylphosphatidylinositol-anchored antigens related to SAG1. Infect. Immun. 66: 2237-2244.
• Mulero V. and J.H. Brock. 1999. Regulation of iron metabolism in murine J774 macrophages: role of nitric oxide-dependent and -independent pathways following activation with gamma interferon and lipopolisaccharide. Blood 94: 2383-2389.
• Ortega-Barria E. and J.C. Boothroyd. 1999. A Toxoplasma lectin-like activity specific for sulfated polysaccharides is involved in host cell infection. J. Biol. Chem. 274: 1267-1276.
• Ponka P. and C.N. Lok. 1999. The transferrin receptor: role in health and disease. Int. J. Biochem. Cell. Biol. 31: 1111-1137.
• Qian Z.M. and P.L. Tang. 1995. Mechanisms of iron uptake by mammalian cells. Biochim. Biophys. Acta 1269: 205-214.
• Sher A., I.O. Oswald, S. Hieny and R.T. Gazzinelli. 1993. Toxoplasma gondii induces a T-independent IFN-gamma response in natural killer cells that requires both adherent accessory cells and tumor necrosis factor-alpha. J. Immunol. 150: 3982-3989.
• Steverding D., Y.D. Stierhof, H. Fuchs, R. Tauber and P. Overath. 1995. Transferrin-binding protein complex is the receptor for transferrin uptake in Trypanosoma brucei. J. Cell Biol. 131: 1173-1182.
• Weinberg E.D. 2000. Modulation of intramacrophage iron metabolism during microbial cell invasion. Microbes. Infect. 2: 85-89.
• Wilson M.E. and B.E. Britigan. 1998. Iron acquisition by parasitic protozoa. Parasitol. Today 14: 348-353.
• Zhong W., W.P. Lafuse and B.S. Zwilling. 2001. Infection with Mycobacterium avium differentially regulates the expression of iron transport protein mRNA in murine peritoneal macrophages. Infect. Immun. 69: 6618-6624.