Effect of coal dusts containing heavy metals on structure, cellular enzymatic markers, and cytochrome P-450-dependent monooxygenase system in rat kidney

• Autorzy: Trzeciak H. I. , Kaminski M. , Plewka A. , Malecki A. , Pudelko A.
• Języki publikacji: EN

Abstrakty

EN

We evaluated morphological changes, the activities of succinate dehydrogenase, lactate dehydrogenase, glucose-6-phosphatase, Mg2+ -dependent adenosine triphosphatase, and acid phosphatase, and the activity of the cytochrome P-450-dependent monooxygenase system in the kidneys of rats exposed to coal dusts containing either very low or high amounts of heavy metals. We showed that the coal dust with a very low content of heavy metals (VLCHM) did not produce any structural lesions, but increased significantly the activities of the enzymes. These changes probably reflected kidney adaptation to the unfavorable conditions caused by the metals. The interactions among them reduced the eventual nephrotoxic effect of their action. The coal dust with a high content of heavy metals (HCHM) damaged proximal convoluted tubules, straight tubules, and, to a lesser degree, distal convoluted tubules, especially those of long-looped nephrones. The levels and the activities of the assayed cytochromes decreased significantly in the kidneys of rats exposed to HCHM coal dust.

Słowa kluczowe

EN

enzymatic marker; structure; coal dust; kidney; cytochrome P450; rat; heavy metal

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 1996
• Tom: 05
• Numer: 2
• Opis fizyczny: p.49-54,fig.,ref.

Twórcy

autor

Trzeciak H. I.

• Silesian Academy of Medicine, 18 Medykow Street, 40-752 Katowice-Ligota, Poland

autor

Kaminski M.

autor

Plewka A.

autor

Malecki A.

autor

Pudelko A.

Bibliografia

• BRZOZOWSKA A. Harmful elements versus iron, zinc and copper: interactions in the animal and human organism. Part III. Cadmium (in Polish). Roczn. PZH 3, 269-276, 1991
• COOGANT T.P., LATTA D.M., SNOW E.T ., COSTA M. Toxicity and carcinogenicity of nickel compounds CRC. Crit. Rev. Toxicol. 19, 341-384, 1989.CHAN M.H., SATOH M., ZALUPS R.K., CHERIAN M.G. Exogenous metallothionein and renal toxicity of cadmium and mercury in rats. Tocicology 76, 15-26, 1992.
• CZEKAJ P., KAMIIQSKI M. Linked monooxygenase systems in kidney (in Polish). Post. Hig. Med. Dośw., 42, 641-664, 1988.
• DALLNER G. Isolation of microsomal subfraction by use of density gradients. Methods Enzymol., 52, 71-83, 1978.
• DEWISON M.G., KANARIS-SOTIRION A. The determination of trace elements in whole coal by rhodinum tube x-ray fluores- cence spectrometry, 21 rock based synthetic coal calibration. Int. ] . Coal Geol., 6, 627-641, 1986.
• ESTABROOK R.W., WERRINGLOER J. The measurement of difference spectra: Aplication to the cytochromes of microsomes. Methods Enzymol., 52, 212-220, 1978.
• EVERING W.E., HAYWOOD S., ELMES E., JASANI B., TRAFFORD J. Histochemical and immunocytochemical evaluation of copper and metallothionein in the liver and kidney of copper-loaded rats. J. Pathol., 160, 305-312, 1990.
• FLOWER B.A. Mechanisms of kidney cell injury from metals. Environ. Health Perspect, 100, 57-63, 1992.GOMORI G. Microscopic Histochemistry Principles and Practice, Chicago, University Chicago Press, pp. 131-133. 1953.
• GOYER R. Lead toxicity: Current concerns. Environ. Health Perspect., 100, 177-187, 1993.
• HAŁATEK T., CHMIELNICKA J. Evaluation of nephrotoxicity of cadmium in laboratory animals and humans (in Polish). Post. Hig. Dośw. 5, 375-391, 1993.
• HAVRANCOVA J., SKODA V., HOLUSA R., KUBACKOVA J. Obsach lyzozymu a medi v serech potkanu po podani prachu. Bratisl. Lek. Listy 91, 111-114, 1990.
• HODGEST T.K., LEONARD R.T. Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods Enzymol. 32, 392-406, 1974.
• KAMINSKI M. Localization of the mixed function oxidases in organism (in Polish). Folia Med. Cracov. 31, 97-120, 1990.
• LOWRY O.H., ROSENBROGH N.J., FARR A.L., RANDALL J. Protein measurment in the Follin phenol reagent. J. Biol. Chem. 193, 265-275, 1951.
• MAŁECKI A. Effect of heavy metals in coal dust on animals. Doctoral Thesis (in Polish). Silesian Academy of Medicine, Katowice, 1993.
• PEARSE A.G.E. Histochemistiy. Teoretical and Applied, J. A. Churchill, London. pp 132-140, 1960.
• PINTA M., RIANDEY C. Determination des traces dans les etles roches par spectrophotometrie diabsorption atomique. Meth. Phys. Anal, 5, 76-92, 1969.
• PISKORSKA-KALISZ Z. Methods of Measuring Physicochemical Properties of Mineral Dust (in Polish), Post-Graduate Training Center in Chorzów-Batory (ed.), Chorzów, 1990.
• RAO N.V. Elemental analysis of coal ash using energy dispersive x-ray fluorescency. X-ray Spectrometry 16, 147-149, 1987.
• SAYGI S., DENIZ G., KUTSAL O., VURAL N. Chronic effects of cadmium on kidney, liver, testis and ferility of male rats. Biol. Trace Elem. Res. 31, 209-214, 1991.
• SPARROW S., MAGOS L., SNOWDEN R. The effect of sodium chromate pretreatment on mercuric chloride-induced nephrotoxicity. Arch. Toxicol., 61, 440-443, 1988.
• SRIVASTAVA V.K., CHAUHAN S.S., SRIVASTAVA P.K., SKUHLA R.R., KUMAR V., MISRA U.K. Placental transfer of metals of coal fly ash into various fetal organs of rat. Arch. Toxicol., 64, 153-156, 1990.
• STEIBERT E., KRÓL B., SOWA B., GRALEWSKA K., KAMINSKI M., KAMINSKA o., KUSZ E. Cadmium-indeced changes in the histoenzymatic activity in liver, kidney and duodenum of pregnant rats. Toxicol. Letters, 20, 127-132, 1984.
• STRĘK F., HABA A., ROCHOWIECKI A., GÓRA W. The toxicological Chamber for investigations of dusts and toxic gases (in Polish). Med. Pracy, 21, 45-52, 1970.
• SZYMANSKA J.A., BEM E.M., PIOTROWSKI J.K., BRZEŻ- NICKI S., BARAN T. Renal binding of cadmium in the rat following intragastric exposure. Tóxicology 55, 339-348, 1989.
• TRZECIAK H.I., KAMINSKI M., PLEWKA A., MAŁECKI A., GARBYEL B. Effects of coal dusts with different concentrations of metals on structure, hepatocellular enzymatic markers, and cytochrome P-450-dependent monooxygenase system in rat liver. Pol. J. Environ. Stud. (in press).
• WACHSTEIN M., MEISEL E. Histochemistry of hepatic phosphatases at physiol pH with special reference to the demonstration of bile canaliculi. Am. J. Clin. Pathol., 27, 12-23, 1957.