The detection and purification of a protein that recognizes a line 1 DNA repetitive sequence

• Autorzy: Lukyanov D. , Rogolinski J. , Podgornaya O. , Rzeszowska-Wolny J.
• Języki publikacji: EN

Abstrakty

EN

A protein which binds specifically to MspI8 (a 454 bp long repeated sequence highly homologous to the 5’ untranslated region (5'UTR) of the LINE 1 sequence) was found and identified in nuclear extracts of rat liver cells. This protein was detected using the electrophoretic mobility shift assay (EMSA) and was purified by Q-Sepharose and DNA affinity chromatography. Its molecular mass was estimated by SDS electrophoresis as 29 kDa. The possibility that this protein (p29) is the rat analogue of human L1PBP-A, specific for the human LINE sequence LPE1, is discussed.

Słowa kluczowe

EN

detection; purification; protein; repetitive DNA sequence; binding protein; rat; liver cell

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2000
• Tom: 05
• Numer: 4
• Opis fizyczny: p.511-521,fig.,ref.

Twórcy

autor

Lukyanov D.

• Russian Academy of Sciences, Tikhoretsky pr.4, 194064 St.Petersburg, Russia

autor

Rogolinski J.

autor

Podgornaya O.

autor

Rzeszowska-Wolny J.

Bibliografia

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