Effect of mechanical pressure on c-fos and on the mitotic activity of epidermal cells

• Autorzy: Tsanev R. , Yaneva J. , Vaptzarova K. , Markov D.
• Języki publikacji: EN

Abstrakty

EN

A dosed mechanical pressure of 12.5 kg/cm2 applied for 1 min on depilated mouse skin did not cause cellular death or visible alterations of the cellular infrastructures. However, it had a strong effect on the mitotic cycle of the epidermal cells—stimulating the cells to enter the mitotic cycle and temporarily blocking the Gl -> S transition. This effect was strictly limited to the pressed area of the skin. The proto-oncogene c-fos was induced within the first 2 min following application of the pressure. The level of c-fos mRNA showed two peaks during the next 24 h. The first slight peak was preceded by a rapid increase in the cAMP level in the pressed skin, the second — by a fall in the cAMP concentration. A model is suggested to explain the observed effects by reversible functional damage of the cellular membrane affecting the enzymes maintaining the steady state level of cAMP.

Słowa kluczowe

EN

epidermal cell; skin; cell cycle; cell membrane; mouse; photo-oncogene; mice; mitotic activity

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 4
• Opis fizyczny: p.593-601,fig.

Twórcy

autor

Tsanev R.

• Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

autor

Yaneva J.

autor

Vaptzarova K.

autor

Markov D.

Bibliografia

• 1. Angel, P. & Karin. M. (1991) The role of Jun, Fos and the AP-1 complex in cell proliferation and transformation. Biochim. Biophys. Acta 1072, 129-157.
• 2. Van Beveren, C., van Straaten, F., Curran, T., Muller, R. & Verma, I.M. (1983) Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini. Cell 32, 1241- -1255.
• 3. Basset-Seguin, N., Escot, C., Blanchard, J.M., Kerai, C., Verrier, B., Mion, H. & Guilhou, J.J. (1990) High levels of c-fos proto-oncogene expression in normal human adult skin. J. Invest. Dermatol. 94,418-422.
• 4. Basset-Seguin, N., Escot, C., Moles, J.P., Blanchard, J.M., Kerai, C & Guilhou. J.J. (1991) C-fos and c-jun proto-oncogene expression is decreased in psoriasis: An in situ quantitative analysis. /. Invest. Dermatol. 97,672-678.
• 5. Briata, P., IX Anna, F., Franzi, A.T. & Gherzi, R. (1993) AP-1 activity during normal human keratinocyte differentiation: Evidence for a cytosolic modulator of API / DNA binding. Cxp. Cell Res. 204,136-146.
• 6. Elder, J.T., Tavakkol, A., Klein, S.B., Zeigler, M.E., Wicha, M. & Voorhees, J.J. (1990) Protooncogene expression in normal and psoriatic skin. /. Invest. Dermatol. 94,19-25.
• 7. Tsanev, R. (1962) Injury-induced changes in nucleic acid content of the epidermis. Bull. Biochem. Res. Lab., Bulg. Acad. Sci. 1,7-13.
• 8. Tsanev, R. & Markov, G. (1960) Substances interfering with spectrophotometric estimation of nucleic acids and their elimination by the two-wavelength method. Biockim. Biophys. Acta 42,442-452.
• 9. Pepin, R.A., Lucas, D., Lang, R.B., Lee, N., Uao, M.-J. & Testa, D. (1990) Detection of picogram amounts of nucleic acid by dot blot hybri­dization. Biotechniques 8,628^632.
• 10. Kikuchi, K. & Makishima, F. (1990) Tissue-specific expression of m-RNA in mouse lymphocytes detected by v-fos but not by human c-fos DNA probes. Cell Struct. Fund. 15, 323-328.
• 11. Gilman, A.G. (1987) G proteins: transducers of receptor-generated signals. Annu. Rev. Biochem. 56,615-649.
• 12. Gornall, A., Bard will, J. & David, N.M. (1949) Determination of serum protein by means of the biuret reaction. J. Biol. Chem. 177,751-755.
• 13. Kumick, N.B. (1950) Methylgreen-Pyronin. I. Basis of selective staining of nucleic acids. J. Gen. Physiol. 33,243-274.
• 14. Tsanev, R. (1963) Role of nucleic acids in the wound healing process; in: Regeneration and Wound Healing. Symposium Biologica Huttgarica 3, 55-73.
• 15. Shah, G., Ghosh, R., Amstad, P.A. & Cerutti, P.A. (1993) Mechanism of induction of c-fos by ultra­violet B (290-320 nm) in mouse JB6 epidermal cells. Cancer Res. 53,38-45.
• 16. Dubois, J.-M. & Rouzaire-Dubois, B. (1993) Role of potassium channels in mitogenesis. Prog. Biophys. Molec. Biol. 59,1-21.
• 17. Simon, M.I., Strathmann, M.P. & Gautam, N. (1991) Diversity of G proteins in signal trans­duction. Sdence 252,802-808.
• 18. Tang, W.-J. & Gilman, A.G. (1991) Type-specific regulation of adenylyl cyclase by G-protein subunits. Science 254,1500^-1503.
• 19. Montminy, M.R., Gonzalez, G.A. & Yamamoto, K.K. (1990) Regulation of c-AMP-inducible genes by CREB. Trends Neurosci. 13,184-188.
• 20. Sheng, M., McFadden. G. & Greenberg, M.E. (1990) Membrane depolarization and calcium induce c-fos transcription via phosphorylation of transcription factor CREB. Neuron 4,571-582.
• 21. Colbran, J.L., Roach, P.J., Fiol, C.J., Dixon, J.E., Andrisani, O.M. & Corbin, J.D. (1992) cAMP- -dependent protein kinase, but not cGMP- -dependent enzyme, rapidly phosphorylates A-CREB, and a synthetic A-CRF.B peptide. Biochem. Cell. Biol. 70,1277-1282.
• 22. Bravo, R., Neuberg, M., Burckhardt, J., Almen­dral, J., Wallich, R. & Muller, R. (1987) Invol­vement of common and cell type-specific path­ways in c-fos gene control: Stable induction by cAMP in macrophages. Cell 48,251-260.
• 23. Fisch, T.M., Prywes, R., Simon, M.C. & Roeder, R.G. (1989) Multiple sequence elements in the c-fos promoter mediate induction by cAMP. Genes Deo. 3,198-211.
• 24. Berkovitz, L.A., Riabowol, K.T. & Gilman, M.Z. (1989) Multiple sequence elements of a single functional class are required for cyclic AMP responsiveness of the mouse c-fos promoter. Mol. Cell. Biol. 9,4272-4281.
• 25. Tsuda, T., Fukumoto, Y., Hamamori, Y., Yamashita, T. & Takai, Y. (1987) Involvement of two intracellular messenger systems, protein kinase C and cyclic AMP in the regulation of c-fos expression in human promyelocytic leukemia (HL-60) cells. /. Biochem. (Tokyo) 102, 1579-1583.
• 26. Nakamura, T., Datta, R., Sherman, M.I. & Kufe, D. (1990) Regulation of c-jun gene expression by cAMP in HL-60 myeloid leukemia cells. J. Biol. Chem. 265,22011-22015.
• 27. Hart, I.R., Rao, J. & Wilson, R.E. (1989) c-AMP- -induced c-fos expression in cells of melanocyte origin. Biochem. Biophys. Res. Commun. 159, 408-413.
• 28. Sassone-Corsi, P., Visvader, J., Ferland, L., Mellon, P.L & Verma, I.M. (1988) Induction of proto-oncogene fos transcription through the adenylyl cyclase pathway: Characterization of a cAMP-responsive element. Genes Dev. 2, 1529-1538.
• 29. Goulding, M.D. & Ralph, R.K. (1989) Cyclic AMP-induced c-fos expression and its relevance to differentiation of a transformed mast cell line. Biochim. Biophys. Acta 1007,99-108.
• 30. Hu, J.S. & Olson, E.N. (1988) Regulation of differentiation of the BC3H1 muscle cell line through cAMP-dependent and -independent pathways. J. Biol. Chem. 263,19670-19677.
• 31. Muller, U., Roberts, M.P, Engel, D.A., Doerfler, W. & Schenk, T. (1989) Induction of transcription factor AP-1 by adenovirus El A protein and cAM P. Genes Deo. 3,1991-2002.
• 32. Buscher, M., Rahmsdorf, H., Liffin, J., Karin, M. & Harrlich, P. (1988) Activation of the c-fos gene by UV and phorbolester: different signal trans­duction pathways converge to the same enhancer element. Oncogene 3,301-311.
• 33. Ofir, R., Dwarki, V.J., Rashid, D. & Verma, I.M. (1990) Phosphorylation of theC-terminus of Fos protein is required for transcriptional trans- repression of the c-fos promoter. Nature (London) 348,80-82.
• 34. Abate, C., Marshak, D.R. & Curran,T. (1991) Fos is phosphorylated by p34cdc2, cAMP- -dependent protein kinase and protein kinase C at multiple sites clustered within regulator)' regions. Oncogene 6,2179-2185.
• 35. Dotto, G.P., Gilman, M.Z., Miruyama, M. & Weinberg, R.A. (1986) c-myc and c-fos expression in differentiating mouse primary keratinocytes. EMBOJ. 5,2853-2857.
• 36. Di Fiore, P.P., Falco, J., Borello, I, Weissman, B. & Aaronson, S.A. (1988) The calcium signal for BALB/MK keratinocytes terminal differen­tiation counteracts epidermal growth factor (EGF) very early in the EGF-induced proli­ferative pathway Mol. Cell. Biol. 8,557-563.
• 37. Fisher, C, Byers, M.R., ladarola, M.J. & Powers, E. A. (1991) Patterns of epithelial expression ofFos protein suggest important role in the transition from viable to cornified cell during keratinization. Development 111, 253-258.
• 38. King, K.L. & Cidlowski, J.A. (1995) Cell cycle and apoptosis: Common pathways to life and death. J. Cell. Biochem. 58,175-180'
• 39. Meikrantz, W. & Schlegel, R. (1995) Apoptosis and the cell cycle. /. Cell. Biochem. 58,160-174.
• 40. Pandey, S. & Wang, E. (1995) Cells en route to apoptosis are characterized by the upregulation of c-fos, c-myc, c-jun, cdc2 and RB phospho­rylation, resembling events of early cell-cycle traverse. /. Cell. Biochem. 58,135-150.
• 41. Liu, M. & Pelling, J.C. (1995) UV-B/A irra­diation of mouse keratinocytes results in p53- -mediated WAF1/CIP1 expression. Oncogene 10,1955-1960.
• 42. Haapajarvi, T., Kivinen, L., Pitkanen, K. & Lai ho, M. (1995) Cell cycle dependent effects of u.v.-radiation on p53 expression and retino­blastoma protein phosphorylation. Oncogenes, 151-159.
• 43. Milner, J. (1995) DNA damage, p53 and anti­cancer therapies. Nature Medicine 1,879-880.
• 44. Sewing, A. & Muller, R. (1994) Protein kinase A phosphorylates cyclin D1 at three distinct sites within the cyclin box and at the C-terminus. Oncogene 9,2733-2736.
• 45. Curran, T., Peters, G., Van Beveren, C., Teich, N.M. & Verma, I.M. (1982) FBI murine osteosarcoma virus: Identification and molecular cloning of biologically active pro viral DNA. /. Virol. 44,674-682.
• 46. Shaw, R.J., Doherty, D.F.., Ritter, A.G., Benedict, S.H. & Clark, R.A.F. (1990) Adherence-depen­dent increase in human monocyte PDGF(B) mRNA is associated with increase in c-fos, c-jun and EGR2 mRNA. J. Cell. Biol. Ill, 2139-2148.