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2003 | 08 | 3 |

Tytuł artykułu

The activity of antioxidant enzymes in Arabidopsis thaliana exposed to colchicine and H2O2

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Studies on the possible interference of colchicine and H2O2 with the activity of some antioxidant enzymes were carried out on Arabidopsis thaliana v. Columbia grown in Murashige and Skooge nutrient medium. Measurements of superoxide dismutase (SOD), guaiacol peroxidase (POX), ascorbate peroxidase (APX) and catalase (CAT) activities were conducted spectrophotometrically. In the presence of colchicine, SOD activity increased, while CAT, APX and POX activities decreased. Inhibitory H2O2 effects on the activity of the enzymes were found. Colchicine pre-treatment resulted in an increase in CAT activity and a further increase in SOD activity in plants treated with H2O2.

Wydawca

-

Rocznik

Tom

08

Numer

3

Opis fizyczny

p.777-781,fig.

Twórcy

  • Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland
autor

Bibliografia

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  • 2.Beauchamp, Ch. and Fridovich, I. Superoxide dismutase: improved assay and assay applicable to acrylamide gels. Anal. Biochem. 44 (1971) 276-287.
  • 3.De Marco, A. and Roubelakis-Angelakis, K.A. The complexity of enzymic control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts. Plant Physiol. 110 (1996) 137-145.
  • 4.Ernster, L. and Dallner, G. Biochemical, physiological and medical aspects of ubiquinone function. Biochim. Biophys. Acta 1271 (1995) 195-204.
  • 5.Guan, J., Zhu, X.T., Tachibana, Y., Bastow, K.F., Brossi, A., Hamel, E. and Lee, K.H. Antitumor agents. 185. Synthesis and biological evaluation of tridemethylthiocolchicine analogues as novel topoisomerase II inhibitors. J. Med. Chem. 44 (1998) 1956-1961.
  • 6.Gueta-Dahan, Y., Yani, Z., Zilinskas, B.A. and Ben-Hayyim, G. Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in Citrus. Planta 203 (1997) 460-469.
  • 7.Kliebenstein D.J., Monde R-A. and Last R.L. Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization. Plant Physiol. 118 (1998) 637-650.
  • 8.Milosevic, N. and Slusarenko, A.J. Active oxygen metabolism and lignificationin the hypersensitive response in bean. Physiol. Molec. Plant Pathol. 49 (1996) 1438-1586.
  • 9.Mukherjee, A.K., Basu, S., Sarkar, N. and Ghosh, A.C. Advances in cancer therapy with plant based natural products. Curr. Med. Chem. 8 (2001) 1467-1486.
  • 10.Murashige, T. and Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15 (1962) 473-497.
  • 11.Nakano, Y. and Asada, K. Purification of ascorbate peroxidase in spinach chloroplasts: its inactivation in ascorbate depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol. 28 (1987) 131-140.
  • 12.Orendi, G., Zimmermann, P., Baar, C. and Zentgraf, U. Loss of stress-induced expression of catalase3 during leaf senescence in Arabidopsis thaliana is restricted to oxidative stress. Plant Sci. 161 (2001) 301-314.
  • 13.Wanke, M., Dallner, G. and Świeżewska, E. Subcellular localization of plastoquinone and ubiquinone biosynthesis in spinach cells. Biochim. Biophys. Acta 1463 (2000) 188-194.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-6d54b863-2ef0-46a9-967f-ee1376ce6d38
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