Survey of antimicrobial susceptibility among some pathogenic and commensal bacteria isolated from pigs in Lithuania

• Autorzy: Ruzauskas M , Klimiene I. , Zienius D.
• Języki publikacji: EN

Abstrakty

EN

Antimicrobial susceptibility of isolated enterotoxigenic E. coli, Salmonella enterica serovar Choleraesuis, Pasteurella multocida and Streptococcus suis from pigs was tested in the Veterinary Institute of the Lithuanian Veterinary Academy. Commensal E. coli and Enterococcus faecalis were also included in the testing as commensal bacteria. Clinical and pathological material was investigated from various regions of the country. Isolation and identification of bacteria was done using common methods. The agar diffusion method according to NCCLS guidelines was applied for antimicrobial susceptibility testing. Enterotoxigenic E. coli showed the highest resistance to tetracycline (67%), ampicillin (52%) and sulfamethoxazole-trimethoprim (43%). Not less than 90% of these bacteria were susceptible to colistin, florfenicol and ceftiofur. Salmonella Choleraesuis demonstrated the highest resistance to tetracycline (53%). Florfenicol, ceftiofur and enrofloxacin were effective against most strains of salmonella. Pasteurella multocida in most cases were susceptible to all the tested antimicrobials, however 20% of the isolates were resistant to sulfamethoxazole-trimethoprim. Streptococcus suis demonstrated the highest resistance to tetracycline (43%), lincomycin (40%), sulfamethoxazole- -trimethoprim (40%), and erythromycin (30%). Ceftiofur was the most effective against S. suis. Commensal E. coli showed less resistance than enterotoxigenic E. coli, however not less than 25% of isolates were resistant to tetracycline, sulfamethoxazole-trimethoprim and ampicillin. All the tested Enterococcus faecalis were susceptible to vancomycin and ceftiofur and 80% of enterococci were resistant to tetracycline.

Słowa kluczowe

EN

Lithuania; pig; bacterial resistance; antimicrobial susceptibility; pathogenic bacteria; commensal bacteria; isolation; resistance; pork production; Escherichia coli; Salmonella; Pasteurella multocida; Streptococcus suis; Enterococcus faecalis

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 04
• Opis fizyczny: p.397-400,ref.

Twórcy

autor

Ruzauskas M

• Veterinary Institute of Lithuanian Veterinary Academy, Instituto 2, Kaisiadorys, LT-56115, Lithuania

autor

Klimiene I.

autor

Zienius D.

Bibliografia

• 1.Anon.: Swedish Veterinary Antimicrobial Resistance Monitoring (SVARM). National Veterinary Institute, Uppsala, Sweden 2003.
• 2.Aarestrup F. M. L.: Monitoring of antimicrobial resistance among food animals: principles and limitations. J. Vet. Med. 2004, 51, 3800-3808.
• 3.Aarestrup F. M., Rasmussen S. R., Artursson K., Jensen N. E.: Trends in the resistance to antimicrobial agents of Streptococcus suis isolates from Denmark and Sweden. Vet. Microbiol. 1998, 63, 71-80.
• 4.Baggesen D. L., Sandvang D., Aarestrup F. M.: Characterization of Salmonella enterica serovar Typhimurium DT104 isolated from Denmark and comparison with isolates from Europe and the United States. J. Clin. Microbiol. 2000, 38, 1581-1586.
• 5.Barrigazi G., Candotti P., Foni L., Martinelli L., Raffo A.: Proc. 14th Internat. Pig Vet. Soc. Congress, Bologna, Italy 1997, p. 308.
• 6.Burch D. G. S.: Pharmacokinetics antimicrobial sensitivity and resistance. Pig Journal 2003, 52, 150-165.
• 7.Burch D.: Problems of antibiotic resistance in pigs in the United Kingdom. In Practice 2005, 27, 37-43.
• 8.Bywater R., Deluyker H., Deroover E., deJong A., Marion H., McConville M., Rowan T., Shryock T., Shuster D., Thomas V., Vale M., Walters J.: A European survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing animals. J. Antimicrob. Chemother. 2004, 54, 744- -754.
• 9.Daignault D., Higgins R., Messier S., CoutureY.: Sensibilité des isolats de Pasteurella multocida et Pasteurella haemolytica envers différents agents antibactériens. Méd. Vét. Québec 1997, 27, 154-155.
• 10.Del'Arco A. E., Guimarães W. V.: Resistance and sensitivity of Streptococcus suis to drugs. Proc. 17th Internat. Pig Vet. Soc. Congress, USA 2002, p. 335.
• 11.Guerra B., Junker E., Schroeter A., Malorny B., Lehmann S., Helmuth R.: Phenotypic and genotypic characterization of antimicrobial resistance in German Escherichia coli isolates from cattle, swine and poultry. J. Antimicrob. Chemother. 2003, 52, 489-492.
• 12.Hariharan H., Coles M., Poole D., Page R.: Antibiotic resistance among enterotoxigenic Escherichia coli from piglets and calves with diarrhea. Can. Vet. J. 2004, 45, 605-606.
• 13.Hoszowski A., Wasyl D.: Salmonella prevalence and resistance to antibiotics in Poland. Medycyna Wet. 2005, 61, 660-663.
• 14.Hoszowski A., Wasyl D.: Salmonella serovars found in animals and feeding stuffs in 2001 and their antimicrobial resistance. Bull. Vet. Inst. Pulawy 2002, 46, 165-178.
• 15.King S. J., Leigh J. A., Heath P. J., Luque I., Tarradas C., Dowson C. G., Whatmore A. M.: Development of a multilocus sequence typing scheme for the pig pathogen Streptococcus suis: identification of virulent clones and potential capsular serotype exchange. J. Clin. Microbiol. 2002, 40, 3671-3680.
• 16.Mathew A. G., Saxton A. M., Upchurch W. G., Chattin S. E.: Multiple antibiotic resistance patterns of Escherichia coli isolates from swine farms. Appl. Environ. Microbiol. 1999, 65, 2770-2772.
• 17.Maynard C., Fairbrother J. M., Bekal S., Sanschagrin F., Levesque R. C., Brousseau R., Masson L., Larivière S., Harel J.: Antimicrobial resistance genes in enterotoxigenic Escherichia coli O149:K91 isolates obtained over a 23 year period from pigs. Antimicrob. Agents Chemother. 2003, 47, 3214-3221.
• 18.Mølbak K., Gerner-Smidt P., Wegener H. C.: Increasing quinolone resistance in Salmonella enterica serotype Enteritidis. Emerg. Infect. Dis. 2002, 8, 514-519.
• 19.Morvan H.: Sensibilité aux antibiotiques en élevage industriel. Institut Supérieur des Productions animales et des Industries Agro-alimentaires, Ploufragan, France 1994, p. 209-222.
• 20.Nadeau M., Coté G., Higgins R.: Surveillance de l’antibiorésistance chez des bactéries d’origine aviaire et porcine de 1993 à 1999 au Québec. Méd. Vét. Québec 2001, 30, 1995-1999.
• 21.Reams R. Y., Glickman L. T., Harrington D. D., Bowersock T. L., Thacker H. L.: Streptococcus suis infection in swine: a retrospective study of 256 cases. Part I. Epidemiologic factors and antibiotic susceptibility patterns. J. Vet. Diag. Invest. 1993, 5, 363-367.
• 22.Ruzauskas M., Amrozevièienë È. B.: Most common pathogenic bacteria and their antimicrobial resistance in pigs. Þemës ûkio mokslai 2004, 2, 44-51.
• 23.Ruzauskas M., Virgailis M., Spakauskas V.: Serological diversity and antimicrobial resistance of Salmonella isolated from different sources in Lithuania. Vet. Arhiv 2005, 75, 211-221.
• 24.Wasyl D., Hoszowski A.: Antibiotic susceptibility in Salmonella swine isolates. Salinpork, 4th International Symposium on the epidemiology and control of Salmonella and other food borne pathogens in pork. Leipzig, Germany 2001.
• 25.Williams J. J., Salazar F. M. R., Ramírez P. R., Mosqueda A. Z.: Sensibilidad in vitro de cepas de Actinobacillus pleuropneumoniae y Pasteurella multocida tipo ”A” ante diferentes antimicrobianos. Rev Biomed. 2001, 12, 172-179.