Effects of tyrosine kinase inhibitors on voltage-dependent BA2+ currents in the guinea-pig gastric antrum

• Autorzy: Zhu H.L. , Ito Y. , Teramoto N.
• Języki publikacji: EN

Abstrakty

EN

We have investigated whether tyrosine kinases modify the activity of voltage-dependent Ba2+ currents (IBa) recorded from guinea-pig gastric myocytes by use of patch-clamp techniques. All experiments were carried on single smooth muscle cells, dispersed from the circular layer of the guinea-pig gastric antrum. Genistein (10 µM), a specific tyrosine kinase inhibitor, reduced the peak amplitude of IBa in a voltage- and concentration-dependent manner. Daidzein (30 µM), an inactive analog of genistein, also inhibited IBa in a concentration-dependent manner. Similarly, other types of tyrosine kinase inhibitors (lavendustin A and tyrphostin 23) suppressed the peak amplitude of IBa in a concentration-dependent manner. These results indicate that tyrosine kinases may be essential to regulate Ca2+ mobilization through voltage-dependent Ca2+ channels in gastric myocytes.

Słowa kluczowe

EN

calcium channel; gastric smooth muscle; gastric myocyte; gastric antrum; guinea pig; tyrosine kinase; inhibitor

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2006
• Tom: 57
• Numer: 3
• Opis fizyczny: p.415-424,fig.,ref.

Twórcy

autor

Zhu H.L.

• Kyushu University, Fukuoka, Japan

autor

Ito Y.

autor

Teramoto N.