Filogenetyczne pokrewienstwo ulicznych szczepow wirusa wscieklizny a reaktywnosc ich antygenow z przeciwcialami indukowanymi szczepem szczepionkowym. I. Ocena pokrewienstwa szczepow wirusa wscieklizny izolowanych w Polsce

• Autorzy: Sadkowska-Todys M.
• Języki publikacji: PL

Abstrakty

PL

Charakterystyki szczepów dokonano na podstawie analizy sekwencji nukleotydów odcinka genu nukleoproteiny wirusa wścieklizny o długości 400 par zasad, zlokalizowanego na końcu aminowym białka N (nukleoproteina). Przeprowadzone badania wykazały zróżnicowanie genetyczne szczepów izolowanych od zwierząt naziemnych na terenie Polski. Ustalono, że szczepy genotypu 1 izolowane na terenie kraju należą do czterech grup filogenetycznych: NEE, CE, WE i EE występujących na terenie Europy. Określono także pokrewieństwo filogenetyczne pomiędzy referencyjnymi szczepami laboratoryjnymi i polskimi szczepami ulicznymi genotypu 1 i 5 wirusa wścieklizny.

EN

The aims of these studies were: genetic characteristic of street rabies virus strains isolated from different animal species in Poland and determination of phylogenetic relationships to reference laboratory strains of the street rabies viruses belonging to genotype 1 and 5. The variability of rabies isolates and their phylogenetic relationship were studied by comparing the nucleotide sequence of the virus genome fragment. The Polish strains of genotype 1 belong to four phylogenetic groups (NE, CE, NEE, EE) corresponding to four variants: fox - racoon dog (F-RD); European fox 1 (F1); European fox 2 (F2) and European fox 3 (F3). On the Polish territories there are no rabies strains representing the variant dog-wolf and typical for arctic fox variant. The similarity of nucleotide and amino acid sequences of street rabies strains belonging to genotype 1 and laboratory strain CVS is very high. It is about 91% similarity at nucleotide level and 95% at amino acid level. Rabies strain CVS is similar to genotype 5 bat strains (EBL 1) only in about 69% and 74% at nucleotide and amino acid level, respectively. The genetic divergence of rabies strains circulating in Poland raised the need of pernament epidemiological and virological surveillance. The genotype and variant of isolated strains should be determined (using PCR and RLFP methods).

Słowa kluczowe

PL

wirus wscieklizny; pokrewienstwo filogenetyczne; zroznicowanie genetyczne; szczepy uliczne; genotyp; szczepy laboratoryjne; wirusy; szczepy wirusowe; Polska; wscieklizna

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2000
• Tom: 52
• Numer: 2
• Opis fizyczny: s.173-183,rys.,tab.,bibliogr.

Twórcy

autor

Sadkowska-Todys M.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

Bibliografia

• 1. Amengual B, Whitby JE, King A i inni. Evolution of European bat lyssaviruses. J Gen Virol 1997; 78: 2319-28.
• 2. Bourhy H, Kissi B, Lafon M, Sacramento D i inni. Antigenic and molecular characterization of bat rabies virus in Europe. J Clin Microbiol 1992; 30: 2419-26.
• 3. Bourhy H, Kissi B, Tordo N. Molecular diversity of the Lyssavirus genus. Virology 1993; 194: 70-81.
• 4. Bourhy H, Kissi B, Tordo N. Taxonomy and evolutionary studies on Lyssaviruses with special reference to Africa. Onderstepoort J Vet Res 1993; 60: 277-82.
• 5. Bourhy H, Kissi B, Tordo N i inni. Molecular epidemiological tools and phylogenetic analysis of bacteria and viruses with special emphasis on lyssaviruses. Prev Vet Med 1995; 25: 161-81.
• 6. Bourhy H, Kissi, B, Audry L i inni. Ecology and Evolution of rabies viruses in Europe. J Gen Virol 1999; 80: 2545-58.
• 7. Clark HF, Wiktor TJ. Rabies virus. Strains of human viruses. Red. M. Majer, S.A. Plotkin, Karger, Basel 1972, 177-82.
• 8. Higgins DG, Sharp PM. Fast and sensitive multiple sequence alignments on a microcompu#ter. CABIOS 1989; 5: 151-3.
• 9. Kissi B, Tordo N, Bourhy H. Genetic polymorphism in the rabies virus nucleoprotein gene. Virology 1995; 209: 526-37.
• 10. Muller WW. New Lyssavirus in fruit bats in Australia. WHO Rabies Bull Eur 1996; 20: 9-10.
• 11. Nadin-Davis SA, Casey GA, Wandeler A. Identification of regional variants of the rabies virus within the Canadian province of Ontario. J Gen Virol 1993; 74: 829-37.
• 12. Sacramento D, Bourhy H, Tordo N. PCR technique as an alternative method for diagnosis and molecular epidemiology of rabies virus. Mol Cel Prob 1991; 5: 229-40.
• 13. Sacramento D, Badrane H, Bourhy H i inni. Molecular epidemiology of rabies virus in France; comparison with vaccine strains. J Gen Virol 1992; 73: 1149-58.
• 14. Saitou N, Nei M. The neighbour joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406-25.
• 15. Seroka D. The distribution of stationary foci of rabies in wild animals in Poland Epid Rev 1968 22: 1968. 66-75.
• 16. Smith JS, Orciari LA, Yager PA i inni. Epidemiologic and historical relationship among 87 rabies virus isolates as determined by limited sequence analysis. J Infect Dis 1992; 166: 296-307.
• 17. Smith JS, Orciari LA, Yager PA. Molecular epidemiology of rabies in the United States.Virology 1995; 6: 387-400.
• 18. Smith JS. New aspects of rabies with emphasis on epidemiology, diagnosis, and prevention of the disease in the United States. Clin Microbiol Rev 1996; 9: 166-76.
• 19. Teichman BF, Thomson GR, Meredith CD i inni. Molecular epidemiology of rabies virus in South Africa: evidence for two distinct virus group. J Gen Virol 1995; 76: 73-82.