Purification and characterization of extracellular Pseudomonas aeruginosa urate oxidase enzyme

• Autorzy: Saeed H M , Abdel-Fattahyousry Y.R. , Gohar M. , Elbaz M.A.
• Języki publikacji: EN

Abstrakty

EN

Urate oxidase (uricase) was isolated and purified from Pseudomonas aeruginosa to apparent homogeneity using ammonium sulphate precipitation followed by ion exchange and gel filtration chromatography. The specific activity of the purified uricase enzyme was found to be 636.36 with the use of uric acid as a substrate. The purified uricase enzyme is a monomeric protein with molecular weight of 64 kilodaltons. The optimal pH and temperature of the purified enzyme is 9.0 and 30°C, respectively. The effect of some metal ions was studied. Sulphate forms of Fe⁺², Zn⁺² and Co⁺² inhibit the uricolytic activity whereas; NaCl and CaCl₂ enhance the enzyme activity. Moreover, the purified enzyme is inhibited by EDTA and KCN.

Słowa kluczowe

EN

gel filtration chromatography; ammonium sulphate; enzyme; Pseudomonas aeruginosa; uric acid; purification; uricase enzyme; urate oxidase; ion exchange

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: 1
• Opis fizyczny: p.45-52,fig.,ref.

Twórcy

autor

Saeed H M

• University of Alexandria, Chatby 21526, Alexandria, Egypt

autor

Abdel-Fattahyousry Y.R.

autor

Gohar M.

autor

Elbaz M.A.

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