Nucleotide sequence of the coat protein gene of the Skierniewice isolate of plum pox virus [PPV]

• Autorzy: Wypijewski K. , Malinowski T. , Musial W. , Augustyniak J.
• Języki publikacji: EN

Abstrakty

EN

The coat protein (CP) gene of the Skierniewice isolate of plum pox virus (PPV-S) has been amplified using the reverse transcription — polymerase chain reaction (RT-PCR), cloned and sequenced. The nucleotide sequence of the gene and the deduced amino-acid sequence of PPV-S CP were compared with those of other PPV strains. The nucleotide sequence showed very high homology to most of the published sequences. The motif: Asp-Ala-Gly (DAG), important for the aphid transmissibility, was present in the amino-acid sequence. Our isolate did not react in ELISA with monoclonal antibodies MAb06 supposed to be specific for PPV-D

Słowa kluczowe

EN

plum; Skierniewice; reverse transcription; Poland; polymerase chain reaction; fruit tree; pox virus; nucleotide sequence; coat protein gene

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1994
• Tom: 41
• Numer: 1
• Opis fizyczny: p.87-95,fig.

Twórcy

autor

Wypijewski K.

• A.Mickiewicz University, ul.Fredry 10, 61-701 Poznan, Poland

autor

Malinowski T.

autor

Musial W.

autor

Augustyniak J.

Bibliografia

• 1. Nemeth, M. (1986) Plum pox (Sharka); in Virus, Mycoplasma and Rickettsia Diseases of Fruit Trees; pp. 463-479, Akademiai Kiado, Budapest.
• 2. Kegler, H. & Shade, Ch. (1970) Plum Pox Virus; C.M.I. A.A.B. Descriptions of Plant Viruses. No. 70
• 3. Wand, C.W. & Shukla, D.D. (1991) Taxonomy of potyviruses: current problems and some solu­tions. Intervirology 32, 269-2%.
• 4. Riechmann, J.L., Lain, S. & Garcia, J.A. (1992) Highlights and prospects of potyvirus mole­cular biology. J. Gen. Virol. 73,1-16.
• 5. Shukla, D.D., Frenkel, M.J. & Ward, C.W. (1991) Structure and function of the potyvirus genome with special reference to the coat protein coding region. Gmad. /. Plant Pathol. 13,178-191.
• 6. Wetzel, T., Candresse, T., Ravelonandro, M., Delbos, R.P., Mazyad, H., Aboul-Ata, A.E. & Dunez, J. (1991) Nucleotide sequence of the 3' terminal region of the RNA from an atypical strain (El Amar) of plum pox potyvirus. /. Gen. Virol. 72,1742-1746.
• 7. Szczygieł, A. (1962) Pojawienie się w Polsce groźnej choroby wirusowej szarki Primus virus 7. (Appearence a severe viral disease sharka Prunus virus 7 in Poland). Ochrona Roślin 6, 14-17.
• 8. Zawadzka, B. (1980) The response of several plum cultivars to infection with plum pox virus. Acta Phytopathol. Acad. Sci. Hungar. 15,215-221.
• 9. Zawadzka, B. & Malinowski, T. (1989) Response of a few peach cultivars to infection with plum pox vims. Plant Virology Proceedings of the 10th Conference of the Czechoslovak Plant Virologist, 213.
• 10. Ling, K., Namba, S., Gonsalves, C., Slightom, J.L. & Gonsalves, D. (1991) Protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the papa­ya ringspot virus coat protein gene. Biotech­nology 9,752-75$.
• 11. Gonsalves, D., Chee, P., Prowidenti, R., Seem, R. & Slightom, J.L. (1992) Comparison of coat protein-mediated and genetically-derived resistance in cucumbers to infection by cucum­ber mosaic virus under field conditions with natural challenge inoculations by vectors. Biotechnology 10,1562-1570.
• 12. Wilson, T.M.A. (1993) Strategies to protect crop plants against viruses: pathogen derived resis­tance blossoms. Proc. Natl. Acad. Sci. U.S.A. 90, 3134-3141.
• 13. Maiss, E., Breyel, E., Brisske, A. & Casper, R. (1988) Molecular cloning of DNA comple­mentary to the RNA genome of plum pox virus (PPV). /. Phytopathol. 122,222-231.
• 14. Ravelonandro, M., Varveri, C., Delbos, R. & Dunez, J. (1988) Nucleotide sequence of the capsid protein gene of plum pox potyvirus. /. Gen. Virol. 69,1509-1516.
• 15. Moghal, S.M. & Francki, R.I.B. (1976) Towards a system for the identification and classification of Potyviruses. Virology 73,350-362.
• 16. Navratil, M., Cikanek, D., Hilgert, I., Kristofova, H. & Karesova, R. (1992) Monoclonal antibodies against plum pox virus. Acta Horticulturae 309, 169-173.
• 17. Hilgert, I., Cikanek, D., Kristofova, H., Kare­sova, R. & Navratil, M. (1992) Monoclonal anti­bodies suitable for plum pox virus determi­nation. Hybridoma (in press).
• 18. Kerlan, C. & Dunez, J. (1979) Differentiation biologique et serologique de souches du virus de la Sharka. A?m. Phytopathol. 11, 241-250.
• 19. Clark, M.F. & Bar-Joseph, M. (1984) Plant virus enzyme immunosorbent assays. Methods Virol. 7,60-61.
• 20. Lain, S., Riechmann, J.L. & Garcia, J.A. (1989) The complete nucleotide sequence of plum pox potyvirus RNA. Virus Res. 13,157-172.
• 21. Teycheney, P.Y., Tavert, G., Delbos, R., Rave­lonandro, M. & Dunez, J. (1989) The complete nucleotide sequence of plum pox virus RNA (strain D). Nucl. Acids Res. 17,10115-10116.
• 22. Grouse, G.F., Frischauf, A. & Lehrach, H. (1983) An integrated and simplified approach to cloning into plasmids and single-stranded phages. Methods Enzymol. 101,78-89.
• 23. Hanahan, D. (1985) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
• 24. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) in Molecular clotting. A laboratory manual, pp. 1.25, Cold Spring Harbor Laboratory Press.
• 25. Gatger, S.J., Griffith, O.M. & Grill, L.K. (1974) Rapid purification of plasmid DNA by a single centrifugation in a two-step caesium chloride ethidium-bromide gradient. Technical Bulletin of Beckman, G-594.
• 26. Sanger, F., Nicklen, S. & Coulson, A.R. (1977) DNA sequencing with chain-termination inhi­bitors. Proc. Natl. Acad. Sci. U.S.A. 74,5463-5467.
• 27. Wetzel, T., Candresse, T., Ravelonandro, M. & Dunez,J. (1991) A polymerase chain reaction adapted to plum pox potyvirus detection. J. Virol. Meth. 33,355-356.
• 28. Maiss, E.,Hmmpe, U., Brisske, A.,Jelkmann, W., Casper, R., Himmler, G., Mattanovich, D. & Katinger, H.W.D. (1989) The complete nucleo­tide sequence of plum pox virus RNA. J. Gen. Virol. 70,513-524.
• 29. Ward, C.W., McKern, N.M., Frenkel, M.J. & Shukla, D.D. (1992) Sequence data as the major criterion for potyvirus classification. Arch. Virol. (Suppl. 5) 283-297.
• 30. Wetzel, T., Candresse, T., Macquaire, G., Ravelonandro, M. & Dunez, J. (1992) A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus dete­ction. /. Virol. Meth. 39,27-37.
• 31. Smolarz, S. & Zawadzka, B. (1978) Skuteczność mszyc w przenoszeniu wirusa ospowatości śliwy (szarki). (Efficiency of aphids in trans­mission of plum pox (sharka) virus). Zesz. Probl. Post. Nauk Roln. 214,51-58.
• 32. Cervera, M.T., Riechmann, J.L., Martin, M.T. & Garcia, J.A. (1993) 3'-Terminal sequence of the plum pox virus PS and oS isolates: evidence for RNA recombination within the potyvirus group. /. Gen. Virol. 74,329-334.