Calcium overload increases oxidative stress in old rat gastrocnemius muscle

• Autorzy: Capel F. , Demaison L. , Maskouri F. , Diot A. , Buffiere C. , Patureau Mirand P. , Mosoni L.
• Języki publikacji: EN

Abstrakty

EN

In order to challenge in vivo muscle Ca2+ homeostasis and analyze consequences on mitochondrial H2O2 release (MHR) and sarcopenia, we injected Ca2+ ionophore A23187 (200 µg/kg, ip) in adult and old rats and measured gastrocnemius mass and mitochondrial Ca2+ content (MCC) using radioactive Ca2+ 48 h after injection. In a second experiment performed in old rats, we measured isocitrate dehydrogenase (ICDH) activity as an index of MCC, MHR, mitochondrial respiration, citrate synthase, COX and antioxydant enzyme activities 24 h after a 150 µg/kg injection. In adult rats, muscle mass and MCC were unchanged by A23187. In old rats, MCC increased 24 h after injection as reflected by a significant increase in ICDH activity; measured MCC tended to increase at 48 h. MHR and Mn-SOD activity were significantly increased at 24 h, and GPX activity was reduced. Muscle mass was unchanged but was negatively correlated with MCC in control and treated old rats. In conclusion, in old rats, A23187 probably induced a mitochondrial Ca2+ overload responsible for the observed increase in MHR without leading to muscle atrophy on a short term basis.

Słowa kluczowe

EN

calcium; muscle; mitochondrion; gastrocnemius muscle; aging; oxidative stress; free radical; rat; skeletal muscle

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2005
• Tom: 56
• Numer: 3
• Opis fizyczny: p.369-380,fig.,ref.

Twórcy

autor

Capel F.

• Universite J.Fourier, BP 53, 38041 Grenoble Cedex 09, France

autor

Demaison L.

autor

Maskouri F.

autor

Diot A.

autor

Buffiere C.

autor

Patureau Mirand P.

autor

Mosoni L.