Pyoverdine production in Pseudomonas fluorescens UTPF5 and its association with suppression of common bean damping off caused by Rhizoctonia solani [Kuhn]

• Autorzy: Sharifi R , Ahmadzadeh M. , Sharifi-Tehrani A. , Talebi-Jahromi K.

Warianty tytułu

PL

Wytwarzanie pyowerdyny przez Pseudomonas fluorescens UTPF5 i jej zwiazek z ograniczaniem zgorzeli fasoli zwyczajnej wywolywanej przez Rhizoctonia solani

• Języki publikacji: EN

Abstrakty

EN

Siderophore production is an important mechanism of biological control by a number of strains of plant growth-promoting rhizobacteria. Pseudomonas fluorescens UTPF5 was originally obtained from onion field. Biochemical and physiological characteristics of this strain refer to biovar 3 of P. fluorescens. Strain UTPF5 is an effective bacterium against several phytopathogenic fungi. Pyoverdine type siderophore of this strain was isolated using XAD amberlite column. The plant growth promotion and antifungal properties of bacteria were demonstrated under greenhouse conditions in combination with Fe-EDTA, Fe-EDDHA and Zn as modulators of pyoverdine production. Amendment with zinc, Fe-EDTA and Fe-EDDHA suppressed the disease inhibition when partially used with UTPF5. 7NSK2 and its pyoverdine mutant, MPFM1, were used as reference strains the inhibition percent of which was not affect by soil amendment. Iron chelates, especially Fe-EDDHA, increased growth and chlorophyll production by plants. This effect was improved in the presence of bacterial strains. The siderophore mutant MPFM1 did not exhibit satisfactory disease inhibition and growth promotion activity. In vitro experiments showed that purified pyoverdine could decrease the fungal growth to the same extent as pyoverdine-producing strain.

PL

Wytwarzanie syderoforów jest ważnym mechanizmem biologicznego zwalczania przez wiele szczepów bakterii rizosferowych stymulujących wzrost roślin. Pseudomonas fluorescens UTPF5 uzyskano z pola, na którym uprawiano cebulę. Cechy biochemiczne i fizjologiczne tego szczepu znajdują odniesienie do browaru 3 P. fluorescens. Szczep UTPF5 jest bakterią efektywną przeciwko kilku patogenom roślin. Syderofor tego szczepu typu pyowerdyny wyosobniono przy wykorzystaniu kolumny amberlitowej. Pobudzenie wzrostu roślin i przeciwgrzybowe właściwości bakterii stwierdzono w warunkach szklarniowych, wykorzystując Fe-EDTA, Fe-EDDHA i cynk, jako czynniki modulujące wytwarzanie pyowerdyny. Dodatek cynku, Fe-EDTA i Fe-EDDHA ograniczały inhibicję, gdy były użyte częściowo z UTPF5. 7NSK2 i jego mutant pyowerdynowy MPFM1, zostały użyte jako szczepy porównawcze, w przypadku których, procent inhibicji nie był modyfikowany przez dodatki wprowadzone do ziemi. Chylaty żelaza, specjalnie F-EDDHA, stymulowały wzrost i wytwarzanie chlorofilu przez rośliny. Ten efekt był lepszy w obecności szczepów bakterii. Mutant syderoforowy MPFM1 nie wykazywał zadawalającej inhibicji choroby i stymulującego działania. Doświadczenia in vitro wykazały, że oczyszczona pyowerdyna mogłaby zwiększać wzrost grzyba do takiego samego stopnia jak szczep wytwarzający pyowerdynę.

Słowa kluczowe

EN

Pseudomonas fluorescens; pyoverdin production; common bean; damping off; Rhizoctonia solani; biological control

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2010
• Tom: 50
• Numer: 1
• Opis fizyczny: p.72-78,ref.

Twórcy

autor

Sharifi R

• College of Agriculture, University of Tehran, Karaj, Iran

autor

Ahmadzadeh M.

autor

Sharifi-Tehrani A.

autor

Talebi-Jahromi K.

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