Identification, cloning and expression of Pseudomonas aeruginosa Ps-x putative urate oxidase gene in Escherichia coli

• Autorzy: Saeed H M , Abdel-Fattah Y.R. , Berekaa M.M. , Gohar Y.M. , Elbaz M.A.
• Języki publikacji: EN

Abstrakty

EN

In a previous study we reported for the first time the isolation and characterization of urate oxidase enzyme from Pseudomo-nas aeruginosa. In this work we isolated and cloned a 1.350 kilobase DNA fragment that encode a putative urate oxidase gene from the genomic library of P. aeruginosa Ps-x. The nucleotide sequence of the cloned DNA insert revealed an open reading frame that encodes a protein of a molecular weight of 54.0 kDa. The cloned DNA fragment showed an uricolytic activity when expressed in E. coli DH5α . Surprisingly, the nucleotide sequence of the cloned gene showed more than 99% identity to the gene encoding hypothetical protein of P. aeruginosa PAO1. Moreover, the sequence of the cloned gene was closely similar to the corresponding unease gene of Cellulomonas Jlavigena (44% similarity), but showed lower similarity values to that of Bacillus sp. BT-90 (24% similarity), Candida utilis (24% similarity). Interestingly, the isolated uricase gene showed closer similarity to uricase from yeast-like symbiotic fungi Beauveria bassiana (35%), Tolypocladium inflatum (29%), Paecilomyces tenuipes (27%) and Cerataphis fransseni (24%).

Słowa kluczowe

EN

gene; cloning; enzyme; Pseudomonas aeruginosa; Escherichia coli; expression; urate oxidase; identification

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: 4
• Opis fizyczny: p.227-236,fig.,ref.

Twórcy

autor

Saeed H M

• University of Alexandria, Chatby 21526, Alexandria, Egypt

autor

Abdel-Fattah Y.R.

autor

Berekaa M.M.

autor

Gohar Y.M.

autor

Elbaz M.A.

Bibliografia

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