The effects of thymomimetic drugs and zinc supplementation on viability of hydrocortisone-treated mouse thymocytes

• Autorzy: Obminska-Domoradzka B. , Deboowy J.
• Języki publikacji: EN

Abstrakty

EN

The studies in vitro were carried out on isolated thymic cells of Balb/c mice. The thymocyte cultures were incubated with calf thymus extract (TFX) and diethyldithiocarbamate (DTC) at a concentrations of 0.1-100 μg/ml or zinc ions (as sulphate salt) at a concentration of 1 μg/ml, and next the latter culture was supplemented with hydrocortisone at a concentration of 50 μg/ml/culture. The results of the study show that hydrocortisone supplementation decreases the survival rate of cells by approximately 50% in relation to the control. Preincubation of thymocytes with TFX or DTC perse and in zinc interactions increases the percentage of viable thymocytes, as it results in the induction of differentiation and maturation of the cells under investigation. The preventive action of the two drugs against the thymolythic effect of hydrocortisone depends on TFX and DTC concentrations, the presence of zinc ions and length of incubation. A stronger preventive effect against the cytotoxic impact of hydrocortisone has been observed with TFX than with DTC. The effect of steroid resistance of thymocytes induced by the two thymomimetic drugs is enhanced by zinc ions.

Słowa kluczowe

EN

trace element; viability; zinc ion; mouse; mammal; mice; zinc supplementation; immune system; thymocyte; hydrocortisone; thymomimetic drug

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2000
• Tom: 03
• Numer: 4
• Opis fizyczny: p.231-237

Twórcy

autor

Obminska-Domoradzka B.

• Agricultural University, Norwida 31, 50-375 Wroclaw, Poland

autor

Deboowy J.