Zastosowanie metody AFLP do oceny genetycznego podobienstwa szczepow Bordetella pertussis, Bordetella parapertussis oraz Bordetella bronchiseptica

• Autorzy: Mosiej E. , Gzyl A. , Slusarczyk J.

Warianty tytułu

EN

Genetic diversity analysis of isolates belonging to Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica species

• Języki publikacji: PL

Abstrakty

PL

Technikę AFLP (Amplified Fragment Length Polymorphism) zastosowano do identyfikacji gatunku і do oceny wewnątrzgatunkowej zmienności szczepów B. pertussis, В. parapertussis oraz В. bronchiseptica. Wykazano przydatność metody AFLP do określania gatunkowej przynależności szczepów B. pertussis, В. parapertussis і В. bronchiseptica oraz wewnątrzgatunkowego różnicowania szczepów B. pertussis i B. bronchiseptica.

EN

In this study, Amplified Fragment Length Polymorphism (AFLP) method was used to track differences among human and animal isolates of B. pertussis, B. parapertussis and B. bronchiseptica species. One hundred and sixty representative strains of these species orginated from international and Polish bacterial collections were genotyped according to AFLP involving EcoRl/Msel and Spel/Apal restriction/ligation/amplification procedures. This study has confirmed high potential AFLP Spel/Apal procedure for intra-species differentiation of B. pertussis and B. bronchiseptica strains. Both AFLP EcoRl/Msel and Spel/Apal procedures have been found to be useful for species-specific classification in case of B. pertussis strains. In case of B. bronchiseptica or B. parapertussis species-specific classification, Spel/Apal procedure has been found more precise than EcoRl/Msel one.

Słowa kluczowe

PL

szczepy bakteryjne; Bordetella pertussis; Bordetella parapertussis; Bordetella bronchiseptica; podobienstwo genetyczne; zroznicowanie wewnatrzgatunkowe; oznaczanie; metoda AFLP

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 2
• Opis fizyczny: s.143-153,rys.,tab.,bibliogr.

Twórcy

autor

Mosiej E.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

autor

Gzyl A.

autor

Slusarczyk J.

Bibliografia

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