The induction of apoptosis by daunorubicin and idarubicin in human trisomic and diabetic fibroblasts

• Autorzy: Dragojew S , Marczak A. , Maszewski J. , Ilnicki K. , Jozwiak Z.
• Języki publikacji: EN

Abstrakty

EN

In this study, we investigated apoptosis induced in human trisomic and diabetic fibroblasts by daunorubicin (DNR) and its derivative, idarubicin (IDA). The cells were incubated with DNR or IDA for 2 h and then cultured in a drug-free medium for a further 2–48 h. The apoptosis in the cultured cell lines was assessed by biochemical analysis. We found that both drugs induced a timedependent loss of mitochondrial membrane potential, and a significant increase in intracellular calcium and caspase-3 activity. Mitochondrial polarization and changes in the level of intracellular calcium were observed during the first 2–6 h after drug treatment. Caspase-3 activation occurred in the late stages of the apoptotic pathway. Our findings also demonstrated that idarubicin was more cytotoxic and more effective than daunorubicin in inducing apoptosis in trisomic and diabetic fibroblasts.

Słowa kluczowe

EN

Down's syndrome; diabetes; fibroblast; apoptosis; induction; daunorubicin; idarubicin; caspase-3; chemotherapeutic agent

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 2
• Opis fizyczny: p.182-194,fig.,ref.

Twórcy

autor

Dragojew S

• University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

autor

Marczak A.

autor

Maszewski J.

autor

Ilnicki K.

autor

Jozwiak Z.

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