Application of flow cytometry for the study of plant genomes

• Autorzy: Dolezel J
• Języki publikacji: EN

Abstrakty

EN

During the last fifteen years, flow cytometry and sorting greatly contributed to the improvement of our knowledge of plant genome structure and function. This paper reviews the applications of flow cytometry for the analysis of isolated nuclei and chromosomes. Because of its speed, precision and convenience, this method of analysis of the nuclear DNA content finds an enormous number of applications which cover basic research, breeding and production. The results obtained with chromosome analysis and sorting indicate that the technique might greatly simplify the analysis of plant genomes at the molecular level.

Słowa kluczowe

EN

ploidy; DNA content; chromosome; blood cell; flow karyotype; optical detector system; flow cytogenetics; flow cytometry; genome; nucleus; plant

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 3
• Opis fizyczny: p.285-302,fig.

Twórcy

autor

Dolezel J

• Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Sokolovska 6, CZ-77200 Olomouc, Czech Republic

Bibliografia

• Afza R., Kämer D., Doležel J., König J., van Duren M., Kahl G., Novák F.J. (1992). The potential of nuclear DNA flow cytometry and DNA fingerprinting for Musa improvement programmes. In: Proc. Int. Symp. Breeding Banana and Plantain for Resistance to Diseases and Pests (B. Wills ed.). CIRAD, Montpellier: 65-75.
• Arumuganathan K., Martin G.B., Telenius H., Tanksley S.D., Earle E.D. (1994). Chromosome 2-specific DNA clones from flow-sorted chromosomes of tomato. Mol. Gen. Genet. 242: 551-558.
• Awoleye K., van Duren M., Doležel J., Novák F.J. (1994). Nuclear DNA content and in vitro induced somatic polyploidization in cassava (Manihot esculenta Crantz) breeding. Euphytica 76: 195-202.
• Baranyi M., Greilhuber J. (1995). Flow cytometric analysis of genome size variation in cultivated and wild Pisum sativum (Fabaceae). PI. Syst. Evol. 194: 231-239.
• Baranyi M., Greilhuber J. (1996). Flow cytometric and Feulgen densitomctric analysis of genome size variation in Pisum. Theor. Appl. Genet. 92: 231-239.
• Bashir A., Auger J.A., Rayburn A.L. (1993). Flow cytometric DNA analysis of wheat-rye addition lines. Cytometry 14: 843-847.
• Bennett M.D. (1985). Intraspecific variation in DNA amount and the nucleotypic dimension in plant genetics. In: Plant Genetics (Freeling M., ed.). Alan R. Liss, New York: 283-302.
• Bennett M.D., Leitch I.J. (1995). Nuclear DNA amounts in angiosperms. Ann. Bot. 76: 113-176.
• Bergounioux C., Brown S.C., Petit P.X. (1992). Flow cytometry and plant protoplast cell biology. Physiol. Plant. 85: 374-386.
• Bharathan G., Lambert G., Galbraith D.W. (1994). Nuclear DNA content of monocotyledons and related taxa. Am. J. Bot. 81: 381-386.
• Binarová P., Doležel J. (1988). Alfalfa embryogcnic cell suspension culture: Growth and ploidy level stability. J. Plant Physiol. 133: 561-566.
• Biradar D.P., Rayburn A.L. (1993). Heterosis and nuclear DNA content in maize. Heredity 71: 300-304.
• Biradar D.P., Bullock D.G., Rayburn A.L. (1994). Nuclear DNA amount, growth, and yield parameters in maize. Theor. Appl. Genet. 88: 557-560.
• Biradar D.P., Pedersen W.L., Rayburn A.L. (1994). Nuclear DNA analysis of maize seedlings treated with the triazole fungicide Triticonazole. Pestic. Sci. 41: 291-295.
• Blondon F., Marie D., Brown S., Kondorosi A. (1994). Genome size and base compostion in Medicago sativa and M. truncatula species. Genome 37: 264-270.
• Bohanec B., Jakše M., Ihan A., Javornik В. (1995). Studies of gynogenesis in onion (Allium cepa L.): induction procedures and genetic analysis of regenerants. Plant Sci. 104: 215-224.
• Brown S.C., Bergounioux C., Tallet S., Marie D. (1991). Flow cytometry of nuclei for ploidy and cell cycle analysis. In: A Laboratory Guide for Cellular and Molecular Plant Biology (Ncgrutiu I., Gharti-Chhetri G. eds.). Bikhaüser, Basel: 326-345.
• Cerbah M., Coulaud J., Godelle B., Siljak-Yakovlev S. (1995). Genome size, fluorochrome banding, and karyotype evolution in some Hypochoeris species. Genome 38: 689-695.
• Cros J., Gavalda M.C., Chabrillange N., Récalt C., Duperray C., Hamon S. (1994). Variations in the total nuclear DNA content in African Coffea species (Rubiaceae). Cafe Cacao The 28: 3-10.
• Doležel J. (1991). Flow cytometric analysis of the nuclear DNA content in higher plants. Phytochcm. Analysis 2: 143-154.
• Doležel J., Doleželová M., Novák F.J. (1994). Flow cytometric estimation of the nuclear DNA amount in diploid bananas (Musa acuminata and M. balbisiana). Biol. Plant. 36: 351-357.
• Doležel J., Göhde W. (1995). Sex determination in dioecious plants Melandrium album and M. rubrum using high-resolution flow cytometry. Cytometry 19: 103-106.
• Doležel J., Lucretti S. (1995). High resolution flow karyotyping and chromosome sorting in Vicia faba lines with standard and reconstructed karyotypes. Theor. Appl. Genet. 90: 797-802.
• Doležel J., Lucretti S., Macas J. (1995). Flow cytometric analysis and sorting of plant chromosomes. In: Kew Chromosome Conference IV (Brandham P., Bennett M.D. eds.). Royal Botanic Gardens, Kew: 185-200.
• Doležel J., Lucretti S., Schubert I. (1994). Plant chromosome analysis and sorting by flow cytometry. Crit. Rev. Plant Sci. 13: 275-309.
• Doležel J., Sgorbati S., Lucretti S. (1992). Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiol. Plant. 85: 625-631.
• Duren van M., Morpurgo R., Doležel J., Afza R. (1996). Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques. Euphytica 88: 25-34.
• Dyke van M.W., Dervan P.B. (1983). Chromomycin, mithramycin, and olivomycin binding sites on heterogeneous deoxyribonucleic acid. Footprinting with (methidium-EDTA) iron (II). Biochemistry 22: 2373-2377.
• Fox M.H., Galbraith D.W. (1990). Application of flow cytometry and sorting to higher plant systems. In: Flow Cytometry and Sorting. 2nd edn., Wiley-Liss, Inc., New York: 633-650.
• Fuchs J., Houben A., Brandes A., Schubert I. (1996). Chromosome painting in plants - a feasible technique? Chromosoma 104: 315-320.
• Galbraith D.W. (1984). Flow cytometric analysis of the cell cycle. In: Cell Culture and Somatic Cell Genetics of Plants (Vasil I.K. ed.). Academic Press, New York, Vol. 1:765-777.
• Galbraith D.W. (1989). Flow cytometric analysis and sorting of somatic hybrid and transformed protoplasts. In: Plant Protoplasts and Genetic Engineering II. (Bajaj Y.P.S. ed.). Springer-Verlag, Berlin: 304-327.
• Galbraith D.W., Lambert G.M., Grebenok R.J., Sheen J. (1995). Flow cytometric analysis of transgene expression in higher plants: Green-fluorescent protein. In: Methods in Cell Biology. Academic Press, New York, Vol. 50: 3-14.
• Galbraith D.W., Harkins K.R., Maddox J.M., Ayres N.M., Sharma D.P., Firoozabady E. (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049-1051.
• Godelle B„ Cartier D., Marie D., Brown S., Siljak-Yakovlev S. (1993). Heterochromatin study demonstrating the non-linearity of fluorometry useful for calculating genomic base composition. Cytometry 14: 618-626.
• Graham M.J., Nickell C.D., Rayburn A.L. (1994): Relationsip between genome size and maturity group in soybean. Theor. Appl. Genet. 88: 429-432.
• Greilhuber J., Obermayer R. (1997). Genome size and maturity group in Glycine max. Heredity (in press).
• Heller F.O. (1973). DNS-Bestimmung an Keimwurzeln von Vida faba L. mit Hilfe der Impulscytophotometrie. Ber. Deutsch. Bot. Ges. 86: 437-441.
• Hiddeman W., Schumann J., Andreef M., Barlogie B., Herman C.J., Leif R.C., Mayall B.H., Murphy R.F., Sandberg A.A. (1984). Convention on nomenclature for DNA cytometry. Cytometry 5: 445-446.
• Huff D.R., Bara J.M. (1993). Determining genetic origins of aberrant progeny from facultative apomictic Kentucky bluegrass using a combination of flow cytometry and silver-stained RAPD markers. Theor. Appl. Genet. 87: 201-208.
• Hülgenhof E., Weidhase R.A., Schlegel R., Tewes A. (1988). Flow cytometric determination of DNA content in isolated nuclei of cereals. Genome 30: 565-569.
• Jacq B., Tétu T., Sangwan R.S., de Laat A.D., Sangwan-Norreel B.S. (1992). Plant regeneration from sugarbeet (Beta vulgaris L.) hypocotyls cultured in vitro and flow cytometric nuclear DNA analysis of regenerants. Plant Cell Reports 11: 329-333.
• Keeler K.H., Kwankin N., Barnes P.W., Galbraith D.W. (1987). Polyploid polymorphism in Andropogon gerardi. Genome 29: 374-379.
• Keller E.R.J., Schubert I., Fuchs J., Meister A. (1996). Interspecific crosses of onion with distant Allium species and characterization of the presumed hybrids by means of flow cytometry, karyotype analysis and genomic in situ hybridization. Theor. Appl. Genet. 92: 417-424.
• Kubaláková M., Doležel J., Lebeda A. (1996). Ploidy instabiliy of cmbryogenic cucumber (Cucumis sativus L.) callus culture. Biol. Plant. 38: 475-480.
• Laat de A.M.M., Blaas J. (1984). Flow-cytometric characterization and sorting of plant chromosomes. Theor. Appl. Genet. 67: 463-467.
• Laat de A.M.M., Göhde W., Vogelzang M.J.D.C. (1987). Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 99: 303-307.
• Le Pecq J.B., Paoletti G. (1967). A fluorescent complex between cthidium bromide and nucleic acids. Physical-chemical characterization. J. Mol. Biol. 27: 87-106.
• Lucretti S., Doležel J. (1995). Cell cycle synchronization, chromosome isolation, and flow-sorting in plants. In: Methods In Cell Biology (Galbraith D.W., Bourque D.P., Bohnert H.J. eds.). Academic Press, Inc., New York, Vol. 50: 59-81.
• Lysák M.A., Číhalíková J., Doležel J. (1997). Morphometric and karyological analysis of a population of Sesleria sadleriana Janka in the Biele Karpaty Mountains (Slovakia). Folia Gcobot. Phytotax. 32: 47-55.
• Macas J., Doležel J., Gualberti G., Pich U., Schubert I., Lucretti S. (1995). Primer-induced labelling of pea and broad bean chromosomes in situ and in suspension. BioTcchniqucs 19: 402-408.
• Macas J., Doležel J., Lucretti S., Pich U., Meister A., Fuchs J., Schubert I. (1993). Localization of seed storage protein genes on flow-sorted field bean chromosomes. Chrom. Res. 1: 107-115.
• Marie D., Brown S.C. (1993). A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biol. Cell 78: 41-51.
• Martínez C.P., Arumuganathan K., Kikuchi H., Earle E.D. (1994). Nuclear DNA content of ten rice species as determined by flow cytometry. Jpn. J. Genet. 69: 513-523.
• Mc Murphy L.M., Rayburn A.L. (1993). Nuclear alternations of maize plants grown in soil contaminated with coal fly ash. Arch. Environ. Contam. Toxicol. 25: 520-524.
• Misset M.T., Gourret J.P. (1995). Flow cytometric analysis of the different ploidy levels observed in the genus Ulex L. (Faboideae-Genisteae) in Brittany (France). Bot. Acta 109: 72-79.
• Nakamura D., Tiersch T.R., Douglass M., Chandler R.W. (1990). Rapid identification of sex in birds by flow cytometry. Cytogenet. Cell Genet. 53: 201-205.
• O’brien I.E.W., Smith D.R., Gardner R.C., Murray B.G. (1996). Flow cytometric determination of genome size in Pinus. Plant Sci. 115: 91-99.
• Оtto F. (1990). DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. In: Methods in Cell Biology (Crissman H.A., Darzynkiewicz Z., eds.). Academic Press, New York, Vol. 33: 105-110.
• Pich U., Meister A., Macas J., Doležel J., Lucretti S., Schubert I. (1995). Primed in situ labelling facilitates flow sorting of similar sized chromosomes. Plant Journal 7: 1039-1044.
• Pfosser M. (1989). Improved method for critical comparison of cell cycle data of asynchronously diving and synchronized cell cultures of Nicotiana tabacum. J. Plant Physiol. 134:741-745.
• Pfosser M., Amon A., Lelley T., Heberle-Bors E. (1995). Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines. Cytometry 21: 387-393.
• Portugal J., Waring M.J. (1988). Assignment of DNA binding sites for4’,6-diamidino-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochem. Biophys. Acta 949: 158-168.
• Puite K.J., Ten Broeke W.R.R. (1983). DNA staining of fixed and non-fixed plant protoplasts for flow cytometry using Hoechst 33342. Plant Sci. Lett. 32: 79-88.
• Rasch E.M. (1985). DNA "standards" and the range of accurate DNA estimates by Feulgen absorption microspectrophotometry. In: Advances in Microscopy (Cowden R.R., Harrison F.W. eds.). Alan R. Liss, Inc., New York: 137-166.
• Renno J.F., Schmelzer G.H.,de Jing J.H. (1995). Variation and geographical distribution of ploidy levels in Pennisetum section Brevivalvula (Poaceac) in Burkina Faso, Benin and southern Niger. PI. Syst. Evol. 198: 89-100.
• Sabharwal P.S., Doležel J. (1993). Interspecific hybridization in Brassica: Application of flow cytometry for analysis of ploidy and genome composition in hybrid plants. Biol. Plant. 35: 169-177.
• Schweizer L., Yerk-Davis G.L., Phillips R.L., Srienc F., Jones R.J. (1995). Dynamics of maize endosperm development and DNA endoreplication. Proc. Natl. Acad. Sci. USA 92: 7070-7074.
• Selvan E., Thomas P. (1995). Application of flow cytometric DNA measurements in the detection of irradiated onions. J. Sci. Food Agric. 67: 293-297.
• Sgorbati S., Levi M., Sparvoli E., Trezzi F., Lucchini G. (1986). Cytometry and flow cytometry of 4’,6-diamidino-2-phenylindole (DAPI)-stained suspensions of nuclei released from fresh and fixed tissues of plants. Physiol. Plant. 68: 471-476.
• Śliwińska E., Steen P. (1995). Flow cytometric estimation of ploidy in anisoploid sugar-beet populations. J. Appl. Genet. 36: 111-118.
• Tiersch T.R., Chandler R.W., Wachtel S.S., Elias S. (1989). Reference standards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry 10: 706-710.
• Tuyl van J.M., de Vries J.N., Bino R.J., Kwakkenbos T.A.M. (1989). Identification of 2n-pollen producing interspecific hybrids of Lolium using flow cytometry. Cytologia 54: 737-745.
• Ulrich I., Ulrich W. (1991). High-resolution flow cytometry of nuclear DNA in higher plants. Protoplasma 165: 212-215.
• Ulrich I., Fritz B., Ulrich W. (1988). Application of DNA fluorochromes for flow cytometric DNA analysis of plant protoplasts. Plant Sci. 55: 151-158.
• Vindeløv L.L., Christensen I.J., Nissen N.I. (1983). Standardization of high-resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standards. Cytometry 3: 328-331.
• Wang M.L., LeitchA.R., Schwarzacher T., Heslop-Harrison J.S., Moore G. (1992). Construction of a chromosome-enriched HpaII library from flow-sorted wheat chromosomes. Nucleic Acids Research 20: 1897-1901.