Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene

• Autorzy: Pietrowska M. , Rusin M. , Widlak P. , Razin S.V. , Rzeszowska-Wolny J.
• Języki publikacji: EN

Abstrakty

EN

We have analyzed the DNA fragment localized about 11 to 17.5 kb upstream of the chicken α-globin gene domain (the fragment was designed as α-0). The nucleotide sequence of its 3.3 kb-long 5' part was established and interactions with nuclear matrix proteins were studied. The DNA region localized about 16 kb upstream of the embryonic π-globin gene showed high affinity to nuclear matrices in vitro. Two palindromes and a cluster of inverted repeats were co-localized in the same region. The whole 6.6 kb α-0 fragment decreased the activity of CAT reporter gene when transfected into chicken erythroblastoid cells.

Słowa kluczowe

EN

DNA sequence; erythroblastoid cell; pi-globin gene; regulatory sequence; nuclear matrix; DNA fragment; chicken; embryo

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 3
• Opis fizyczny: p.777-784,fig.

Twórcy

autor

Pietrowska M.

• Center of Oncology, Gliwice, Poland

autor

Rusin M.

autor

Widlak P.

autor

Razin S.V.

autor

Rzeszowska-Wolny J.

Bibliografia

• Boulikas, T. (1993) Nature of DNA sequences at the attachment regions of genes to the nuclear matrix. J. Cell. Biochem. 52, 14-22.
• Boulikas, T. & Kong, C.F. (1993) Multitude of in­verted repeats characterizes a class of anchor­age sites of chromatin loops to the nuclear ma­trix. J. Cell Biochem. 53, 1-12.
• Broders, F. & Scherrer, K. (1987) Transcription of the a-globin gene domain in normal and AEV-transformed chicken erythroblasts: Mapping of giant globin-specific RNA includ­ing embryonic and adult gene. Mol Gen. Genet. 209, 210-220.
• Broders, F., Zahraoni, A. & Scherrer, K. (1990) The chicken a-globin gene domain is tran­scribed into a 17-kilobase polycistronic RNA. Proc. NatL Acad. ScL U.S.A. 87, 503-507.
• Cockerill, P.N. & Garrard, W.T. (1986) Chromo­somal loop anchorage of the kappa immuno­globulin gene occurs next to the enhancer in a region containing topoisomerase II sites. Cell 44, 273-282.
• De Moura Gallo, C.V., Vassetzky, Y.S., Huesca, M. & Scherrer, K. (1992) A transcription-depend- ent Dnase I-hypersensitive site in a far up­stream segment of the chicken a-globin gene domain coincides with a matrix attachment re­gion. Biochem. Biophys. Res. Commun. 184, 1181-1189.
• Farache, G., Razin, S.V., Rzeszowska-Wolny, «J., Moreau, J., Recillas Targa, F. & Scherrer, K. (1990) Mapping of the structural and tran­scription-related matrix attachment sites in the a-globin gene domain- of avian erythro­blasts and erythrocytes. Mol Cell Biol. 10, 5349-5458.
• Grosveld. F.. Blom van Assendelft, M., Greaves. D.R. & Kollias, G. (1987) Position-independent high-level expression of the human ^-globin gene in transgenic mice. Cell 51, 975-985.
• Characterization of DNA sequences Ioudinkova, E., Lagarkova, M.A. & Razin, S.V. (1997) Molecular cloning and characteristics of the 5'-terminal end of alpha-globin genes of chickens including potential regulator element of domain level. DokL Akad. Nauk. 356, 407-408.
• Liebehaber, S.A. & Russel, J.E. (1998) Expression and developmental control of the human alpha globin gene cluster. Ann. N.Y. Acad. Sci. 860, 54-63.
• Razin, S.V., Vassetzky, Y.S., Jr., Kvartskhava, A J., Grinenko, N.F. & Georgiev, G.P. (1991) Transcriptional enhancer in the vicinity of a replication origin within the 5' region of the chicken a-globin domain. J. Mol Biol. 217, 595-598.
• Razin, S.V., De Moura Gallo, C.V. & Scherrer, K. (1994) Characterization of the chromatin structure in the upstream area of the chicken a-globin gene domain. Mol Gen. Genet. 242, 649-652.
• Razin, S.V., Shen, K., Ioudinkova, E. & Scherrer, K. (1999) Functional analysis of DNA se­quences located within a cluster of Dnase I hy­persensitive sites colocalizing with a MAR ele­ment at the upstream border of the chicken a-globin gene domain. J. Cell. Biochem.74, 38-49.
• Recillas Targa, F. & De Moura Gallo, C.V. (1993) Analysis of the distribution of protein binding DNA motives in the vicinity of the 3'-side chicken a-globin enhancer. Biochem. Biophys. Res. Commun. 190, 1163-1172.
• Recillas Targa, F., Razin, S.V., De Moura Gallo, C.V. & Scherrer, K. (1994) Excision close to matrix attachment regions of the entrie chicken a-globin gene domain by nuclease Si and characterization of framing structures. Proc. NatL Acad. ScL U.S.A. 91, 4422-4426.
• Sambrook, J., Fritach, E.F. & Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
• Sjakste, N., Jarovaia, O., Linares-Cruz, G., De Moura Gallo, C.V. & Scherrer, K. (1998) Tran­scription of the chicken alpha-globin domain; in The Wilhelm Bernhard Workshop on the Cell Nucleus, Quebec, Canada, pp. 58-59.
• Wijgerde, M., Grosveld, F. & Fraser, P. (1995) Transcription complex stability and chroma­tin dynamics in vivo. Nature 377, 209-213.