Applications of impedance spectroscopy in biochemistry and biophysics

• Autorzy: Janshoff A. , Wegener J. , Steinem C. , Sieber M. , Galla H.J.
• Języki publikacji: EN

Abstrakty

EN

The present study is intended to demonstrate the application of impedance spectroscopy to two very different fields of biophysical research. The core component of our measuring setup is a self-constructed continuous wave impedance spectrometer together with special measuring chambers which are individually designed for the systems under investigation. We directed our attention towards: i) the investigation of solid supported lipid bilayers in general, especially systems which are suitable for protein reconstitution such as dimethyldioctadecylammonium bromide (DODAB) immobilized onto a gold electrode, precovered with a negatively charged monolayer of 3-mercaptopropionic acid. Impedance spectroscopy allows to study the stability, the thickness and the electrode coverage of those artificial membranes as well as the observation of ion transport mediated by ionophores like gramicidin D incorporated into a DODAB-bilayer. ii) The characterization of the passive electrical properties of epithelial and endothelial cell monolayers in general and especially the determination of their transepithelial (transendothelial) electrical resistances as a measure for epithelial barrier function. From impedance spectra, as reported here, we are able to follow the formation and modulation of cell layer permeability to small ions.

Słowa kluczowe

EN

cell monolayer; gramicidin; biochemistry; dimethyldioctadecylammonium bromide; impedance spectroscopy; transepithelial resistance; biophysics; lipid layer

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 2
• Opis fizyczny: p.339-348,fig.

Twórcy

autor

Janshoff A.

• Westfalische Wilhelms-Universitat Munster, Wilhelm-Klemm-Str.2, 48149 Munster, Germany

autor

Wegener J.

autor

Steinem C.

autor

Sieber M.

autor

Galla H.J.

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