Genetic polymorphism of Polish strains of Gremmeniella abietina and Brunchorstia pinea var. cembrae

• Autorzy: Kraj W
• Języki publikacji: EN

Abstrakty

EN

Thirty-three type A strains of G. abietina from diseased shoots or needles of P. sylvestris, P. nigra and P. armandii and three strains of Brunchorstia pinea var. cembrae from P. mugo were isolated from four regions of Poland differing with respect to climatic conditions. Genetic polymorphism of the mitochondrial small subunit rRNA (mtSSU rRNA), ribosomal RNA fragment including ITS1, 5.8S and ITS2 and glyceraldehyde phosphate dehydrogenase (GPD) gene was examined by the PCR-RFLP method. Genetic distance was ascertained with respect to B. pinea var. cembrae strains from G. abietina isolated from the examinedpine species (average Nei coefficient 0.137). The smallest genetic distance occurred between the strain groups of G. abietina isolated from P. nigra and P. armandii (0.059) and P. nigra and P. sylvestris (0.061), whereas the highest occurred between the groups of strains deriving from P. armandii and P. sylvestris (0.096). The impact of geographic distance on genetic distance between groups of strains from individual regions has been shown. G. abietina strains originating from mountainous areas were more distanced genetically (on average 0.031) from populations from other regions (Nei genetic distance 0.023). The main factors influencing genetic differences of the pathogen were specificity with respect to the species of the host plant and climate conditions, whereas geographic distance had lesser significance.

Słowa kluczowe

EN

coniferous tree; plant pathology; pine; spruce; fir; juniper; pathogen; plant disease; shoot blight; canker; branch; stem; Gremmeniella abietina; Brunchorstia pinea var.cembrae; needle; genetic polymorphism; Polish strain; host preference; polymerase chain reaction; RFLP analysis

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2009
• Tom: 61
• Opis fizyczny: p.13-21,fig.,ref.

Twórcy

autor

Kraj W

• Agricultural University, Al.29 Listopada 46, 31-425 Krakow, Poland

Bibliografia

• Bernier L., Hamelin R.C., Ouellette G.B. 1994. Comparison of ribosomal DNA length andrestriction site polymorphisms in Gremmeniella and Ascocalyx isolates. AppliedandEnvironmental Microbiology 60: 1279–1286.
• Bruns T.D., White T.J., Taylor J.W. 1991. Fungal molecular systematics. Annual Review of Ecology and Systematics 22: 525–564.
• Carlson J.E., Tulsieram L.K., Glaubitz J.C., Luk V.W.K., Kauffeldt C., Rutledge R. 1991. Segregation of random amplified DNA markers in F1 progeny of conifers. Theoretical andAppliedGenetics 83: 194–200.
• Donaubauer E. 1972. Distribution andhosts of Scleroderris lagerbergii in Europe andNorth America. European Journal of Forest Pathology 2: 6–11.
• Donaubauer E. 1974. Dieback of Pines in Austria causedby Scleroderris lagerbergii and Cenangium ferruginosum. 100 Jahre Forstliche Bundesversuchsanstalt. Mitteilungen der Forstlichen Bundesversuchsanstalt, Wien, pp. 67–98.
• Dorworth C.E., Krywienczyk J. 1975. Comparisons among isolates of Gremmeniella abietina by means of growth rate, conidia measurement, and immunogenic reaction. Canadian Journal of Botany 53: 2506–2525.
• Dusabenyagasani M., Laflamme G., Hamelin R.C. 2002. Nucleotide polymorphisms in three genes support host andgeographic speciation in tree pathogens belonging to Gremmeniella spp. Canadian Journal of Botany 80: 1151–1159.
• Excoffier L., Smouse P.E., Quattro J.M. 1992. Analysis of molecular variance inferredfrom metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 479–491.
• Glass N.L., Donaldson G.C. 1995. Development of primer sets designed for use with the PCR to amplify conservedgenes from filamentous ascomycetes. AppliedandEnvironmental Microbiology 61: 1323–1330.
• Guarro J., Gene J., Stchigel A.M. 1999. Developments in fungal taxonomy. Clinical Microbiology Reviews 12: 454–500.
• Hamelin R.C., Lecours N., Laflamme G. 1998. Molecular evidence of distinct introductions of the European race of Gremmeniella abietina into North America. Phytopathology 88: 582–588.
• Hamelin R.C., Lecours N., Hansson P., Hellgren M., Laflamme G. 1996. Genetic differentiation within European race of Gremmeniella abietina. Mycological Research 100: 49–56.
• Hamelin R.C., Ouellette G.B., Bernier L. 1993. Identification of Gremmeniella abietina races with random amplified polymorphic DNA markers. AppliedandEnvironmental Microbiology 59: 1752–1755.
• Hansson P., Wang X.R., Szmidt A.E., Karlman M. 1996. RAPD variation in Gremmeniella abietina attacking Pinus sylvestris and Pinus contorta in northern Sweden. European Journal of Forest Pathology 26: 45–55.
• Hantula J., Müller M. 1997. Variation within Gremmeniella abietina in Finlandandother countries as determinedby Random AmplifiedMicrosatellites (RAMS). Mycological Research 101: 169–175.
• Hedrick P.W. 2000. Genetics of populations. Jones and Bartlett Publishers, Boston.
• Hellgren M. 1995. Comparison of Gremmeniella abietina isolates from Pinus sylvestris and Pinus contorta in terms of conidial morphology and host colonization. European Journal of Forest Pathology 25: 159–168.
• Hellgren M., Högberg N. 1995. Ecotypic variation of Gremmeniella abietina in northern Europe – disease patterns reflectedby DNA variation. Canadian Journal of Botany 73: 1531–1539.
• Innis M.A., GelfandD.H., Sninsky J.J., White T.J. 1990. PCR protocols: A guide to methods and applications. Academic Press, San Diego.
• Kaitera J., Müller M.M., Hantula J. 1998. Occurrence of Gremmeniella abietina var. abietina large- and small-tree types in separate Scots pine stands in northern Finlandandin the Kola Peninsula, Russia. Mycological Research 102: 199–205.
• Karlman M., Hansson P., Witzell J. 1994. Scleroderris canker on lodgepole pine introduced in northern Sweden. Canadian Journal of Forest Research 24: 1948–1959.
• Kowalski T. 1987. Porażenie sosny czarnej przez Gremmeniella abietina /=Scleroderris lagerbergii/ w lasach Górnośląskiego Okręgu Przemysłowego. Sylwan 126: 31–39.
• Kowalski T. 1997. Zamieranie pędów sosny. Biblioteczka Leśniczego Wyd. Świat. 80: 1–18.
• Kowalski T. 2000. Czy Gremmeniella abietina zagrała świerkom? Głos Lasu 2: 12–13.
• Kowalski T., Domański S. 1983. Występowanie i przyczyny odwierzchołkowego zamierania pędów Pinus nigra, P. silvestris i P. strobus w niektórych drzewostanach południowej Polski w latach 1979–1980. Acta Agraria et Silvestria, ser. Silvestris 22: 19–34.
• Kraj W., Kowalski T. 2005. Identification of the polish strains of Chalara ovoidea using RAPD molecular markers. Acta Societatis Botanicorum Poloniae 74: 35–42.
• Kraj W., Kowalski T. 2008. Genetic variation of polish strains of Gremmeniella abietina. Forest Pathology 38: 203–217.
• Laflamme G. 1993. Scleroderris canker, North American andEuropean strains in Canada. In: Canker andShoot Blight of Conifers. IUFRO Working Party S2.06.02 (ed. P. Barklund). pp. 59–64.
• Garpenberg Sweden, 10–15 June 1991. Lecours N., Toti L., Sieber T.N., Petrini O. 1994. Pectic enzyme patterns as a taxonomic tool for the characterization of Gremmeniella spp. isolates. Canadian Journal of Botany 72: 891–896.
• Morelet M. 1980. La maladie a Brunchorstia: Position systematique et nomenclature du pathogen. European Journal of Forest Pathology 10: 268–277.
• Müller M.M., Uotila A. 1997. The diversity of Gremmeniella abietina var. abietina FAST-profiles. Mycological Research 101: 557–564.
• Nei M. 1972. Genetic distance between populations. American Naturalist 106: 283–292.
• Peakall R., Smouse P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching andresearch. Molecular Ecology Notes 6: 288–295.
• Petrini O., Petrini L.E., Laflamme G., Ouellette G.B. 1989. Taxonomic position of Gremmeniella abietina and relatedspecies: a reappraisal. Canadian Journal of Botany 67: 2805–2814.
• Petrini O., Toti L., Petrini L.E., Heiniger U. 1990. Gremmeniella abietina and G. laricina in Europe: characterization andid entification of isolates and laboratory strains by soluble protein electrophoresis. Canadian Journal of Botany 68: 2629–2635.
• Roll-Hansen F., Roll-Hansen H. 1973. Scleroderris lagerbergii in Norway. Hosts, distribution, perfect and imperfect state and mode of attack. Meddelelser fra Det norske Skogforsøksvesen 30: 441–459.
• Santamaria O., Alves-Santos F.M., Diez J.J. 2005. Genetic characterization of Gremmeniella abietina var. abietina isolates from Spain. Plant Pathology 54: 331–338.
• Schwarz F. 1895. Die Erkrankung der Kiefern durch Cenangium abietis. Verlag Fischer, Jena.
• Sierota Z. 1998. Choroby infekcyjne – ocena występowania i wpływ na gospodarkę leśną. Sylwan 1, 21–37.
• Skilling D.D. 1969. Spore dispersal by Scleroderris lagerbergii under nursery and plantation conditions. Plant Disease Reporter 53: 291–295.
• Skilling D.D. 1993. The life cycle of Gremmeniella abietina in different countries and continents – an overview. In: Canker andShoot Blight of Conifers. IUFRO Working Party S2.06.02 (ed. P. Barklund). pp. 7–18. Garpenberg Sweden, 10–15 June 1991.
• Uotila A., Kurkela T., Tuomivirta T., Hantula J., Kaitera J. 2006. Gremmeniella abietina types cannot be distinguished using ascospore morphology. Forest Pathology 36: 395–405.
• Wang X.R., Ennos R.A., Szmidt A.E., Hansson P. 1997. Genetic variability in the canker pathogen fungus, Gremmeniella abietina. 2. Fine-scale investigation of the population genetic structure. Canadian Journal of Botany 75: 1460–1469.
• Yokota S. 1975. Scleroderris canker of Todo-fir in Hokkaido, Northern Japan. III. Dormant infection of the causal fungus. European Journal of Forest Pathology 5: 7–12.
• Yokota S., Uozumi T., Matsuzaki S. 1974. Scleroderris canker of Todo-fir in Hokkaido, Northern Japan. II. Physiological and pathological characteristics of the causal fungus. European Journal of Forest Pathology 4: 155–166.