Beta-adrenergic stimulation does not influence the activity of lymphocyte Kv1.3 channels

• Autorzy: Teisseyre A. , Gawlik A. , Krasnowska M.
• Języki publikacji: EN

Abstrakty

EN

The aim of our study was to clarify the still controversial problem concerning the modulatory effect of β-adrenergic stimulation on the activity of Kv1.3 channels in human T Lymphocytes. Because the expression of β-adrenergic receptors in T Lymphocytes is significantly altered in patients with bronchial asthma we examined the cells taken both from healthy donors and asthmatic patients. We applied the whole-cell patch-clamp technique to study the modulatory effect of β-adrenergic stimulation on the whole-cell potassium conductance, gating and kinetics of Lymphocyte Kv1.3 channels. During the experiments β-adrenergic agonist Isoprenaline was applied at concentrations up to 10-4 M. It was shown that the activity of T lymphocyte Kv1.3 channels remain unchanged upon β-adrenergic stimulation both in cells taken healthy donors and asthmatic patients. Results of our investigations support the notion that β- adrenergic stimulation does not modulate the activity of Kv1.3 channels in human TL. The transient increase in T lymphocyte K+ channel activity upon β-adrenergic stimulation that has been reported in some previous studies is most probably due to an activation of recently identified, voltage-independent cAMP-responsive K+ channels.

Słowa kluczowe

EN

T lymphocyte; man; asthmatic patient; patch clamp; lymphocyte; beta-adrenergic stimulation; beta-adrenergic receptor; potassium channel; bronchial asthma; isoprenaline

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1997
• Tom: 02
• Numer: 3
• Opis fizyczny: p.301-315,fig.

Twórcy

autor

Teisseyre A.

• Wroclaw University of Medicine, ul.Chalubinskiego 10, 50-368 Wroclaw, Poland

autor

Gawlik A.

autor

Krasnowska M.

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