Cloning and characterization of Arabidopsis thaliana AtNAP57-a homologue of yeast pseudouridine synthase Cbf5p

• Autorzy: Maceluch J. , Kmieciak M. , Szweykowska-Kulinska Z.
• Języki publikacji: EN

Abstrakty

EN

Rat Nap57 and its yeast homologue Cbf5p are pseudouridine synthases involved in rRNA biogenesis, localized in the nucleolus. These proteins, together with H/ACA class of snoRNAs compose snoRNP particles, in which snoRNA guides the synthase to direct site-specific pseudouridylation of rRNA. In this paper we present an Arabidopsis thaliana protein that is highly homologous to Cbf5p (72% identity and 85% homology) and NAP57 (67% identity and 81% homology). Moreover, the plant protein has conserved structural motifs that are characteristic features of pseudouridine synthases of the TruB class. We have named the cloned and character­ized protein AtNAP57 (Arabidopsis thaliana homologue of NAP57). AtNAP57 is a 565 amino-acid protein and its calculated molecular mass is 63 kDa. The protein is en­coded by a single copy gene located on chromosome 3 of the A. thaliana genome. Inter­estingly, the AtNAP57 gene does not contain any introns. Mutations in the human DKC1 gene encoding dyskerin (human homologue of yeast Cbf5p and rat NAP57) cause dyskeratosis congenita a rare inherited bone marrow failure syndrome charac­terized by abnormal skin pigmentation, nail dystrophy and mucosal leukoplakia.

Słowa kluczowe

EN

yeast; nucleolus; rRNA; bipartite nuclear localization signal; human telomerase RNA; cloning; biogenesis; Arabidopsis thaliana; protein; pseudouridine synthase; cDNA

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 3
• Opis fizyczny: p.699-709,fig.

Twórcy

autor

Maceluch J.

• Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Kmieciak M.

autor

Szweykowska-Kulinska Z.

Bibliografia

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