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2010 | 79 | 2 |

Tytuł artykułu

Effect of meta-topolin in vitro propagation of Pelargonium x hortorum and Pelargonium x hederaefolium cultivars

Autorzy

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
July and September. The most efficient regeneration and axillary multiplication were achieved on the medium supplemented with meta-topolin. The application of BAP caused a lower regeneration potency of explants and resulted in a decrease of shoot quality with every subculture. Four of the six cultivars showed growth inhibition after three months of growth on BAP-medium. The highest multiplication rate (2.7-4.7 depending on genotype) and the high quality of shoots were noted on the medium supplemented with mT (0.5-1.0 mg l-1). It is also very important to note that mT had stimulating effect on organogenesis in P. × hederaefolium and P. × hortorum cultivars over the long term. Moreover, meta-topolin had no after-effect on the growth and inhibition of rooting. Only one cultivar ("Sofie Cascade") rooted better on control medium without auxin. In case of the other cultivars, IBA added in concentrations of 0.01-0.1 mg l-1 had a stimulating effect on root production. The higher level of auxin inhibited root formation, stimulated senescence of shoots and had a negative after-effect on acclimatization in greenhouse conditions.

Wydawca

-

Rocznik

Tom

79

Numer

2

Opis fizyczny

p.101-106,fig.,ref.

Twórcy

autor
  • Szczepan Pieniazek Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 Skierniewice, Poland

Bibliografia

  • BAIRU M.W., STIRK W.A., DOLEZAL K., VAN STADEN J. 2007. Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell Tiss. Org. Cult. 90: 15-23.
  • BAROJA-FERNÁNDEZ E., AGUIRREOLEA J., MARTÍNKO- VÁ H., HANUŚ J., STRNAD M. 2002. Aromatic cytokinins in micropropagated potato plants. Plant Physiol. Biochem. 40: 217-224.
  • CAG S., PALAVAN-ÜNSAL N. 2003. The effect of meta-topolin on protein profile in radish cotyledons. J. Cell Mol. Biol. 2: 31-34.
  • CASSELLS A.C., MINAS G. 1983. Plant and in vitro factor influencing the micropropagation of Pelargonium cultivars by bud-tip culture. Scientia Hortic. 21: 53-61.
  • CASSELLS A.C., CARNEY B.F. 1987. Adventitious regeneration in Pelargonium X domesticum Bailey. Acta Hortic. 212: 419-424.
  • CORNEANU M., CORNEANU G.C. 1991. The genotype, explant type and medium composition influence on in vitro multiplication in Pelargonium sp. Acta Hortic. 289: 101-102.
  • DEL POZO J.C., LOPEZ-MATAS M.A., RAMIREZ-PARRA E., GUTIERREZ C. 2005. Hormonal control of the plant cycle. Physiol. Plant. 123: 173-183.
  • DESILETS H., DESJARDINS Y., BELANGER R. 1993. Clonal propagation of Pelargonium X hortorum through tissue culture: Effects of salt dilution and growth regulator concentration. Can. J. Plant Sci. 73: 871-878.
  • DOBRÁNSZKI J., MAGYAR-TÁBORI K., JÁMBOR- BENCZÚR E., KISS E., LAZÁNYI J., BUBÁN T. 2002. Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves. Acta Agron. Hung. 50: 191-195.
  • ESCALONA M., CEJAS I., GONZÁLES-OLMEDO J., CAPOTE I., ROELS S., CAÑAL M.J., RODRÍGEZ R., SANDOVAL J., DEBERGH P. 2003. The effect of meta--topolin on plantain propagation using a temporary immersion bioreactor. InfoMusa 12:28-30.
  • GASPAR TH., KEVERS C., FAIVRE-RAMPANT., CREVECO- EUR M., PENEL CL., GREPPIN H., DOMMES J. 2003. Changing concepts in plant hormone action. In Vitro Cell. Biol.-Plant 39: 85-106.
  • HAMDORF G. 1976. Propagation of Pelargonium varieties by stem-tip culture. Acta Hortic. 59 (Abstr.): www.actahort.org.
  • HEYL A., SCHMÜLLING T. 2003. Cytokinin signal perception and transduction. Current Opin. Plant Biol. 6: 480-488.
  • HOLUB J., HANUŚ J., HANKE D.E., STRNAD M. 1998. Biological activity of cytokinins derived from orto- and meta-hydro-xybenzyladenine. Plant Growth Regul. 28: 109-115.
  • HORN W. 1988. Micropropagation of Pelargonium X domesticum (P.grandiflorum - hybrids). Acta Hortic. 226: 54-58.
  • KAMINEK M., VANÉK T., MOTYKA V. 1987. Cytokinin avti- vities of N6-benzyladenosine derivatives hydroxylated on the side-chain phenyl ring. J. Plant Growth Regul. 6: 113-120.
  • KUBALÁKOVÁ M., STRNAD M. 1992. The effect of aromatic cytokinins on micropropagation and regeneration of sugarbeet in vitro. Biol. Plant. 34: 578-579.
  • MITHILA J., MURCH S.J., KRISHNARAJ S., SAXENA P.K. 2001. Recent advances in Pelargonium in vitro regeneration system. Plant Cell Tiss. Org. Cult. 67: 1-9.
  • MOK M.C., MARTIN R.C., DOBREV P.I., VANKOVÁ R., SHING HO P., YONEKURA-SAKAKIBARA K., SAKAKI- BARA H., MOK D.W.S. 2005. Topolins and hydroxylated thi- diazuron derivatives are substrates of cytokinin o-glucosyl- transferase with position specificity related to receptor recognition. Plant Physiol. 137: 1057-1066.
  • MURASHIGE T., SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
  • PALAVAN-ÜNSAL N., ÇAG S., ÇETIN E. 2004. The role of meta-topolin in senescence of wheat leaf segments. J. Cell Mol. Biol. 3: 23-31.
  • PALUDAN N. 1991. Micropropagation, plantlet survival and variation of virus-infected and virus-free Pelargonium x horto- rum and P. peltatum. In: Bridge, A report of Cost 87, (ed.) M. Appelgren, C.S. Hunter, N. Paludan, G. Reuther, R.T. Theiler- Hedtrich: 60-69.
  • PODWYSZYŃSKA M., WOJTANIA A., GABRYSZEWSKA E. 2000. M-topolina - mało znana cytokinina w mikrorozmnaża- niu roślin. Zesz. Nauk. Inst. Sadow. i Kwiac. 7: 173-180. (in Polish)
  • REUTHER G. 1983. Propagation of disease-free pelargonium cultivars by tissue culture. Acta Hortic. 131: 311-319.
  • REUTHER G. 1988. Variability of Pelargonium peltatum cultivar in long-term in vitro culture of disease-free nucleus stock. Acta Hortic. 226: 647-654.
  • STRNAD M., PETERS W., BECK E., KAMINEK M. 1992. Immunodetection and identification of N6-(o-hydroxybenzulami- no) purine as naturally occurring cytokinin in Populus x canadensis cv. Robusta leaves. Plant Physiol. 99: 74-80.
  • STRNAD M. 1997. The aromatic cytokinins. Physiol. Plant. 101: 674-688.
  • THEILER R. 1977. In vitro culture of shoot tips of Pelargonium species. Acta Hortic. 78: 403- 412.
  • WERBROUCK S.P.O., STRNAD M., VAN ONCKELEN H.A., DEBERGH P.C. 1996. Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol. Plant. 98: 291-297.
  • WOJTANIA A., GABRYSZEWSKA E. 2001. Effect of cytoki- nins and amino acids on multiplication of Pelargonium cultivars. Acta Soc. Bot. Pol. 70: 203-207.
  • WOJTANIA A., GABRYSZEWSKA E. 2004. Wykorzystanie metatopoliny w procesie wzrostu i rozwoju pędów Pelargonium X hortorum in vitro. Biotechnologia 2: 162-167.

Typ dokumentu

Bibliografia

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