PL
Laseczka wąglika posiada szereg genów bezpośrednio lub pośrednio związanych ze zjadliwością, zlokalizowanych zarówno w plazmidach jak też w chromosomie. Na podstawie wyników PCR ze starterami swoistymi dla ośmiu genów (capA, capB, capC, pagA, lef, cya, gerXA i sap) kodujących potencjalne czynniki wirulencji określono wirulotypy szczepów Bacillus anthracis izolowanych na terenie kraju.
EN
Bacillus anthracis - the causative agent of anthrax - possesses several virulence genes located in the chromosome as well as in two B. anthracis virulence plasmids: pXOl and pX02. In the presented study, we determined occurrence of six virulence markers located in the virulence plasmids (capA, capB, capC, pagA, lef and cyd) for capsule and toxin production together with virulence-associated gene gerXA and chromosomal gene sap, which are responsible for germination and S-layer biosynthesis respectively. Fourteen strains of B. anthracis isolated in Poland and belonging to five different MLVA genotypes were analyzed by PCR for presence of the aforementioned genes. Two virulotypes were found in tested strains. The only variation was absence of capA, capB and capC due to a lack of pX02.