The effect of toxic cyanobacteria [blue-green algae] on water plants and animal cells

• Autorzy: Romanowska-Duda Z , Mankiewicz J. , Tarczynska M. , Walter Z. , Zalewski M.
• Języki publikacji: EN

Abstrakty

EN

The eutrophication of the Sulejów reservoir dam in Poland is connected with the problem of toxicity of cyanobacterial blooming (blue-green algal blooming). The main species responsible for hepatotoxic ”algal bloom” formation is Microcystis aeruginosa. The aim of this study is to evaluate the influence of the toxic cyanobacterial extract containing microcystins on the growth and morphology of a water plant (Spirodela oligorrhiza) and animal cells (rat hepatocytes). A higher concentration of cyanobacterial extract (MC-LR = 343 µg/dm3) reduced the number of fronds by about 50% in comparison with the control. The extract affected the reduction mass of fronds and the concentration of chlorophyll. The activity of the constitutive acid phosphatase decreased. The first morphological changes in rat hepatocytes typical of apoptosis were observed after 30 minutes of incubation with the cyanobacterial extract. The hepatocytes underwent cell membrane blebbing (MC-LR = 100 µg/dm3). The next 30 minutes of incubation caused an increase in the percentage of deforming cells of more than 50% (MC-LR = 100 µg/dm3). High chromatin condensation and apoptotic bodes were observed in 90% of cells after 120 minutes (MC-LR . 500 µg/dm3). The results of studies confirm the high toxic and cytotoxic effect of blue-green algal blooming from Sulejów reservoir on both plants and animals.

Słowa kluczowe

EN

Cyanoprokaryota; alga; Poland; plant growth; Spirodela oligorrhiza; animal cell; rat; Microcystis aeruginosa; morphological change; blue-green alga; Aphanizomenon flos-aquae; toxicity; growth regulation; bloom; hepatocyte; aquatic plant; Sulejow Reservoir; eutrophication

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2002
• Tom: 11
• Numer: 5
• Opis fizyczny: p.561-566,fig.

Twórcy

autor

Romanowska-Duda Z

• University of Lodz, Banacha 12-16, 90-237 Lodz, Poland

autor

Mankiewicz J.

autor

Tarczynska M.

autor

Walter Z.

autor

Zalewski M.

Bibliografia

• 1. BELL S.G., CODD GA. Detection, Analysis and Risk Assessment of Cyanobacterial Toxins. In: Agricultural Chemicals and the Environment, ed.; The Royal Society of Chemistry, Cambridge, UK, pp 109-122, 1996.
• 2. BOLLARD E.A. Comparative study of the ability of organic nitrogenous compounds to serve as sole source of nitrogen for the growth of plants. Plant & Soil 25, 153, 1966.
• 3. CARMICHAEL W.W. Cyanobacteria secondary meta- bolites-the cyanotoxins. J. Appl. Bacteriol. 72, 460, 1992.
• 4. CARMICHAEL W.W. Toxic Microcystis and the Environ ment. In: Toxic microcystins, ed.; CRC Press, pp 1-10, 1996.
• 5. CASANOVA M.T., BURCH M.D, BROCK M.A., BOND P.M. Does toxic Microcystis aeruginosa affect aquatic plant establishment. Environ. Toxicol. 14, 97, 1999.
• 6. CHORUS I, FALCONER I.R, BARTRAM J. Health caused by freshwater cyanobacteria in recreational waters. J. Toxicol. Environ. Health 3, 323, 2000.
• 7. DAWSON R.M. The toxicology of microcystin. Toxicon 36, 956,1998.
• 8. DUY T.N., LAM P.K.S., SHAW G.R., CONNELL D.W. Toxicology and Risk Assessment of Freshwater Cyanobacterial (Blue-Green Algal) Toxins in Water. Rev. Environ. Contam. Toxicol. 163, 113, 2000.
• 9. ERIKSSON J.E. Toxic Peptides from Cyanobacteria - Characterization and Cellular Mode of Action, ed.; Department of Biology Abo Akademi University, Finland, 1990.
• 10. ERIKSSON J.E., BRAUTIGAN D.L., VALLEE R., OL­MSTED J., FUJOKI H., GOLDMAN R.D. Cytoskeletal in tegrity in interphase cells require protein phosphatase activ ity. Proc. Natl. Acad. Sci. USA, 89, 11093, 1992.
• 11. FALCONER I.R. An Overview of Problems Caused by Toxic Blue-Green Algae (Cyanobacteria) in Drinking and Recreational Water. Environ. Toxicol. 14, 5, 1999.
• 12. FAWELL J.K., MITCHELL R.E, EVERETT D.J, HILL R.E. The toxicity of cyanobacterial toxins in the mouse: I Microcystin - LR. Human Experimental Toxicology 18, 162, 1999.
• 13. FUJIKI H, SUGANUMA M. Tumor promotion by inhibitors of protein phosphatase 1 and 2A: The okadaic acid class of compounds. Adv. Cancer Res. 61, 143, 1993.
• 14. JOCHIMSEN E.M, CARMICHAEL W.W, AN J.S, CARDO DM, COOKSON ST., HOLMES C.E.M, et al. Liver failure and death after exposureto microcystins at a hemodialysis center in Brazil. New England J. Med. 338, 873, 1998.
• 15. KAYA K. Toxicology of Microcystins. In: Toxic microcystin, ed.; CRC Press, pp 175-203, 1996.
• 16. KOS P., GORZO G., SURANYI G., BORBELY G. Simple and efficient method for isolation and measurement of cy­anobacterial hepatotoxins by plant tests (Sinapis alba L.). Anal. Biochem. 225, 49, 1995.
• 17. KURKI-HELASMO K., MERILUOTO J. Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings. Toxicon 36, 1921, 1998.
• 18. LAWTON L.A., BEATTIE K.A., HAWSER S.P., CAM PBELL D.L., CODD G.A. In: Detection Methods for Cy­anobacterial Toxins, ed.; The Royal Society of Chemistry: Cambridge, UK, pp 111-116, 1994.
• 19. LEWIS M.A. Use of fresh water plants for phytotoxicity testing: a review. Environ. Pollution 87, 319, 1995.
• 20. MANKIEWICZ J., WALTER Z., TARCZYŃSKA M., ZALEWSKI M., FLADMARK K.E. et al. Apoptotic effect of cyanobacteroal blooms collected from water reservoirs. Pol. J. Occup. Med. Environ. Health 13(4), 335, 2000.
• 21. MELLGREN G, VINTERMYR O.K., DOSKELAND SO. Okadaic acid, cAMP and selected nutrients inhibit hepa- tocyte proliferation at different stages in Gl. Modulation of the cAMP effect by phosphatase inhibitors and nutrients. J. Cell Physiol. 163, 232, 1995.
• 22. PHILLIPS M.J., ROBERTS R.J., STEWART J.A., CODD, G.A. The toxicity of the cyanobacterium Microcystis aeruginosa to rainbow trout, Salmo geirdneri Richardson. J. Fish Diseases 8, 339, 1985.
• 23. PIETSCH C, WIEGAND C, AME M.V., NICKLISCH A., WUNDERLIN D., PFLUGMACHER S. The Effects of a Cyanobacterial Crude Extract on Different Aquatic Organisms: Evidence for Cyanobacterial Toxin Modulating Factors. Environ. Toxicology, 1-8, 2001.
• 24. RESSOM R, SAN SOONG F., FITZGERALD J., TUR- CZYNOWICZ L., EL SAADI O. et al. Healta Effects of Toxic Cyanomacteria (Blue - Green Algae), ed.; Australian Govertnment Publishing Service, pp 27-69, 1994.
• 25. ROMANOWSKA-DUDA, Z., SAKOWICZ T., TARCZYNSKA M, KNYPL J. S., ZALEWSKI M. Morphological changes vs changes in DNA structures in Spirodela oligorrhiza caused by Microcystin-LR. Annual Meeting ESNA, 7 - 12. IX. 1999, Wye, England, 1999.
• 26. RUNNEGAR M.T.C., GERDES R.C., FALCONER I.R. The uptake of the cyanobacterial hepatotoxin microcystin by isolated rat hepatocytes. Toxicon, 29(1), 43, 1991.
• 27. SGONC R., GRUBER J. Apotosis detection. Experimental Gerontology 6, 525, 1998.
• 28. SINGH D.P., TYAGI M.B., KUMAR A. In: Cyanobacterial Toxins, ed.; Cyanobacterial and Algal Metabolism and Environmental Biotechnology, Narosa Publishing House, New Delhi, India, pp 61-72, 1999.
• 29. SIVONEN K., NIEMELA S.I., NIEMI R.M., LEPISTO L, LUOMA T.H. Toxic cyanobacteria (blue-green algae) in Finnish fresh and coastal waters. Hydrobiol. 190, 267, 1990.
• 30. TOIVOLA D.M., ERIKSSON J.E. Toxins Affecting Cell Signalling and Alteration of Cytoskeletal Structure. Toxicology in Vitro, 13, 521, 1999.
• 31. VAUX D.L., KORSMEYER S.J. Cell death in develop ment. Cell 96, 245, 1999.
• 32. WANG W. Literatur review on duckweed toxicity testing. Environmental Research 52, 7, 1989.
• 33. WATANABE M.F., HARADA K.I., CARMICHAEL W.W., FUJIKI H. Toxic Microcistis, ed.; CRC Press, Floryda, 1996.
• 34. WEIF J., LIBERT H.P., BRAUNE W. Influece of Microcys- tin-RR on Growth and Photosynthetic Capacity of the Duckweed Lemna minor L. J. of Applied Botany 74, 100, 2000.
• 35. WYLLIE A.H. Apoptosis: an overviev. British Medical Bull etin 53(3), 451, 1997.
• 36. YOSHIDA T., MAKITA Y., TSUTSUMI T., NAGATA S., TASHIRO F., et al. Immunohistochemical Localization of Microcystin-LR in the Liver of Mice: A Study on the Pathogenesis of microcystin-LR-Induced Hepatotoxicity. Toxicologic Pathology 26(3), 41, 1998.