Expression of 5-HT1A and 5-HT7 receptors in Caco-2 cells and their role in the regulation of serotonin transporter activity

• Autorzy: Iceta R. , Mesonero J.E. , Aramayona J.J. , Alcalde A.I.
• Języki publikacji: EN

Abstrakty

EN

Serotonin (5-HT) receptors are expressed in the gastrointestinal tract and play an important role in gastrointestinal activity regulation. 5-HT binding to receptors depends on 5-HT availability, which is, in part, modulated by the 5-HT transporter (SERT) expressed in enterocytes. This work concerns the expression of 5-HT1A and 5-HT7 receptors (5-HTR1A and 5-HTR7) in the human enterocyte-like Caco-2 cell line and their role in SERT activity modulation. The results demonstrate the mRNA and protein expression of 5-HTR1A and 5-HTR7 in these cells. In addition, both receptors are shown to modulate SERT activity; 5-HTR1A activation increased 5-HT uptake and 5-HTR7 activation inhibited it. In both cases, SERT modulation might involve a cAMP/PKA pathway. Effects on SERT disappeared after long-term activation of 5-HTR1A and 5-HTR7, indicating their desensitization. However, in both cases, the desensitization did not show itself to be mediated by a reduction of the amount of receptors in the membrane.

Słowa kluczowe

EN

mRNA; serotonin transporter; protein expression; Caco-2 cell line; 5-HT1A receptor; 5-HT7 receptor; gastrointestinal tract; serotonin

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 1
• Opis fizyczny: p.157-164,fig.,ref.

Twórcy

autor

Iceta R.

• University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain

autor

Mesonero J.E.

autor

Aramayona J.J.

autor

Alcalde A.I.