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2003 | 45 | 1 |

Tytuł artykułu

Cytoplasmic reserves in generative and vegetative cells of Hermodactylus tuberosus Mill.: Cytochemistry, ESI and EELS analyses

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
This study uses cytochemical tests, electron spectroscopic imaging and electron energy loss spectroscopy techniques to identify and localize the reserves inside the generative cell of Hermodactylus tuberosus pollen. Cytochemical probes applied to sections observed by light and transmission electron microscopy indicated that the generative cell contains large osmiophilic bodies probably made of phytic acid rich in P and Ca. The significance of the rich granulations in generative cells of Hermodactylus pollen is discussed in relation to floral biology and environmental conditions. In comparison, the vegetative cytoplasm contains (a) lipid droplets formed by unsaturated lipids and related to vacuoles, (b) lipid bodies with larger dimensions, irregular in shape and very rich in Ca, (c) bodies stained in polysaccharide tests as well as lipid probes tentatively identified as glycolipid granulations, and (d) small granules very rich in P and Ca interpreted as phytin granules.

Wydawca

-

Rocznik

Tom

45

Numer

1

Opis fizyczny

p.83-88,fig.

Twórcy

autor
  • University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy
autor

Bibliografia

  • Butowt R, Rodriguez-Garcia MI, Alché JD, and Gorska-Brylass A. 1997. Calcium in electron-dense globoids during pollen grain maturation in Chlorophytum elatum R. Br. Planta 203: 413-421.
  • Canini A, Leonardi D, Ruggeri S, Carnovali E, and Grilli Caiola M. 2001. Intracellular localization of calcium, phosphorus and nitrogen in common bean seeds (Phaseolus vulgaris L. cv Borlotto) by SEM, ESI and EELS techniques. Plant Biosystems 135: 123-132.
  • Cresti M, Ciampolini F, and Kapil RN. 1984. Generative cells of some angiosperms with particular emphasis on their microtubules. Journal of Submicroscopic Cytology 16: 317-326.
  • Dafni A, and Werker E. 1982. Pollination ecology of Sterubergia clusiana a (ker-gawler) spreng (Amaryllidaceae). New Phytologist 91: 571-577.
  • Dunnigan MG. 1968. The use of nile blue sulphate in the histochemical identification of phospholipids. Stain Technology 43: 249-256.
  • Farragiana T, and Marinozzi V. 1979. Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. Journal of Submicroscopic Cytology 12: 263-265.
  • Greenwood JS, and Bewley D. 1984. Subcellular distribution of phytin in the endosperm of developing castor bean: a possibility for its synthesis in the cytoplasm prior to deposition within protein bodies. Planta 160: 113-120.
  • Grilli Caiola M, and Brandizzi F. 1997. Pollen-pistil interactions in Hermodactylus tuberosus Mill. (Iridaceae). Plant Biosystems 131: 197-205.
  • Grilli Caiola M, Brandizzi F, and Canini A. 2000. Hermodactylus tuberosus L. (Iridaceae) pollen organization before and after anther dehiscence. Plant Biosystems 134: 353-364.
  • Jackson JF, Jones G, and Linskens HF. 1982. Phytic acid in pollen. Phytochemistry 21: 1255-1258.
  • Johansen DA. 1940.Plant microtechnique. Me Graw Hill, New York.
  • Knox RB, Kenrick J, Jobson S, and Dumas C. 1989. Reproductive function in the mimosoid legume Acacia retinodes: ultrastructural and cytochemical characteristics of stigma receptivity. Australian Journal of Botany 37: 103-124.
  • McCormick S. 1993. Male gametophyte development. Plant Cell 5: 1265-1275.
  • Pacini E. 1996. Types and meaning of pollen carbohydrate reserves. Sexual Plant Reproduction 9: 362-366.
  • Piffanelli P, Ross JHE, and Murphy DJ. 1998. Biogenesis and function of the lipidic structures of pollen grains. Sexual Plant Reproduction 14: 65-80.
  • Seligman A, Wasserkrung HL, and Hanker JS. 1966. A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide-fixed tissues with osmiophilic thiocarbohydrazide (TCH). Journal of Cell Biology 30: 424-432.
  • Soloff BL. 1973. Buffered potassium permanganate-uranyl acetate-lead citrate staining sequence for ultrathin sections. Stain Technology 48: 159-165.
  • Tanaka I. 1993. Development of male gametes in flowering plants. Journal of Plant Research 105: 55-63.
  • Thiéry J.P. 1967. Mise en evidence des polysaccharides sur coupes fines en microscopie électronique. Journal of Microscopie 6: 987-1018.
  • Venable JH, and Cóggeshall R. 1965. A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25: 407-408.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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